Display options
Cite
Panja C, Setty RK, Vaidyanathan G, et al. Label-Free Proteomic Analysis of Flavohemoglobin Deleted Strain of Saccharomyces cerevisiae. Int J Proteomics. 2016;2016:8302423doi: 10.1155/2016/8302423.
Panja, C., Setty, R. K., Vaidyanathan, G., & Ghosh, S. (2016). Label-Free Proteomic Analysis of Flavohemoglobin Deleted Strain of Saccharomyces cerevisiae. International journal of proteomics, 20168302423. https://doi.org/10.1155/2016/8302423
Panja, Chiranjit, et al. "Label-Free Proteomic Analysis of Flavohemoglobin Deleted Strain of Saccharomyces cerevisiae." International journal of proteomics vol. 2016 (2016): 8302423. doi: https://doi.org/10.1155/2016/8302423
Panja C, Setty RK, Vaidyanathan G, Ghosh S. Label-Free Proteomic Analysis of Flavohemoglobin Deleted Strain of Saccharomyces cerevisiae. Int J Proteomics. 2016;2016:8302423. doi: 10.1155/2016/8302423. Epub 2016 Jan 11. PMID: 26881076; PMCID: PMC4737026.
Copy Download .nbib Format: NLM
Share it on

Int J Proteomics. 2016;2016:8302423. doi: 10.1155/2016/8302423. Epub 2016 Jan 11.

Label-Free Proteomic Analysis of Flavohemoglobin Deleted Strain of Saccharomyces cerevisiae.

International journal of proteomics

Chiranjit Panja, Rakesh K S Setty, Gopal Vaidyanathan, Sanjay Ghosh

Affiliations

  1. Department of Biochemistry, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, West Bengal 700 019, India.
  2. Waters India Pvt. Ltd., Bangalore 560 058, India.

PMID: 26881076 PMCID: PMC4737026 DOI: 10.1155/2016/8302423

Abstract

Yeast flavohemoglobin, YHb, encoded by the nuclear gene YHB1, has been implicated in the nitrosative stress responses in Saccharomyces cerevisiae. It is still unclear how S. cerevisiae can withstand this NO level in the absence of flavohemoglobin. To better understand the physiological function of flavohemoglobin in yeast, in the present study a label-free differential proteomics study has been carried out in wild-type and YHB1 deleted strains of S. cerevisiae grown under fermentative conditions. From the analysis, 417 proteins in Y190 and 392 proteins in ΔYHB1 were identified with high confidence. Interestingly, among the differentially expressed identified proteins, 40 proteins were found to be downregulated whereas 41 were found to be upregulated in ΔYHB1 strain of S. cerevisiae (p value < 0.05). The differentially expressed proteins were also classified according to gene ontology (GO) terms. The most enriched and significant GO terms included nitrogen compound biosynthesis, amino acid biosynthesis, translational regulation, and protein folding. Interactions of differentially expressed proteins were generated using Search Tool for the Retrieval of Interacting Genes (STRING) database. This is the first report which offers a more complete view of the proteome changes in S. cerevisiae in the absence of flavohemoglobin.

References

  1. FEBS Lett. 1999 Feb 26;445(2-3):389-94 - PubMed
  2. Nat Prod Rep. 2000 Feb;17(1):85-97 - PubMed
  3. Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4672-6 - PubMed
  4. Mol Microbiol. 2000 May;36(4):775-83 - PubMed
  5. RNA. 2001 Mar;7(3):382-94 - PubMed
  6. Infect Immun. 2002 Aug;70(8):4399-405 - PubMed
  7. Biochem Biophys Res Commun. 2003 Sep 12;309(1):217-21 - PubMed
  8. Biochem J. 1954 May 15;57(329th Meeting):xxix-xxx - PubMed
  9. J Biol Chem. 2005 Mar 4;280(9):7645-53 - PubMed
  10. Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):5015-9 - PubMed
  11. Mol Biol Cell. 2005 Oct;16(10):4814-26 - PubMed
  12. Bioinformatics. 2005 Dec 15;21(24):4432-3 - PubMed
  13. Mol Cell Proteomics. 2006 Jan;5(1):144-56 - PubMed
  14. Eukaryot Cell. 2006 Mar;5(3):518-29 - PubMed
  15. Yeast. 2006 May;23(7):519-35 - PubMed
  16. BMC Bioinformatics. 2006 Jun 15;7:302 - PubMed
  17. J Biol Chem. 2006 Sep 22;281(38):28039-47 - PubMed
  18. Redox Rep. 2006;11(5):231-9 - PubMed
  19. Proc Natl Acad Sci U S A. 2007 May 15;104(20):8484-9 - PubMed
  20. Biochem J. 2009 Feb 1;417(3):783-9 - PubMed
  21. Nucleic Acids Res. 2009 Jan;37(1):1-13 - PubMed
  22. Nat Protoc. 2009;4(1):44-57 - PubMed
  23. Biochem Biophys Res Commun. 2009 Aug 7;385(4):507-11 - PubMed
  24. Mol Cell Proteomics. 2009 Nov;8(11):2443-60 - PubMed
  25. Biochem Biophys Res Commun. 2009 Oct 23;388(3):612-7 - PubMed
  26. Proc Natl Acad Sci U S A. 2009 Sep 8;106(36):15356-61 - PubMed
  27. Arch Biochem Biophys. 2010 Apr 15;496(2):109-16 - PubMed
  28. Eukaryot Cell. 2010 Jun;9(6):971-80 - PubMed
  29. Nat Rev Mol Cell Biol. 2011 Apr;12(4):235-45 - PubMed
  30. Cell Host Microbe. 2011 Jul 21;10(1):33-43 - PubMed
  31. PLoS One. 2011;6(7):e21800 - PubMed
  32. Mol Microbiol. 2011 Nov;82(3):578-90 - PubMed
  33. Arch Biochem Biophys. 2011 Dec 1;516(1):67-74 - PubMed
  34. J Biol Chem. 2012 Dec 21;287(52):43585-98 - PubMed
  35. Nucleic Acids Res. 2013 Jan;41(Database issue):D808-15 - PubMed
  36. Cell Death Dis. 2013 Feb 14;4:e491 - PubMed
  37. FEBS J. 2013 Nov;280(22):5737-49 - PubMed
  38. Biochem Biophys Res Commun. 2014 Sep 5;451(4):529-34 - PubMed
  39. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2286-90 - PubMed
  40. Crit Rev Microbiol. 1985;12(2):131-51 - PubMed
  41. J Biol Chem. 1995 Mar 24;270(12):6991-6 - PubMed
  42. J Bacteriol. 1996 Jul;178(13):3803-8 - PubMed
  43. J Bacteriol. 1996 Sep;178(18):5487-92 - PubMed
  44. J Biol Chem. 1996 Oct 11;271(41):25131-8 - PubMed
  45. Anal Biochem. 1976 May 7;72:248-54 - PubMed
  46. J Exp Biol. 1998 Apr;201(Pt 8):1099-117 - PubMed
  47. J Biol Chem. 1998 Apr 17;273(16):9527-33 - PubMed
  48. J Biol Chem. 1998 May 15;273(20):12543-7 - PubMed
  49. Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10378-83 - PubMed
  50. Mol Microbiol. 1998 Aug;29(4):1101-12 - PubMed
  51. Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14100-5 - PubMed
  52. J Biol Chem. 1999 Jan 8;274(2):748-54 - PubMed

Publication Types