Display options
Cite
Sun Y, Li F, Fan C. Effect of pERK2 on extracellular matrix turnover of the fibrotic joint capsule in a post-traumatic joint contracture model. Exp Ther Med. 2015;11(2):547-552doi: 10.3892/etm.2015.2948.
Sun, Y., Li, F., & Fan, C. (2016). Effect of pERK2 on extracellular matrix turnover of the fibrotic joint capsule in a post-traumatic joint contracture model. Experimental and therapeutic medicine, 11(2), 547-552. https://doi.org/10.3892/etm.2015.2948
Sun, Yangbai, et al. "Effect of pERK2 on extracellular matrix turnover of the fibrotic joint capsule in a post-traumatic joint contracture model." Experimental and therapeutic medicine vol. 11,2 (2016): 547-552. doi: https://doi.org/10.3892/etm.2015.2948
Sun Y, Li F, Fan C. Effect of pERK2 on extracellular matrix turnover of the fibrotic joint capsule in a post-traumatic joint contracture model. Exp Ther Med. 2016 Feb;11(2):547-552. doi: 10.3892/etm.2015.2948. Epub 2015 Dec 16. PMID: 26893645; PMCID: PMC4734203.
Copy Download .nbib Format: NLM
Share it on

Exp Ther Med. 2016 Feb;11(2):547-552. doi: 10.3892/etm.2015.2948. Epub 2015 Dec 16.

Effect of pERK2 on extracellular matrix turnover of the fibrotic joint capsule in a post-traumatic joint contracture model.

Experimental and therapeutic medicine

Yangbai Sun, Fengfeng Li, Cunyi Fan

Affiliations

  1. Department of Orthopedics, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China.

PMID: 26893645 PMCID: PMC4734203 DOI: 10.3892/etm.2015.2948

Abstract

Lentivirus (LV)-mediated extracellular signal-regulated kinase (ERK)2 small interfering RNA (siRNA) has previously been demonstrated to reduce post-traumatic joint contractures: In the present study, the effect of ERK2 siRNA on extracellular matrix turnover within fibrotic joint capsules in post-traumatic joint contractures was examined. Rats were randomly assigned to one of three groups as follows: The non-operated control (CON), operated contracture (ORC) and contracture-treatment (CNT) groups. Representative post-traumatic joint contracture was created through 8 weeks of immobilization following intra-articular injury. In the CNT group, LV-mediated ERK2 siRNA was injected into the model knee at days 3 and 7 after surgery. The posterior joint capsule was examined by western blotting, histology and immunohistochemistry to evaluate alterations in ERK2, phosphorylated (p)ERK2, total collagen, collagen I, collagen III, matrix metalloproteinase (MMP)-1, MMP-13 and tissue inhibitor of metalloproteinase (TIMP)-13. In the ORC group, pERK2 was elevated and total collagen, collagen I, MMP-1 and MMP-13 were significantly increased (P<0.01 vs. CON group); however, these were significantly decreased in the CNT group, and pERK2 was downregulated (P<0.01 vs. ORC group). Collagen III and TIMP-13 were markedly decreased in the ORC group (P<0.01 vs. CON group), but elevated in the CNT group (P<0.01 vs. ORC group). The present res4ults demonstrate unique pathological changes of the fibrotic joint capsule that are responsible for joint contracture following traumatic injury, and reveal that extracellular matrix turnover can be affected by pERK2.

Keywords: collagen; joint contracture; matrix metalloproteinase-1; matrix metalloproteinase-13; tissue inhibitor of metalloproteinase-13

References

  1. Clin Oral Investig. 2002 Sep;6(3):175-82 - PubMed
  2. J Shoulder Elbow Surg. 2007 Jul-Aug;16(4):484-90 - PubMed
  3. Acta Orthop Scand. 2002 Jun;73(3):335-43 - PubMed
  4. Clin Orthop Relat Res. 2004 Feb;(419):189-97 - PubMed
  5. J Orthop Res. 2012 May;30(5):713-9 - PubMed
  6. Clin Orthop Relat Res. 2005 Oct;439:228-34 - PubMed
  7. J Hand Surg Am. 2007 Dec;32(10):1605-23 - PubMed
  8. Clin Orthop Relat Res. 1977 Jan-Feb;(122):359-65 - PubMed
  9. Int J Mol Sci. 2013 Oct 17;14(10):20833-44 - PubMed
  10. J Orthop Res. 2001 May;19(3):456-62 - PubMed
  11. J Orthop Res. 2006 May;24(5):1036-43 - PubMed
  12. Arthroscopy. 1993;9(1):9-13 - PubMed
  13. J Invest Dermatol. 2003 Aug;121(2):267-72 - PubMed
  14. Clin Orthop Relat Res. 1996 Feb;(323):310-5 - PubMed
  15. J Bone Joint Surg Am. 2010 Jun;92(6):1468-77 - PubMed
  16. Acta Orthop. 2008 Feb;79(1):116-25 - PubMed
  17. Clin Orthop Relat Res. 2006 Sep;450:219-24 - PubMed
  18. Clin Orthop Relat Res. 1975 Sep;(111):290-8 - PubMed
  19. J Orthop Res. 2004 Mar;22(2):313-20 - PubMed
  20. J Orthop Res. 2000 Mar;18(2):212-9 - PubMed
  21. Inflamm Res. 2012 Apr;61(4):285-92 - PubMed
  22. Clin Orthop Relat Res. 2001 Feb;(383):250-8 - PubMed

Publication Types