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da Cruz Ramos GF, Ramos PL, Passarini MRZ, et al. Cellulolytic and proteolytic ability of bacteria isolated from gastrointestinal tract and composting of a hippopotamus. AMB Express. 2016;6(1):17doi: 10.1186/s13568-016-0188-x.
da Cruz Ramos, G. F., Ramos, P. L., Passarini, M. R. Z., Vieira Silveira, M. A., Okamoto, D. N., de Oliveira, L. C. G., Zezzo, L. V., Marem, A., Santos Rocha, R. C., da Cruz, J. B., Juliano, L., & de Vasconcellos, S. P. (2016). Cellulolytic and proteolytic ability of bacteria isolated from gastrointestinal tract and composting of a hippopotamus. AMB Express, 6(1), 17. https://doi.org/10.1186/s13568-016-0188-x
da Cruz Ramos, Geomárcia Feitosa, et al. "Cellulolytic and proteolytic ability of bacteria isolated from gastrointestinal tract and composting of a hippopotamus." AMB Express vol. 6,1 (2016): 17. doi: https://doi.org/10.1186/s13568-016-0188-x
da Cruz Ramos GF, Ramos PL, Passarini MRZ, Vieira Silveira MA, Okamoto DN, de Oliveira LCG, Zezzo LV, Marem A, Santos Rocha RC, da Cruz JB, Juliano L, de Vasconcellos SP. Cellulolytic and proteolytic ability of bacteria isolated from gastrointestinal tract and composting of a hippopotamus. AMB Express. 2016 Mar;6(1):17. doi: 10.1186/s13568-016-0188-x. Epub 2016 Mar 01. PMID: 26931430; PMCID: PMC4773312.
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AMB Express. 2016 Mar;6(1):17. doi: 10.1186/s13568-016-0188-x. Epub 2016 Mar 01.

Cellulolytic and proteolytic ability of bacteria isolated from gastrointestinal tract and composting of a hippopotamus.

AMB Express

Geomárcia Feitosa da Cruz Ramos, Patricia Locosque Ramos, Michel Rodrigo Zambrano Passarini, Marghuel A Vieira Silveira, Débora Noma Okamoto, Lilian Caroline Gonçalves de Oliveira, Larissa Vieira Zezzo, Alyne Marem, Rafael Costa Santos Rocha, João Batista da Cruz, Luiz Juliano, Suzan Pantaroto de Vasconcellos

Affiliations

  1. Department of Biological Sciences, Federal University of São Paulo, Rua São Nicolau, 210, Diadema, São Paulo, 09913-030, Brazil.
  2. São Paulo Zoo Park Foundation, Av. Miguel Estéfano, 4241, São Paulo, São Paulo, 04301-905, Brazil.
  3. Latin American Institute of Life Sciences and Nature, Federal University of Latin American Integration, Av. Tarquínio Joslin dos Santos, 1000, Foz do Iguaçu, Paraná, 85870-901, Brazil.
  4. Department of Biophysics, Federal University of São Paulo, Rua 3 de Maio, 100, São Paulo, São Paulo, 04044-020, Brazil.
  5. Department of Biological Sciences, Federal University of São Paulo, Rua São Nicolau, 210, Diadema, São Paulo, 09913-030, Brazil. [email protected].

PMID: 26931430 PMCID: PMC4773312 DOI: 10.1186/s13568-016-0188-x

Abstract

The bioprospection for cellulase and protease producers is a promise strategy for the discovery of potential biocatalysts for use in hydrolysis of lignocellulosic materials as well as proteic residues. These enzymes can increment and turn viable the production of second generation ethanol from different and alternative sources. In this context, the goal of this study was the investigation of cellulolytic and proteolytic abilities of bacteria isolated from the gastrointestinal tract of a hippopotamus as well as from its composting process. It is important to highlight that hippopotamus gastrointestinal samples were a non-typical sources of efficient hydrolytic bacteria with potential for application in biotechnological industries, like biofuel production. Looking for this, a total of 159 bacteria were isolated, which were submitted to qualitative and quantitative enzymatic assays. Proteolytic analyzes were conducted through the evaluation of fluorescent probes. Qualitative assays for cellulolytic abilities revealed 70 positive hits. After quantitative analyzes, 44 % of these positive hits were selected, but five (5) strains showed cellulolytic activity up to 11,8 FPU/mL. Regarding to proteolytic activities, six (6) strains showed activity above 10 %, which overpassed results described in the literature. Molecular analyzes based on the identification of 16S rDNA, revealed that all the selected bacterial isolates were affiliated to Bacillus genus. In summary, these results strongly indicate that the isolated bacteria from a hippopotamus can be a potential source of interesting biocatalysts with cellulolytic and proteolytic activities, with relevance for industrial applications.

Keywords: Bacillus; Biofuel; Cellulase; Protease; Screening

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