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Beuker J, Steier A, Wittgens A, et al. Integrated foam fractionation for heterologous rhamnolipid production with recombinant Pseudomonas putida in a bioreactor. AMB Express. 2016;6(1):11doi: 10.1186/s13568-016-0183-2.
Beuker, J., Steier, A., Wittgens, A., Rosenau, F., Henkel, M., & Hausmann, R. (2016). Integrated foam fractionation for heterologous rhamnolipid production with recombinant Pseudomonas putida in a bioreactor. AMB Express, 6(1), 11. https://doi.org/10.1186/s13568-016-0183-2
Beuker, Janina, et al. "Integrated foam fractionation for heterologous rhamnolipid production with recombinant Pseudomonas putida in a bioreactor." AMB Express vol. 6,1 (2016): 11. doi: https://doi.org/10.1186/s13568-016-0183-2
Beuker J, Steier A, Wittgens A, Rosenau F, Henkel M, Hausmann R. Integrated foam fractionation for heterologous rhamnolipid production with recombinant Pseudomonas putida in a bioreactor. AMB Express. 2016 Mar;6(1):11. doi: 10.1186/s13568-016-0183-2. Epub 2016 Feb 09. PMID: 26860613; PMCID: PMC4747948.
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AMB Express. 2016 Mar;6(1):11. doi: 10.1186/s13568-016-0183-2. Epub 2016 Feb 09.

Integrated foam fractionation for heterologous rhamnolipid production with recombinant Pseudomonas putida in a bioreactor.

AMB Express

Janina Beuker, Anke Steier, Andreas Wittgens, Frank Rosenau, Marius Henkel, Rudolf Hausmann

Affiliations

  1. Institute of Food Science and Biotechnology, Department of Bioprocess Engineering (150k), University of Hohenheim, Fruwirthstr. 12, 70599, Stuttgart, Germany.
  2. Center for Peptide Pharmaceuticals, Ulm University, Meyerhofstr. 1, 89081, Ulm, Germany.
  3. Institute of Food Science and Biotechnology, Department of Bioprocess Engineering (150k), University of Hohenheim, Fruwirthstr. 12, 70599, Stuttgart, Germany. [email protected].

PMID: 26860613 PMCID: PMC4747948 DOI: 10.1186/s13568-016-0183-2

Abstract

Heterologeous production of rhamnolipids in Pseudomonas putida is characterized by advantages of a non-pathogenic host and avoidance of the native quorum sensing regulation in Pseudomonas aeruginosa. Yet, downstream processing is a major problem in rhamnolipid production and increases in complexity at low rhamnolipid titers and when using chemical foam control. This leaves the necessity of a simple concentrating and purification method. Foam fractionation is an elegant method for in situ product removal when producing microbial surfactants. However, up to now in situ foam fractionation is nearly exclusively reported for the production of surfactin with Bacillus subtilis. So far no cultivation integrated foam fractionation process for rhamnolipid production has been reported. This is probably due to excessive bacterial foam enrichment in that system. In this article a simple integrated foam fractionation process is reported for heterologous rhamnolipid production in a bioreactor with easily manageable bacterial foam enrichments. Rhamnolipids were highly concentrated in the foam during the cultivation process with enrichment factors up to 200. The described process was evaluated at different pH, media compositions and temperatures. Foam fractionation processes were characterized by calculating procedural parameter including rhamnolipid and bacterial enrichment, rhamnolipid recovery, YX/S, YP/X, and specific as well as volumetric productivities. Comparing foam fractionation parameters of the rhamnolipid process with the surfactin process a high effectiveness of the integrated foam fractionation for rhamnolipid production was demonstrated.

Keywords: Biosurfactant; Downstream processing; Foam fractionation; Heterologous rhamnolipid; In situ product removal (ISPR); Pseudomonas putida

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