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Ting TX, Hashim R, Ahmad N, et al. Detection of Bordetella pertussis from Clinical Samples by Culture and End-Point PCR in Malaysian Patients. Int J Bacteriol. 2013;2013:324136doi: 10.1155/2013/324136.
Ting, T. X., Hashim, R., Ahmad, N., & Abdullah, K. H. (2013). Detection of Bordetella pertussis from Clinical Samples by Culture and End-Point PCR in Malaysian Patients. International journal of bacteriology, 2013324136. https://doi.org/10.1155/2013/324136
Ting, Tan Xue, et al. "Detection of Bordetella pertussis from Clinical Samples by Culture and End-Point PCR in Malaysian Patients." International journal of bacteriology vol. 2013 (2013): 324136. doi: https://doi.org/10.1155/2013/324136
Ting TX, Hashim R, Ahmad N, Abdullah KH. Detection of Bordetella pertussis from Clinical Samples by Culture and End-Point PCR in Malaysian Patients. Int J Bacteriol. 2013;2013:324136. doi: 10.1155/2013/324136. Epub 2013 May 07. PMID: 26904725; PMCID: PMC4745474.
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Int J Bacteriol. 2013;2013:324136. doi: 10.1155/2013/324136. Epub 2013 May 07.

Detection of Bordetella pertussis from Clinical Samples by Culture and End-Point PCR in Malaysian Patients.

International journal of bacteriology

Tan Xue Ting, Rohaidah Hashim, Norazah Ahmad, Khairul Hafizi Abdullah

Affiliations

  1. Bacteriology Unit, Infectious Disease Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia.

PMID: 26904725 PMCID: PMC4745474 DOI: 10.1155/2013/324136

Abstract

Pertussis or whooping cough is a highly infectious respiratory disease caused by Bordetella pertussis. In vaccinating countries, infants, adolescents, and adults are relevant patients groups. A total of 707 clinical specimens were received from major hospitals in Malaysia in year 2011. These specimens were cultured on Regan-Lowe charcoal agar and subjected to end-point PCR, which amplified the repetitive insertion sequence IS481 and pertussis toxin promoter gene. Out of these specimens, 275 were positive: 4 by culture only, 6 by both end-point PCR and culture, and 265 by end-point PCR only. The majority of the positive cases were from ≤3 months old patients (77.1%) (P < 0.001). There was no significant association between type of samples collected and end-point PCR results (P > 0.05). Our study showed that the end-point PCR technique was able to pick up more positive cases compared to culture method.

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