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Ismiyati A, Wiyasa IW, Hidayati DY. Protective Effect of Vitamins C and E on Depot-Medroxyprogesterone Acetate-Induced Ovarian Oxidative Stress In Vivo. J Toxicol. 2016;2016:3134105doi: 10.1155/2016/3134105.
Ismiyati, A., Wiyasa, I. W., & Hidayati, D. Y. (2016). Protective Effect of Vitamins C and E on Depot-Medroxyprogesterone Acetate-Induced Ovarian Oxidative Stress In Vivo. Journal of toxicology, 20163134105. https://doi.org/10.1155/2016/3134105
Ismiyati, Atik, et al. "Protective Effect of Vitamins C and E on Depot-Medroxyprogesterone Acetate-Induced Ovarian Oxidative Stress In Vivo." Journal of toxicology vol. 2016 (2016): 3134105. doi: https://doi.org/10.1155/2016/3134105
Ismiyati A, Wiyasa IW, Hidayati DY. Protective Effect of Vitamins C and E on Depot-Medroxyprogesterone Acetate-Induced Ovarian Oxidative Stress In Vivo. J Toxicol. 2016;2016:3134105. doi: 10.1155/2016/3134105. Epub 2016 Feb 07. PMID: 26966434; PMCID: PMC4761384.
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J Toxicol. 2016;2016:3134105. doi: 10.1155/2016/3134105. Epub 2016 Feb 07.

Protective Effect of Vitamins C and E on Depot-Medroxyprogesterone Acetate-Induced Ovarian Oxidative Stress In Vivo.

Journal of toxicology

Atik Ismiyati, I Wayan Arsana Wiyasa, Dwi Yuni Nur Hidayati

Affiliations

  1. Yogyakarta Midwifery Academy, Jalan Parangtritis Km 6, Sewon, Bantul, Yogyakarta Special Region 55188, Indonesia.
  2. Division of Fertility, Endocrinology and Reproduction, Obstetric and Ginaecology Laboratory, Saiful Anwar General Hospital, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia.
  3. Microbiology Laboratory, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia.

PMID: 26966434 PMCID: PMC4761384 DOI: 10.1155/2016/3134105

Abstract

A study was designed to investigate ameliorates effect of combined vitamins C and E able to against depot-medroxyprogesterone acetate- (DMPA-) induced ovarian oxidative stress in rat. Twenty-five female Wistar rats were divided into the following groups (n = 5 rats each): control (untreated) (C); depot-medroxyprogesterone acetate (DMPA); DMPA plus green vitamin C (at dose of 0.2 mg/gram; 0.4 mg/gram; 0.8 mg/gram) and vitamin E (0.04 IU/gram). The treatment with combined vitamins C and E was performed for four weeks. Analysis of malondialdehyde (MDA) level as a marker of oxidative stress was done colorimetrically. Analysis of SOD level was done by enzyme linked immunosorbent assay (ELISA) technically. This increase in ovarium MDA was significantly (P < 0.05) attenuated by medium dose treatments of combined vitamins C and E. DMPA insignificantly decreased SOD levels compared to the untreated group. This decrease in ovarian SOD level was significantly attenuated by all doses of the combined vitamins C and E. In conclusion, DMPA induces ovarian oxidative stress. Combined vitamins C and E prohibit the increase in ovarian lipid peroxidation, at least in part by modulating of superoxide dismutase. Therefore, this may provide an antioxidant therapy for attenuating the ovarian toxicity found in the DMPA therapy.

References

  1. Acta Physiol Hung. 2010 Jun;97(2):208-15 - PubMed
  2. Obstet Gynecol Clin North Am. 2000 Dec;27(4):741-80 - PubMed
  3. Reprod Toxicol. 2006 Nov;22(4):783-90 - PubMed
  4. Placenta. 2008 Aug;29(8):680-90 - PubMed
  5. J Soc Gynecol Investig. 1998 Sep-Oct;5(5):271-6 - PubMed
  6. Contraception. 2004 Jul;70(1):11-8 - PubMed
  7. Contraception. 1988 May;37(5):529-38 - PubMed
  8. J Am Coll Nutr. 2003 Jun;22(3):239-46 - PubMed
  9. Am J Obstet Gynecol. 1977 May 1;128(1):60-74 - PubMed
  10. Toxicology. 2007 Mar 7;231(2-3):215-23 - PubMed
  11. Acta Endocrinol (Copenh). 1982 Mar;99(3):339-43 - PubMed
  12. Br J Nutr. 2000 Sep;84(3):261-7 - PubMed
  13. Acta Obstet Gynecol Scand. 2005 Mar;84(3):207-16 - PubMed
  14. Hum Pathol. 2013 Oct;44(10):2293-301 - PubMed
  15. Free Radic Biol Med. 2011 Sep 1;51(5):1000-13 - PubMed
  16. Toxicol Pathol. 2005;33(3):409-12 - PubMed
  17. Free Radic Biol Med. 1993 Jun;14(6):649-53 - PubMed
  18. Contraception. 2009 Aug;80(2):187-93 - PubMed
  19. Environ Toxicol Pharmacol. 2007 Mar;23(2):221-7 - PubMed
  20. Clin Ther. 2004 Nov;26(11):1845-54 - PubMed
  21. Contraception. 2012 Jul;86(1):62-6 - PubMed

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