Display options
Cite
Schumacher NS, Colomeu TC, de Figueiredo D, et al. Identification and Antioxidant Activity of the Extracts of Eugenia uniflora Leaves. Characterization of the Anti-Inflammatory Properties of Aqueous Extract on Diabetes Expression in an Experimental Model of Spontaneous Type 1 Diabetes (NOD Mice). Antioxidants (Basel). 2015;4(4):662-80doi: 10.3390/antiox4040662.
Schumacher, N. S., Colomeu, T. C., de Figueiredo, D., Carvalho, V. d. e. . C., Cazarin, C. B., Prado, M. A., Meletti, L. M., & Zollner, R. d. e. . L. (2015). Identification and Antioxidant Activity of the Extracts of Eugenia uniflora Leaves. Characterization of the Anti-Inflammatory Properties of Aqueous Extract on Diabetes Expression in an Experimental Model of Spontaneous Type 1 Diabetes (NOD Mice). Antioxidants (Basel, Switzerland), 4(4), 662-80. https://doi.org/10.3390/antiox4040662
Schumacher, Nayara Simon Gonzalez, et al. "Identification and Antioxidant Activity of the Extracts of Eugenia uniflora Leaves. Characterization of the Anti-Inflammatory Properties of Aqueous Extract on Diabetes Expression in an Experimental Model of Spontaneous Type 1 Diabetes (NOD Mice)." Antioxidants (Basel, Switzerland) vol. 4,4 (2015): 662-80. doi: https://doi.org/10.3390/antiox4040662
Schumacher NS, Colomeu TC, de Figueiredo D, Carvalho Vde C, Cazarin CB, Prado MA, Meletti LM, Zollner Rde L. Identification and Antioxidant Activity of the Extracts of Eugenia uniflora Leaves. Characterization of the Anti-Inflammatory Properties of Aqueous Extract on Diabetes Expression in an Experimental Model of Spontaneous Type 1 Diabetes (NOD Mice). Antioxidants (Basel). 2015 Oct 09;4(4):662-80. doi: 10.3390/antiox4040662. PMID: 26783951; PMCID: PMC4712943.
Copy Download .nbib Format: NLM
Share it on

Antioxidants (Basel). 2015 Oct 09;4(4):662-80. doi: 10.3390/antiox4040662.

Identification and Antioxidant Activity of the Extracts of Eugenia uniflora Leaves. Characterization of the Anti-Inflammatory Properties of Aqueous Extract on Diabetes Expression in an Experimental Model of Spontaneous Type 1 Diabetes (NOD Mice).

Antioxidants (Basel, Switzerland)

Nayara Simon Gonzalez Schumacher, Talita Cristina Colomeu, Daniella de Figueiredo, Virginia de Campos Carvalho, Cinthia Baú Betim Cazarin, Marcelo Alexandre Prado, Laura Maria Molina Meletti, Ricardo de Lima Zollner

Affiliations

  1. Laboratory of Translational Immunology, Faculty of Medical Sciences, University of Campinas, Rua Vital Brazil, 300, Cidade Universitária Zeferino Vaz, 13083-888 Campinas-SP, Brazil. [email protected].
  2. Laboratory of Translational Immunology, Faculty of Medical Sciences, University of Campinas, Rua Vital Brazil, 300, Cidade Universitária Zeferino Vaz, 13083-888 Campinas-SP, Brazil. [email protected].
  3. Laboratory of Translational Immunology, Faculty of Medical Sciences, University of Campinas, Rua Vital Brazil, 300, Cidade Universitária Zeferino Vaz, 13083-888 Campinas-SP, Brazil.
  4. Laboratory of Translational Immunology, Faculty of Medical Sciences, University of Campinas, Rua Vital Brazil, 300, Cidade Universitária Zeferino Vaz, 13083-888 Campinas-SP, Brazil. [email protected].
  5. Department of Food and Nutrition, School of Food Engineering, University of Campinas (FEA-Unicamp), Rua Monteiro Lobato, 80, Cidade Universitária Zeferino Vaz, 13083-862 Campinas-SP, Brazil. [email protected].
  6. Department of Food and Nutrition, School of Food Engineering, University of Campinas (FEA-Unicamp), Rua Monteiro Lobato, 80, Cidade Universitária Zeferino Vaz, 13083-862 Campinas-SP, Brazil. [email protected].
  7. Agronomic Institute of Campinas, Theodureto de Almeida Camargo, 1500, Vila Nova, 13012-970 Campinas-SP, Brazil. [email protected].
  8. Laboratory of Translational Immunology, Faculty of Medical Sciences, University of Campinas, Rua Vital Brazil, 300, Cidade Universitária Zeferino Vaz, 13083-888 Campinas-SP, Brazil. [email protected].
  9. Department of Internal Medicine, Faculty of Medical Sciences, University of Campinas, Rua Tessália Vieira de Camargo, 126, Cidade Universitária Zeferino Vaz, 13083-887 Campinas-SP, Brazil. [email protected].

PMID: 26783951 PMCID: PMC4712943 DOI: 10.3390/antiox4040662

Abstract

Medical and folklore reports suggest that Eugenia uniflora (E. uniflora) is a functional food that contains numerous compounds in its composition, with anti-inflammatory, antioxidant and anti-diabetic effects. In the present study, we investigated the best solvents (water, ethanol and methanol/acetone) for extracting bioactive compounds of E. uniflora leaves, assessing total phenols and the antioxidant activity of the extracts by 2,2-Diphenyl-1-picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP), 2,2'-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and Oxygen Radical Absorbance Capacity (ORAC) assays, identifying hydrolysable tannins and three phenolic compounds (ellagic acid, gallic acid and rutin) present in the leaves. In addition, we evaluated the incidence of diabetes, degree of insulitis, serum insulin, hepatic glutathione and tolerance test glucose in non-obese diabetic (NOD) mice. Our results suggest that the aqueous extract presents antioxidant activity and high total phenols, which were used as a type 1 diabetes mellitus (DM-1) treatment in NOD mice. We verified that the chronic consumption of aqueous extract reduces the inflammatory infiltrate index in pancreatic islets, maintaining serum insulin levels and hepatic glutathione, and reducing serum lipid peroxidation as well as the risk for diabetes.

Keywords: Eugenia uniflora; NOD mice; antioxidant; type 1 diabetes mellitus

References

  1. Anal Biochem. 1972 Aug;48(2):422-7 - PubMed
  2. Indian J Clin Biochem. 2009 Oct;24(4):419-25 - PubMed
  3. Food Chem. 2014 Feb 15;145:522-9 - PubMed
  4. Nutrition. 2002 Jan;18(1):75-81 - PubMed
  5. J Biochem Mol Toxicol. 2003;17(1):24-38 - PubMed
  6. Cell Mol Biol (Noisy-le-grand). 2007 May 30;53(5):68-78 - PubMed
  7. Diabetologia. 1989 May;32(5):282-9 - PubMed
  8. Diabetes Care. 2011 Jan;34(1):162-7 - PubMed
  9. Jikken Dobutsu. 1980 Jan;29(1):1-13 - PubMed
  10. Am J Physiol Endocrinol Metab. 2008 Dec;295(6):E1323-32 - PubMed
  11. Curr Protoc Immunol. 2001 May;Chapter 15:Unit 15.9 - PubMed
  12. Diabetes. 2014 Nov;63(11):3856-67 - PubMed
  13. J Agric Food Chem. 2000 Aug;48(8):3396-402 - PubMed
  14. Acta Diabetol. 2002 Sep;39(3):117-22 - PubMed
  15. Food Chem Toxicol. 1995 Jul;33(7):601-17 - PubMed
  16. J Nutr. 1992 Mar;122(3):442-9 - PubMed
  17. Plant Cell Environ. 2010 Aug 1;33(8):1239-47 - PubMed
  18. Braz J Med Biol Res. 2002 Nov;35(11):1347-55 - PubMed
  19. Diabetes. 1997 May;46(5):907-11 - PubMed
  20. Int Immunopharmacol. 2014 Jan;18(1):106-15 - PubMed
  21. Clin Chim Acta. 2002 Jul;321(1-2):89-96 - PubMed
  22. J Agric Food Chem. 2004 Jul 28;52(15):4669-74 - PubMed
  23. Anal Biochem. 1979 Jun;95(2):351-8 - PubMed
  24. Anal Biochem. 1996 Jul 15;239(1):70-6 - PubMed
  25. J Biochem Mol Toxicol. 2006;20(2):96-102 - PubMed
  26. J Agric Food Chem. 2003 Apr 9;51(8):2144-55 - PubMed
  27. J Agric Food Chem. 2003 May 21;51(11):3273-9 - PubMed
  28. Pol Arch Med Wewn. 2014;124(11):599-607 - PubMed
  29. Pak J Med Sci. 2013 Jul;29(4):938-42 - PubMed
  30. Cytokine. 2008 Apr;42(1):92-104 - PubMed
  31. J Agric Food Chem. 2001 Dec;49(12):5797-808 - PubMed
  32. Annu Rev Immunol. 2005;23:447-85 - PubMed

Publication Types