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Trentz OA, Arikketh D, Sentilnathan V, et al. Surface proteins and osteoblast markers: characterization of human adipose tissue-derived osteogenic cells. Eur J Trauma Emerg Surg. 2010;36(5):457-63doi: 10.1007/s00068-010-0030-0.
Trentz, O. A., Arikketh, D., Sentilnathan, V., Hemmi, S., Handschin, A. E., de Rosario, B., Mohandas, P., & Mohandas, P. V. (2010). Surface proteins and osteoblast markers: characterization of human adipose tissue-derived osteogenic cells. European journal of trauma and emergency surgery : official publication of the European Trauma Society, 36(5), 457-63. https://doi.org/10.1007/s00068-010-0030-0
Trentz, O A, et al. "Surface proteins and osteoblast markers: characterization of human adipose tissue-derived osteogenic cells." European journal of trauma and emergency surgery : official publication of the European Trauma Society vol. 36,5 (2010): 457-63. doi: https://doi.org/10.1007/s00068-010-0030-0
Trentz OA, Arikketh D, Sentilnathan V, Hemmi S, Handschin AE, de Rosario B, Mohandas P, Mohandas PV. Surface proteins and osteoblast markers: characterization of human adipose tissue-derived osteogenic cells. Eur J Trauma Emerg Surg. 2010 Oct;36(5):457-63. doi: 10.1007/s00068-010-0030-0. Epub 2010 Jul 14. PMID: 26816227.
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Eur J Trauma Emerg Surg. 2010 Oct;36(5):457-63. doi: 10.1007/s00068-010-0030-0. Epub 2010 Jul 14.

Surface proteins and osteoblast markers: characterization of human adipose tissue-derived osteogenic cells.

European journal of trauma and emergency surgery : official publication of the European Trauma Society

O A Trentz, D Arikketh, V Sentilnathan, S Hemmi, A E Handschin, B de Rosario, P Mohandas, P V A Mohandas

Affiliations

  1. MIR (MIOT Institute of Research), MIOT Hospitals, Chennai, India. [email protected].
  2. , Klusweg 18, 8032, Zurich, Switzerland. [email protected].
  3. MIR (MIOT Institute of Research), MIOT Hospitals, Chennai, India.
  4. Institute of Clinical Research, University Hospital Zurich, Zurich, Switzerland.

PMID: 26816227 DOI: 10.1007/s00068-010-0030-0

Abstract

INTRODUCTION: In adipose tissue-derived osteogenic cells (ADOC), osteoblast markers and surface proteins were determined and compared with osteoblasts harvested from cancellous bone (OB).

METHOD: Osteocalcin (OC), core binding factor 1 (CBFA1), collagen type 1 (Coll1), alkaline phosphatase (ALP), nucleostemin (NS), and surface proteins CD 10, CD44, CD 59 and CD 105 were analyzed using RT-PCR, immunofluorescence and Western blot.

RESULTS: Osteocalcin expression was more distinct in OB than in ADOC, but the other markers and surface proteins showed no differences.

CONCLUSION: These data support the use of adipose tissue for future regenerative medicine; however, further studies are necessary to establish the role of long-term differentiation.

Keywords: Adipose tissue-derived osteogenic cells; Osteoblast markers; Tissue engineering

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