Cell Div. 2016 Mar 18;11:3. doi: 10.1186/s13008-016-0015-4. eCollection 2016.
Mitochondria-cytoskeleton associations in mammalian cytokinesis.
Cell division
E J Lawrence, E Boucher, C A Mandato
Affiliations
Affiliations
- Department of Anatomy and Cell Biology, McGill University, Montreal, QC Canada.
PMID: 27030796
PMCID: PMC4812650 DOI: 10.1186/s13008-016-0015-4
Abstract
BACKGROUND: The role of the cytoskeleton in regulating mitochondrial distribution in dividing mammalian cells is poorly understood. We previously demonstrated that mitochondria are transported to the cleavage furrow during cytokinesis in a microtubule-dependent manner. However, the exact subset of spindle microtubules and molecular machinery involved remains unknown.
METHODS: We employed quantitative imaging techniques and structured illumination microscopy to analyse the spatial and temporal relationship of mitochondria with microtubules and actin of the contractile ring during cytokinesis in HeLa cells.
RESULTS: Superresolution microscopy revealed that mitochondria were associated with astral microtubules of the mitotic spindle in cytokinetic cells. Dominant-negative mutants of KIF5B, the heavy chain of kinesin-1 motor, and of Miro-1 disrupted mitochondrial transport to the furrow. Live imaging revealed that mitochondrial enrichment at the cell equator occurred simultaneously with the appearance of the contractile ring in cytokinesis. Inhibiting RhoA activity and contractile ring assembly with C3 transferase, caused mitochondrial mislocalisation during division.
CONCLUSIONS: Taken together, the data suggest a model in which mitochondria are transported by a microtubule-mediated mechanism involving equatorial astral microtubules, Miro-1, and KIF5B to the nascent actomyosin contractile ring in cytokinesis.
Keywords: Actin; Cytokinesis; Cytoskeleton; KIF5B; Microtubules; Miro; Mitochondria
References
- Commun Integr Biol. 2013 Nov 1;6(6):e27557 - PubMed
- J Neurosci. 2010 Mar 10;30(10):3555-66 - PubMed
- Nat Cell Biol. 2011 Jun 12;13(7):771-8 - PubMed
- Cell Motil Cytoskeleton. 2000 Feb;45(2):87-92 - PubMed
- Annu Rev Genet. 2009;43:95-118 - PubMed
- Biochem Biophys Res Commun. 2006 Jun 2;344(2):500-10 - PubMed
- Neuroscience. 1991;40(2):587-98 - PubMed
- Dev Cell. 2015 Aug 10;34(3):323-37 - PubMed
- Nature. 2015 Feb 5;518(7537):111-4 - PubMed
- J Cell Sci. 2011 Jul 15;124(Pt 14):2457-65 - PubMed
- J Cell Biol. 2004 Oct 11;167(1):87-98 - PubMed
- J Neurosci. 1998 Oct 15;18(20):8300-10 - PubMed
- Nat Cell Biol. 2001 Jan;3(1):8-14 - PubMed
- Genetics. 1996 Nov;144(3):1075-85 - PubMed
- Cold Spring Harb Perspect Biol. 2013 Jun 01;5(6):null - PubMed
- Cell. 1998 Jun 26;93(7):1147-58 - PubMed
- J Cell Biol. 2006 May 22;173(4):545-57 - PubMed
- Nature. 2005 Oct 13;437(7061):1043-7 - PubMed
- J Cell Sci. 1993 Mar;104 ( Pt 3):917-27 - PubMed
- J Mol Cell Cardiol. 2014 Aug;73:26-33 - PubMed
- J Biol Chem. 2003 Feb 21;278(8):6495-502 - PubMed
- Biophys J. 2010 Oct 6;99(7):2143-52 - PubMed
- J Cell Biol. 2015 Jan 5;208(1):109-23 - PubMed
- J Cell Biol. 2003 Jan 20;160(2):255-65 - PubMed
- Trends Cell Biol. 2005 Dec;15(12):651-8 - PubMed
- Mol Biol Cell. 2006 Apr;17 (4):2057-68 - PubMed
- J Biol Chem. 1993 Nov 5;268(31):23215-8 - PubMed
- J Cell Biol. 2014 Mar 31;204(7):1087-98 - PubMed
- Cell Cycle. 2012 Aug 1;11(15):2864-75 - PubMed
- J Cell Sci. 2005 Apr 15;118(Pt 8):1549-58 - PubMed
- J Cell Sci. 2006 Feb 15;119(Pt 4):659-70 - PubMed
- Neuron. 2013 Feb 6;77(3):485-502 - PubMed
- EMBO J. 1997 Jun 2;16(11):3044-56 - PubMed
- J Cell Sci. 2014 Feb 1;127(Pt 3):477-83 - PubMed
- Curr Biol. 2006 Jul 25;16(14 ):R551-60 - PubMed
- J Cell Biol. 1999 Jan 25;144(2):305-13 - PubMed
- PLoS One. 2013 Aug 21;8(8):e72886 - PubMed
- Oncogene. 2000 Dec 7;19(52):6059-64 - PubMed
- Endocrinology. 2010 Sep;151(9):4313-23 - PubMed
- Neuron. 2002 Dec 19;36(6):1063-77 - PubMed
- Cell. 1994 Dec 30;79(7):1209-20 - PubMed
- Cell Calcium. 2012 Jul;52(1):57-63 - PubMed
- Nat Commun. 2015 Aug 11;6:8015 - PubMed
- Annu Rev Biochem. 2006;75:543-66 - PubMed
- J Cell Sci. 2012 May 1;125(Pt 9):2095-104 - PubMed
- J Neurosci. 2000 Sep 1;20(17 ):6374-84 - PubMed
- J Neurocytol. 1993 Nov;22(11):925-40 - PubMed
- Curr Biol. 2014 Nov 3;24(21):2598-605 - PubMed
- Nat Rev Mol Cell Biol. 2012 Sep;13(9):566-78 - PubMed
- Brain Res. 2004 Nov 12;1026(2):235-43 - PubMed
- Mol Biol Cell. 2003 May;14(5):1745-56 - PubMed
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