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Goltermann L, Sarusie MV, Bentin T. Chaperonin GroEL/GroES Over-Expression Promotes Aminoglycoside Resistance and Reduces Drug Susceptibilities in Escherichia coli Following Exposure to Sublethal Aminoglycoside Doses. Front Microbiol. 2016;6:1572doi: 10.3389/fmicb.2015.01572.
Goltermann, L., Sarusie, M. V., & Bentin, T. (2015). Chaperonin GroEL/GroES Over-Expression Promotes Aminoglycoside Resistance and Reduces Drug Susceptibilities in Escherichia coli Following Exposure to Sublethal Aminoglycoside Doses. Frontiers in microbiology, 61572. https://doi.org/10.3389/fmicb.2015.01572
Goltermann, Lise, et al. "Chaperonin GroEL/GroES Over-Expression Promotes Aminoglycoside Resistance and Reduces Drug Susceptibilities in Escherichia coli Following Exposure to Sublethal Aminoglycoside Doses." Frontiers in microbiology vol. 6 (2015): 1572. doi: https://doi.org/10.3389/fmicb.2015.01572
Goltermann L, Sarusie MV, Bentin T. Chaperonin GroEL/GroES Over-Expression Promotes Aminoglycoside Resistance and Reduces Drug Susceptibilities in Escherichia coli Following Exposure to Sublethal Aminoglycoside Doses. Front Microbiol. 2016 Jan 26;6:1572. doi: 10.3389/fmicb.2015.01572. eCollection 2015. PMID: 26858694; PMCID: PMC4726795.
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Front Microbiol. 2016 Jan 26;6:1572. doi: 10.3389/fmicb.2015.01572. eCollection 2015.

Chaperonin GroEL/GroES Over-Expression Promotes Aminoglycoside Resistance and Reduces Drug Susceptibilities in Escherichia coli Following Exposure to Sublethal Aminoglycoside Doses.

Frontiers in microbiology

Lise Goltermann, Menachem V Sarusie, Thomas Bentin

Affiliations

  1. Department of Cellular and Molecular Medicine, University of Copenhagen Copenhagen, Denmark.

PMID: 26858694 PMCID: PMC4726795 DOI: 10.3389/fmicb.2015.01572

Abstract

Antibiotic resistance is an increasing challenge to modern healthcare. Aminoglycoside antibiotics cause translation corruption and protein misfolding and aggregation in Escherichia coli. We previously showed that chaperonin GroEL/GroES depletion and over-expression sensitize and promote short-term tolerance, respectively, to this drug class. Here, we show that chaperonin GroEL/GroES over-expression accelerates acquisition of streptomycin resistance and reduces susceptibility to several other antibiotics following sub-lethal streptomycin antibiotic exposure. Chaperonin buffering could provide a novel mechanism for emergence of antibiotic resistance.

Keywords: aminoglycosides; antibiotic resistance; chaperonin; mRNA translation; protein misfolding

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