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Bronkhorst AJ, Aucamp J, Pretorius PJ. Adjustments to the preanalytical phase of quantitative cell-free DNA analysis. Data Brief. 2015;6:326-9doi: 10.1016/j.dib.2015.12.009.
Bronkhorst, A. J., Aucamp, J., & Pretorius, P. J. (2016). Adjustments to the preanalytical phase of quantitative cell-free DNA analysis. Data in brief, 6326-9. https://doi.org/10.1016/j.dib.2015.12.009
Bronkhorst, Abel Jacobus, et al. "Adjustments to the preanalytical phase of quantitative cell-free DNA analysis." Data in brief vol. 6 (2016): 326-9. doi: https://doi.org/10.1016/j.dib.2015.12.009
Bronkhorst AJ, Aucamp J, Pretorius PJ. Adjustments to the preanalytical phase of quantitative cell-free DNA analysis. Data Brief. 2015 Dec 17;6:326-9. doi: 10.1016/j.dib.2015.12.009. eCollection 2016 Mar. PMID: 26862578; PMCID: PMC4706619.
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Data Brief. 2015 Dec 17;6:326-9. doi: 10.1016/j.dib.2015.12.009. eCollection 2016 Mar.

Adjustments to the preanalytical phase of quantitative cell-free DNA analysis.

Data in brief

Abel Jacobus Bronkhorst, Janine Aucamp, Piet J Pretorius

Affiliations

  1. Centre for Human Metabolomics, Biochemistry Division, North-West University, Potchefstroom 2520, South Africa.

PMID: 26862578 PMCID: PMC4706619 DOI: 10.1016/j.dib.2015.12.009

Abstract

Evaluating the kinetics of cell-free DNA (cfDNA) in the blood of cancer patients could be a strong auxiliary component to the molecular characterization of cfDNA, but its potential clinical significance is obscured by the absence of an analytical consensus. To utilize quantitative cfDNA assessment with confidence, it is crucial that the preanalytical phase is standardized. In a previous publication, several preanalytical variables that may affect quantitative measurements of cfDNA were identified, and the most confounding variables were assessed further using the growth medium of cultured cancer cells as a source of cfDNA ("Cell-free DNA: Preanalytical variables" [1]). The data accompanying this report relates to these experiments, which includes numerous changes to the sample handling and isolation protocols, and can be used for the interpretation of these results and other similar experiments by different researchers.

References

  1. Clin Chim Acta. 2015 Oct 23;450:243-53 - PubMed

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