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Naqqash T, Hameed S, Imran A, et al. Differential Response of Potato Toward Inoculation with Taxonomically Diverse Plant Growth Promoting Rhizobacteria. Front Plant Sci. 2016;7:144doi: 10.3389/fpls.2016.00144.
Naqqash, T., Hameed, S., Imran, A., Hanif, M. K., Majeed, A., & van Elsas, J. D. (2016). Differential Response of Potato Toward Inoculation with Taxonomically Diverse Plant Growth Promoting Rhizobacteria. Frontiers in plant science, 7144. https://doi.org/10.3389/fpls.2016.00144
Naqqash, Tahir, et al. "Differential Response of Potato Toward Inoculation with Taxonomically Diverse Plant Growth Promoting Rhizobacteria." Frontiers in plant science vol. 7 (2016): 144. doi: https://doi.org/10.3389/fpls.2016.00144
Naqqash T, Hameed S, Imran A, Hanif MK, Majeed A, van Elsas JD. Differential Response of Potato Toward Inoculation with Taxonomically Diverse Plant Growth Promoting Rhizobacteria. Front Plant Sci. 2016 Feb 17;7:144. doi: 10.3389/fpls.2016.00144. eCollection 2016. PMID: 26925072; PMCID: PMC4756182.
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Front Plant Sci. 2016 Feb 17;7:144. doi: 10.3389/fpls.2016.00144. eCollection 2016.

Differential Response of Potato Toward Inoculation with Taxonomically Diverse Plant Growth Promoting Rhizobacteria.

Frontiers in plant science

Tahir Naqqash, Sohail Hameed, Asma Imran, Muhammad Kashif Hanif, Afshan Majeed, Jan Dirk van Elsas

Affiliations

  1. National Institute for Biotechnology and Genetic EngineeringFaisalabad, Pakistan; Pakistan Institute of Engineering and Applied SciencesIslamabad, Pakistan.
  2. National Institute for Biotechnology and Genetic Engineering Faisalabad, Pakistan.
  3. National Institute for Biotechnology and Genetic EngineeringFaisalabad, Pakistan; University of PoonchRawlakot, Pakistan.
  4. Department of Microbial Ecology, Groningen Institute for Evolutionary Life Sciences, University of Groningen Groningen, Netherlands.

PMID: 26925072 PMCID: PMC4756182 DOI: 10.3389/fpls.2016.00144

Abstract

Rhizosphere engineering with beneficial plant growth promoting bacteria offers great promise for sustainable crop yield. Potato is an important food commodity that needs large inputs of nitrogen and phosphorus fertilizers. To overcome high fertilizer demand (especially nitrogen), five bacteria, i.e., Azospirillum sp. TN10, Agrobacterium sp. TN14, Pseudomonas sp. TN36, Enterobacter sp. TN38 and Rhizobium sp. TN42 were isolated from the potato rhizosphere on nitrogen-free malate medium and identified based on their 16S rRNA gene sequences. Three strains, i.e., TN10, TN38, and TN42 showed nitrogen fixation (92.67-134.54 nmol h(-1)mg(-1) protein), while all showed the production of indole-3-acetic acid (IAA), which was significantly increased by the addition of L-tryptophan. Azospirillum sp. TN10 produced the highest amount of IAA, as measured by spectrophotometry (312.14 μg mL(-1)) and HPLC (18.3 μg mL(-1)). Inoculation with these bacteria under axenic conditions resulted in differential growth responses of potato. Azospirillum sp. TN10 incited the highest increase in potato fresh and dry weight over control plants, along with increased N contents of shoot and roots. All strains were able to colonize and maintain their population densities in the potato rhizosphere for up to 60 days, with Azospirillum sp. and Rhizobium sp. showing the highest survival. Plant root colonization potential was analyzed by transmission electron microscopy of root sections inoculated with Azospirillum sp. TN10. Of the five test strains, Azospirillum sp. TN10 has the greatest potential to increase the growth and nitrogen uptake of potato. Hence, it is suggested as a good candidate for the production of potato biofertilizer for integrated nutrient management.

Keywords: Azospirillum; IAA; N2-fixation; PGPR; TEM; plant inoculation; potato

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