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Ndlela LL, Schmidt S. Evaluation of wild herbivore faeces from South Africa as a potential source of hydrolytically active microorganisms. Springerplus. 2016;5:118doi: 10.1186/s40064-016-1739-y.
Ndlela, L. L., & Schmidt, S. (2016). Evaluation of wild herbivore faeces from South Africa as a potential source of hydrolytically active microorganisms. SpringerPlus, 5118. https://doi.org/10.1186/s40064-016-1739-y
Ndlela, Luyanda L, and Schmidt, Stefan. "Evaluation of wild herbivore faeces from South Africa as a potential source of hydrolytically active microorganisms." SpringerPlus vol. 5 (2016): 118. doi: https://doi.org/10.1186/s40064-016-1739-y
Ndlela LL, Schmidt S. Evaluation of wild herbivore faeces from South Africa as a potential source of hydrolytically active microorganisms. Springerplus. 2016 Feb 09;5:118. doi: 10.1186/s40064-016-1739-y. eCollection 2016. PMID: 26900540; PMCID: PMC4746204.
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Springerplus. 2016 Feb 09;5:118. doi: 10.1186/s40064-016-1739-y. eCollection 2016.

Evaluation of wild herbivore faeces from South Africa as a potential source of hydrolytically active microorganisms.

SpringerPlus

Luyanda L Ndlela, Stefan Schmidt

Affiliations

  1. Discipline of Microbiology, School of Life Sciences, University of KwaZulu-Natal, Private Bag X01, Pietermaritzburg, 3209 South Africa.

PMID: 26900540 PMCID: PMC4746204 DOI: 10.1186/s40064-016-1739-y

Abstract

This study assessed faecal matter from three indigenous South African herbivores-zebra, giraffe and impala-as a potential source for hydrolytically active aerobic and facultatively anaerobic bacteria. Herbivore droppings were collected freshly in a local nature reserve in Pietermaritzburg, South Africa. Soil samples adjacent to faecal collection sites and faeces from a domestic herbivore, the Nguni cow, were included as controls. Hydrolase and dehydrogenase activity in faecal matter and soil samples were measured by the fluorescein diacetate and the triphenyltetrazolium chloride assay. Viable counts and counts for amylase, cellulase, esterase and protease producers were established using plate count agar and solid media containing cellulose, skim milk, starch and Tween 80. Zebra droppings produced the highest hydrolase and dehydrogenase activity. Faecal matter of the three indigenous herbivores generally produced higher hydrolytic activity than Nguni cow faeces and soil controls, thereby confirming that these materials are potential targets for hydrolytic enzyme mining.

Keywords: Faeces; Fluorescein diacetate; Giraffe; Hydrolases; Impala; Nguni; Triphenyltetrazolium chloride; Zebra

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