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Li C, Batistel F, Osorio JS, et al. Peripartal rumen-protected methionine supplementation to higher energy diets elicits positive effects on blood neutrophil gene networks, performance and liver lipid content in dairy cows. J Anim Sci Biotechnol. 2016;7:18doi: 10.1186/s40104-016-0077-9.
Li, C., Batistel, F., Osorio, J. S., Drackley, J. K., Luchini, D., & Loor, J. J. (2016). Peripartal rumen-protected methionine supplementation to higher energy diets elicits positive effects on blood neutrophil gene networks, performance and liver lipid content in dairy cows. Journal of animal science and biotechnology, 718. https://doi.org/10.1186/s40104-016-0077-9
Li, Cong, et al. "Peripartal rumen-protected methionine supplementation to higher energy diets elicits positive effects on blood neutrophil gene networks, performance and liver lipid content in dairy cows." Journal of animal science and biotechnology vol. 7 (2016): 18. doi: https://doi.org/10.1186/s40104-016-0077-9
Li C, Batistel F, Osorio JS, Drackley JK, Luchini D, Loor JJ. Peripartal rumen-protected methionine supplementation to higher energy diets elicits positive effects on blood neutrophil gene networks, performance and liver lipid content in dairy cows. J Anim Sci Biotechnol. 2016 Mar 09;7:18. doi: 10.1186/s40104-016-0077-9. eCollection 2016. PMID: 26962451; PMCID: PMC4784469.
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J Anim Sci Biotechnol. 2016 Mar 09;7:18. doi: 10.1186/s40104-016-0077-9. eCollection 2016.

Peripartal rumen-protected methionine supplementation to higher energy diets elicits positive effects on blood neutrophil gene networks, performance and liver lipid content in dairy cows.

Journal of animal science and biotechnology

Cong Li, Fernanda Batistel, Johan Samir Osorio, James K Drackley, Daniel Luchini, Juan J Loor

Affiliations

  1. Key Laboratory of Animal Genetics and Breeding of Ministry of Agriculture, National Engineering Laboratory of Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China.
  2. Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801 USA.
  3. Department of Animal and Rangeland Sciences, Oregon State University, Corvallis, 97331 OR USA.
  4. Ruminant Technical Services, ADISSEO NA, Alpharetta, 30022 GA USA.

PMID: 26962451 PMCID: PMC4784469 DOI: 10.1186/s40104-016-0077-9

Abstract

BACKGROUND: Main objectives were to determine to what extent Smartamine M (SM) supplementation to a prepartal higher-energy diet could alter neutrophil (PMN) and liver tissue immunometabolic biomarkers, and whether those responses were comparable to those in cows fed a prepartal lower-energy diet (CON).

RESULTS: Twenty-eight multiparous Holstein cows were fed CON (NEL = 1.24 Mcal/kg DM) during d -50 to d -22 relative to calving. From d -21 to calving, cows were randomly assigned to a higher-energy diet (OVE, n = 9; NEL = 1.54 Mcal/kg DM), OVE plus SM (OVE + SM, n = 10; SM = 0.07 % of DM) or remained on CON (n = 9). All cows received the same basal lactation diet (NEL = 1.75 Mcal/kg DM). Supplementation of SM (OVE + SM) continued until 30 d postpartum. Liver biopsies were harvested at d -10, 7, and 21 relative to parturition. Blood PMN isolated at -10, 3, and 21 d relative to calving was used to evaluate gene expression. As expected, OVE increased liver lipid content postpartum; however, cows fed OVE + SM or CON had lower concentrations than OVE. Compared with OVE, cows in CON and OVE + SM had greater DMI postpartum and milk production. Furthermore, cows fed OVE + SM had the greatest milk protein and fat percentage and lowest milk SCC despite having intermediate PMN phagocytic capacity. Adaptations in PMN gene expression in OVE + SM cows associated with the lower SCC were gradual increases from -10 to 21 d in genes that facilitate migration into inflammatory sites (SELL, ITGAM), enzymes essential for reducing reactive oxygen metabolites (SOD1, SOD2), and a transcription factor(s) required for controlling PMN development (RXRA). The greater expression of TLR4 on d 3, key for activation of innate immunity due to inflammation, in OVE compared with CON cows suggests a more pronounced inflammatory state. Feeding OVE + SM dampened the upregulation of TLR4, despite the fact that these cows had similar expression of the pro-inflammatory genes NFKB1 and TNF as OVE. Cows in CON had lower overall expression of these inflammation-related genes and GSR, which generates reduced glutathione, an important cellular antioxidant.

CONCLUSIONS: Although CON cows appeared to have a less stressful transition into lactation, SM supplementation was effective in alleviating negative effects of energy-overfeeding. As such, SM was beneficial in terms of production and appeared to boost the response of PMN in a way that improved overall cow health.

Keywords: Blood neutrophil; Gene expression; Methionine

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