Display options
Cite
Penttilä AK, Rouhiainen A, Kylänpää L, et al. Circulating nucleosomes as predictive markers of severe acute pancreatitis. J Intensive Care. 2016;4:14doi: 10.1186/s40560-016-0135-6.
Penttilä, A. K., Rouhiainen, A., Kylänpää, L., Mustonen, H., Puolakkainen, P., Rauvala, H., & Repo, H. (2016). Circulating nucleosomes as predictive markers of severe acute pancreatitis. Journal of intensive care, 414. https://doi.org/10.1186/s40560-016-0135-6
Penttilä, Anne K, et al. "Circulating nucleosomes as predictive markers of severe acute pancreatitis." Journal of intensive care vol. 4 (2016): 14. doi: https://doi.org/10.1186/s40560-016-0135-6
Penttilä AK, Rouhiainen A, Kylänpää L, Mustonen H, Puolakkainen P, Rauvala H, Repo H. Circulating nucleosomes as predictive markers of severe acute pancreatitis. J Intensive Care. 2016 Feb 17;4:14. doi: 10.1186/s40560-016-0135-6. eCollection 2016. PMID: 26893906; PMCID: PMC4758106.
Copy Download .nbib Format: NLM
Share it on

J Intensive Care. 2016 Feb 17;4:14. doi: 10.1186/s40560-016-0135-6. eCollection 2016.

Circulating nucleosomes as predictive markers of severe acute pancreatitis.

Journal of intensive care

Anne K Penttilä, Ari Rouhiainen, Leena Kylänpää, Harri Mustonen, Pauli Puolakkainen, Heikki Rauvala, Heikki Repo

Affiliations

  1. Department of Surgery, Helsinki University Hospital, P.O. Box 340, 00029 HUS Helsinki, Finland.
  2. Neuroscience Center, University of Helsinki, P.O. Box 56, 00014 Helsinki, Finland.
  3. Department of Biosciences, University of Helsinki, P.O. Box 65, 00014 Helsinki, Finland.
  4. Institute of Clinical Medicine, University of Helsinki, P.O. Box 340, 00029 HUS Helsinki, Finland.
  5. Department of Bacteriology and Immunology, University of Helsinki, The Haartman Institute, P.O. Box 21, 00014 Helsinki, Finland.

PMID: 26893906 PMCID: PMC4758106 DOI: 10.1186/s40560-016-0135-6

Abstract

BACKGROUND: The components of nucleosomes, which contain DNA and histones, are released into the circulation from damaged cells and can promote inflammation. We studied whether the on-admission levels of circulating nucleosomes predict the development of severe acute pancreatitis (AP), in particular among the patients who present without clinical signs of organ dysfunction.

METHODS: This is a prospective study of 74 AP patients admitted to Helsinki University Hospital from 2003 to 2007. Twenty-three patients had mild, 27 moderately severe, and 24 severe AP as defined by the revised Atlanta criteria. 14/24 severe AP patients had no sign of organ dysfunction on admission (modified marshall score <2). Blood samples were obtained on admission and the plasma levels of nucleosomes were measured using enzyme-linked immunosorbent assay.

RESULTS: The on-admission levels of nucleosomes were significantly higher in severe AP than in mild or moderately severe AP (p < 0.001 for all), higher in non-survivors (n = 8) than in survivors (p = 0.019), and correlated with the on-admission levels of C-reactive protein (p < 0.001) and creatinine (p < 0.001). Among the AP patients who presented without organ dysfunction, the on-admission nucleosome level was an independent predictor of severe AP (p = 0.038, gender-adjusted forward-stepping logistic regression).

CONCLUSIONS: Circulating nucleosome levels may be helpful in identifying, on admission to hospital, the AP patients who present without clinical signs of organ dysfunction, and, yet, are bound to develop organ dysfunction during hospitalization.

Keywords: Biomarkers; Cellular damage; Nucleosomes; Organ dysfunction; Pancreatitis

References

  1. World J Gastroenterol. 2006 Dec 21;12(47):7666-70 - PubMed
  2. Cell. 1975 Apr;4(4):281-300 - PubMed
  3. Stat Med. 1998 Apr 30;17(8):857-72 - PubMed
  4. Pancreas. 2012 Apr;41(3):353-7 - PubMed
  5. Arthritis Rheum. 1997 Dec;40(12):2217-25 - PubMed
  6. Crit Care Med. 2014 Dec;42(12):2556-64 - PubMed
  7. J Biol Chem. 2006 Feb 10;281(6):3370-81 - PubMed
  8. Gut. 2001 Jan;48(1):62-9 - PubMed
  9. Clin Exp Immunol. 1992 Oct;90(1):56-62 - PubMed
  10. JOP. 2008 Mar 08;9(2):179-84 - PubMed
  11. J Clin Epidemiol. 2003 Nov;56(11):1129-35 - PubMed
  12. Am J Gastroenterol. 2009 Jan;104(1):164-70 - PubMed
  13. Gastroenterology. 2014 Apr;146(4):1097-107 - PubMed
  14. Crit Care Med. 1995 Oct;23(10):1638-52 - PubMed
  15. Crit Care Med. 2002 Jun;30(6):1274-9 - PubMed
  16. Scand J Gastroenterol. 2001 Oct;36(10):1100-7 - PubMed
  17. Transplant Proc. 2009 Dec;41(10):4207-10 - PubMed
  18. Crit Care Med. 2003 Jul;31(7):1947-51 - PubMed
  19. Pancreatology. 2009;9(4):383-91 - PubMed
  20. Surgery. 2012 Mar;151(3):372-81 - PubMed
  21. Am J Surg. 1993 Sep;166(3):262-8; discussion 269 - PubMed
  22. Gastroenterology. 2011 Jul;141(1):358-69 - PubMed
  23. Int J Infect Dis. 2012 Jul;16(7):e558-64 - PubMed
  24. Pancreas. 2013 May;42(4):640-6 - PubMed
  25. Gut. 1998 Jun;42 Suppl 2:S1-13 - PubMed
  26. Pancreas. 2005 Jul;31(1):23-7 - PubMed
  27. Science. 2004 Mar 5;303(5663):1532-5 - PubMed
  28. Am J Gastroenterol. 2013 Sep;108(9):1400-15; 1416 - PubMed
  29. Gut. 2013 Jan;62(1):102-11 - PubMed
  30. Pancreas. 2011 May;40(4):547-50 - PubMed
  31. Arch Intern Med. 2011 Apr 11;171(7):669-76 - PubMed
  32. Pancreas. 2011 Jul;40(5):787-8 - PubMed
  33. Crit Care Med. 2015 Apr;43(4):e107-16 - PubMed
  34. Ann Surg. 2002 Jan;235(1):68-76 - PubMed
  35. J Immunol. 1996 Feb 1;156(3):1151-6 - PubMed
  36. Br J Surg. 2005 Jan;92(1):68-75 - PubMed
  37. Pancreatology. 2003;3(2):111-4 - PubMed
  38. Anticancer Res. 2010 May;30(5):1613-5 - PubMed
  39. Clin Immunol. 2012 Jul;144(1):32-40 - PubMed
  40. Lancet. 2000 Jun 3;355(9219):1955-60 - PubMed
  41. Br J Surg. 2002 Mar;89(3):298-302 - PubMed
  42. Mol Med. 2014 Oct 29;20:466-77 - PubMed
  43. Ann N Y Acad Sci. 2008 Aug;1137:180-9 - PubMed
  44. Nat Commun. 2015 May 20;6:7151 - PubMed
  45. Gut. 2004 Sep;53(9):1340-4 - PubMed
  46. Nat Med. 2009 Jun;15(6):623-5 - PubMed
  47. Clin Chem. 2003 Apr;49(4):562-9 - PubMed
  48. Crit Care. 2014 May 21;18(3):R104 - PubMed

Publication Types