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Larsen B, Ørgaard M, Toldam-Andersen TB, et al. A high-throughput method for genotyping S-RNase alleles in apple. Mol Breed. 2016;36:24doi: 10.1007/s11032-016-0448-0.
Larsen, B., Ørgaard, M., Toldam-Andersen, T. B., & Pedersen, C. (2016). A high-throughput method for genotyping S-RNase alleles in apple. Molecular breeding : new strategies in plant improvement, 3624. https://doi.org/10.1007/s11032-016-0448-0
Larsen, Bjarne, et al. "A high-throughput method for genotyping S-RNase alleles in apple." Molecular breeding : new strategies in plant improvement vol. 36 (2016): 24. doi: https://doi.org/10.1007/s11032-016-0448-0
Larsen B, Ørgaard M, Toldam-Andersen TB, Pedersen C. A high-throughput method for genotyping S-RNase alleles in apple. Mol Breed. 2016;36:24. doi: 10.1007/s11032-016-0448-0. Epub 2016 Feb 19. PMID: 26941563; PMCID: PMC4760992.
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Mol Breed. 2016;36:24. doi: 10.1007/s11032-016-0448-0. Epub 2016 Feb 19.

A high-throughput method for genotyping S-RNase alleles in apple.

Molecular breeding : new strategies in plant improvement

Bjarne Larsen, Marian Ørgaard, Torben Bo Toldam-Andersen, Carsten Pedersen

Affiliations

  1. Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 3rd Floor, 1871 Frederiksberg C, Denmark.

PMID: 26941563 PMCID: PMC4760992 DOI: 10.1007/s11032-016-0448-0

Abstract

We present a new efficient screening tool for detection of S-alleles in apple. The protocol using general and multiplexed primers for PCR reaction and fragment detection on an automatized capillary DNA sequencer exposed a higher number of alleles than any previous studies. Analysis of alleles is made on basis of three individual fragment sizes making the allele interpretation highly accurate. The method was employed to genotype 432

Keywords: Apple; Breeding; Compatibility; Fragment analysis; Malus domestica; S-RNase alleles

References

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  3. Nucleic Acids Res. 2007 Jul;35(Web Server issue):W71-4 - PubMed
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