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Liu J, Zhou J, Wu Z, et al. Cyanidin 3-O-β-Glucoside Ameliorates Ethanol-Induced Acute Liver Injury by Attenuating Oxidative Stress and Apoptosis: The Role of SIRT1/FOXO1 Signaling. Alcohol Clin Exp Res. 2016;40(3):457-66doi: 10.1111/acer.12982.
Liu, J., Zhou, J., Wu, Z., Wang, X., Liu, L., & Yao, C. (2016). Cyanidin 3-O-β-Glucoside Ameliorates Ethanol-Induced Acute Liver Injury by Attenuating Oxidative Stress and Apoptosis: The Role of SIRT1/FOXO1 Signaling. Alcoholism, clinical and experimental research, 40(3), 457-66. https://doi.org/10.1111/acer.12982
Liu, Juncheng, et al. "Cyanidin 3-O-β-Glucoside Ameliorates Ethanol-Induced Acute Liver Injury by Attenuating Oxidative Stress and Apoptosis: The Role of SIRT1/FOXO1 Signaling." Alcoholism, clinical and experimental research vol. 40,3 (2016): 457-66. doi: https://doi.org/10.1111/acer.12982
Liu J, Zhou J, Wu Z, Wang X, Liu L, Yao C. Cyanidin 3-O-β-Glucoside Ameliorates Ethanol-Induced Acute Liver Injury by Attenuating Oxidative Stress and Apoptosis: The Role of SIRT1/FOXO1 Signaling. Alcohol Clin Exp Res. 2016 Mar;40(3):457-66. doi: 10.1111/acer.12982. PMID: 26934204.
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Alcohol Clin Exp Res. 2016 Mar;40(3):457-66. doi: 10.1111/acer.12982.

Cyanidin 3-O-β-Glucoside Ameliorates Ethanol-Induced Acute Liver Injury by Attenuating Oxidative Stress and Apoptosis: The Role of SIRT1/FOXO1 Signaling.

Alcoholism, clinical and experimental research

Juncheng Liu, Jun Zhou, Zhonghua Wu, Xiaoyu Wang, Liqiong Liu, Chonghua Yao

Affiliations

  1. Clinical Medicine College, Gansu University of Traditional Chinese Medicine, Lanzhou, China.
  2. Center of Minimally Invasive Surgery , Xiangya No. 2 Hospital, Central South University, Changsha, China.
  3. Experiment Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
  4. School of Pharmacy , Shanghai University of Traditional Chinese Medicine, Shanghai, China.
  5. Department of Microbiology and Immunology , Indiana University School of Medicine, Indianapolis, Indiana.
  6. Department of Rheumatism , Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

PMID: 26934204 DOI: 10.1111/acer.12982

Abstract

BACKGROUND: The aim of this study was to examine the effects of Cyanidin 3-O-β-glucoside (C3G) on ethanol (EtOH)-induced acute liver injury in mice as well as in cultured hepatic cells exposed to EtOH, with a focus on the involvement of Silent Mating Type Information Regulation 2 Homolog 1 (SIRT1)/Forkhead fox-O-1 (FOXO1) signaling pathway, and to explore the underlying molecular mechanisms.

METHODS: C57BL/6 adolescent male mice were given EtOH via intraperitoneal injection for 2 consecutive days, and the changes in the livers were detected via hematoxylin-eosin staining. The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured by biochemical methods. Protein expression of SIRT1, FOXO1, acetylated FOXO1 (ac-FOXO1), GRP78, p-eukaryotic initiation factor-2 (eIF2α), and apoptosis (p-JNK, p-c-Jun, and Bax) parameters was determined by Western blot. Reactive oxygen species (ROS) was detected by flow cytometry. Human hepatocytes Chang cell line was used to assay cell apoptosis by Annexin V and propidium iodide. In addition, mRNA levels of SIRT1, tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and monocyte chemoattractant protein 1 in liver tissues were detected by real-time polymerase chain reaction.

RESULTS: This study demonstrated that C3G (10 mg/kg) administration diminished EtOH-induced acute liver injury compared to control group, as evidenced by the significant decreases in ALT and AST levels. Pretreatment with C3G exerted anti-inflammatory effects as indicated by the decreased TNF-α and IL-6 levels, as well as decreased inflammatory foci and ballooning cells in liver tissue. The lessened hepatic injury was associated with enhanced SIRT1 protein expression and activity by C3G in vitro and in vivo. C3G treatment also provoked significant attenuation of endoplasmic reticulum stress parameters (GRP78, p-eIF2α), which was consistent with reduced levels of both p-c-Jun and Bax. Interestingly, EX527 inhibitor did not affect the protective function of C3G on alcohol-induced cell apoptosis. Moreover, alcohol exposure increased ROS level and decreased ac-FOXO1, while C3G intervention reversed this abnormality, and this may be related to SIRT1 activity by C3G.

CONCLUSIONS: Anthocyanin C3G has significant potency in antioxidant, anti-inflammatory, and anti-apoptotic effects on hepatocytes exposed to EtOH by modulating the SIRT1/FOXO1 signaling pathway. Our findings illustrate a novel and definitive therapeutic action of C3G and represent an economically feasible therapeutic intervention to treat alcoholic liver disease.

Copyright © 2016 by the Research Society on Alcoholism.

Keywords: Cyanidin 3-O-β-Glucoside; Endoplasmic Reticulum Stress; Forkhead Fox-O-1 (FOXO1); Reactive Oxygen Species; Silent Mating Type Information Regulation 2 Homolog 1 (SIRT1)

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