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Tsubokawa D, Sugiyama H, Mikami F, et al. Collection methods of trematode eggs using experimental animal models. Parasitol Int. 2016;65(5):584-587doi: 10.1016/j.parint.2016.01.006.
Tsubokawa, D., Sugiyama, H., Mikami, F., Shibata, K., Shibahara, T., Fukuda, K., Takamiya, S., Yamasaki, H., Nakamura, T., & Tsuji, N. (2016). Collection methods of trematode eggs using experimental animal models. Parasitology international, 65(5), 584-587. https://doi.org/10.1016/j.parint.2016.01.006
Tsubokawa, Daigo, et al. "Collection methods of trematode eggs using experimental animal models." Parasitology international vol. 65,5 (2016): 584-587. doi: https://doi.org/10.1016/j.parint.2016.01.006
Tsubokawa D, Sugiyama H, Mikami F, Shibata K, Shibahara T, Fukuda K, Takamiya S, Yamasaki H, Nakamura T, Tsuji N. Collection methods of trematode eggs using experimental animal models. Parasitol Int. 2016 Oct;65(5):584-587. doi: 10.1016/j.parint.2016.01.006. Epub 2016 Jan 12. PMID: 26792074.
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Parasitol Int. 2016 Oct;65(5):584-587. doi: 10.1016/j.parint.2016.01.006. Epub 2016 Jan 12.

Collection methods of trematode eggs using experimental animal models.

Parasitology international

Daigo Tsubokawa, Hiromu Sugiyama, Fusako Mikami, Katsumasa Shibata, Toshiyuki Shibahara, Koichi Fukuda, Shinzaburo Takamiya, Hiroshi Yamasaki, Takeshi Nakamura, Naotoshi Tsuji

Affiliations

  1. Department of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami, Sagamihara, Kanagawa 252-0374, Japan.
  2. Department of Parasitology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
  3. Department of Animal Risk Management, Faculty of Risk and Crisis Management, Chiba Institute of Science, 15-8 Shiomi, Choshi, Chiba 288-0025, Japan.
  4. Center for Laboratory Animal Science, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
  5. Department of Molecular and Cellular Parasitology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
  6. Department of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami, Sagamihara, Kanagawa 252-0374, Japan. Electronic address: [email protected].

PMID: 26792074 DOI: 10.1016/j.parint.2016.01.006

Abstract

Although observing the eggs of human parasitic helminth is essential for medical education in parasitology, opportunities for collection of the eggs are limited. Collection of the eggs using experimental animal models is needed for a sustainable supply. The metacercariae of three trematode species, Paragonimus westermani, Clonorchis sinensis and Metagonimus yokogawai, were collected from the second intermediate hosts: freshwater crabs and fishes, which were obtained using online shopping in Japan, and inoculated to experimental animal rat and dog. Consequently, eggs of the three trematode species were obtained abundantly from the feces of the animals. The eggs are being used for student training in several Japanese universities. In this article, we introduce the collection procedures for trematode eggs.

Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Keywords: Clonorchis sinensis; Metacercaria; Metagonimus yokogawai; Paragonimus westermani; Trematode eggs

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