J Ther Ultrasound. 2016 Mar 29;4:10. doi: 10.1186/s40349-016-0053-z. eCollection 2016.
In-vitro cell treatment with focused shockwaves-influence of the experimental setup on the sound field and biological reaction.
Journal of therapeutic ultrasound
Kristin Dietz-Laursonn, Rainer Beckmann, Siegfried Ginter, Klaus Radermacher, Matías de la Fuente
Affiliations
Affiliations
- Chair of medical engineering, RWTH Aachen University, Pauwelsstraße 20, Aachen, 52074 Germany.
- Department of Anatomy and Cell Biology, RWTH Aachen University, Wendlingweg 2, Aachen, 52074 Germany.
- Richard Wolf GmbH, Pforzheimer Straße 32, Knittlingen, 75438 Germany.
PMID: 27030807
PMCID: PMC4812651 DOI: 10.1186/s40349-016-0053-z
Abstract
BACKGROUND: To improve understanding of shockwave therapy mechanisms, in vitro experiments are conducted and the correlation between cell reaction and shockwave parameters like the maximum pressure or energy density is studied. If the shockwave is not measured in the experimental setup used, it is usually assumed that the device's shockwave parameters (=manufacturer's free field measurements) are valid. But this applies only for in vitro setups which do not modify the shockwave, e.g., by reflection or refraction. We hypothesize that most setups used for in vitro shockwave experiments described in the literature influence the sound field significantly so that correlations between the physical parameters and the biological reaction are not valid.
METHODS: To reveal the components of common shockwave in vitro setups which mainly influence the sound field, 32 publications with 37 setups used for focused shockwave experiments were reviewed and evaluated regarding cavitation, cell container material, focal sound field size relative to cell model size, and distance between treated cells and air. For further evaluation of the severity of those influences, experiments and calculations were conducted.
RESULTS: In 37 setups, 17 different combinations of coupling, cell container, and cell model are described. The setup used mainly is a transducer coupled via water to a tube filled with a cell suspension. As changes of the shockwaves' maximum pressure of 11 % can already induce changes of the biological reaction, the sound field and biological reactions are mainly disturbed by use of standard cell containers, use of coupling gel, air within the 5 MPa focal zone, and cell model sizes which are bigger than half the -6 dB focal dimensions.
CONCLUSIONS: Until now, correct and sufficient information about the shockwave influencing cells in vitro is only provided in 1 of 32 publications. Based on these findings, guidelines for improved in vitro setups are proposed which help minimize the influence of the setup on the sound field.
Keywords: ESWT; Experimental setup; In vitro; Review; Shockwave; Shockwave parameters; Therapy
References
- Cancer Res. 1999 Oct 15;59(20):5227-32 - PubMed
- J Urol. 2006 Dec;176(6 Pt 1):2706-10 - PubMed
- Ultrasound Med Biol. 1990;16(6):587-94 - PubMed
- Urol Res. 1988;16(6):419-26 - PubMed
- J Urol. 2002 Oct;168(4 Pt 1):1556-62 - PubMed
- J Foot Ankle Res. 2015 Feb 05;8:3 - PubMed
- Phys Med Biol. 2001 Feb;46(2):413-37 - PubMed
- Stem Cell Res. 2013 Sep;11(2):951-64 - PubMed
- Urology. 1991 Dec;38(6):571-6 - PubMed
- Urol Res. 2010 Aug;38(4):321-6 - PubMed
- Ultrasound Med Biol. 2005 Jan;31(1):115-9 - PubMed
- Biomed Tech (Berl). 2002;47 Suppl 1 Pt 1:382-5 - PubMed
- J Vis Exp. 2014 Apr 08;(86):null - PubMed
- Biochem Biophys Res Commun. 2001 Sep 28;287(3):648-55 - PubMed
- Coron Artery Dis. 2006 Feb;17(1):63-70 - PubMed
- Cancer Sci. 2006 Apr;97(4):296-304 - PubMed
- Phys Med Biol. 2001 Apr;46(4):1245-64 - PubMed
- J Bone Joint Surg Am. 2002 Mar;84-A(3):335-41 - PubMed
- Vasc Med. 2011 Oct;16(5):365-77 - PubMed
- Arch Orthop Trauma Surg. 2003 Sep;123(7):345-8 - PubMed
- J Surg Res. 1994 Dec;57(6):677-81 - PubMed
- Ultrasound Med Biol. 2001 May;27(5):665-71 - PubMed
- Ultrasound Med Biol. 1996;22(9):1131-54 - PubMed
- J Trauma. 2008 Dec;65(6):1402-10 - PubMed
- Ultrasound Med Biol. 1989;15(3):213-27 - PubMed
- Urol Res. 1990;18(4):255-8 - PubMed
- J Urol. 1991 Jan;145(1):171-5 - PubMed
- Breast Cancer Res Treat. 2003 Sep;81(1):11-9 - PubMed
- Acta Biochim Biophys Sin (Shanghai). 2004 Nov;36(11):741-8 - PubMed
- Clin Orthop Relat Res. 2001 Jun;(387):95-101 - PubMed
- Ultrasonics. 2014 Jan;54(1):131-6 - PubMed
- Ultrasound Med Biol. 2008 May;34(5):841-52 - PubMed
- J Acoust Soc Am. 2008 Oct;124(4):2406-20 - PubMed
- Ultrasound Med Biol. 1999 Mar;25(3):473-9 - PubMed
- J Surg Res. 2014 Jan;186(1):484-92 - PubMed
- Int Orthop. 1991;15(3):181-4 - PubMed
- Z Orthop Ihre Grenzgeb. 2004 Jul-Aug;142(4):462-6 - PubMed
- Ultrasound Med Biol. 1997;23 (6):939-52 - PubMed
- Biomed Tech (Berl). 1998 Oct;43(10):269-74 - PubMed
- Ultrasound Med Biol. 1989;15(5):451-60 - PubMed
- J Acoust Soc Am. 2011 Oct;130(4):1888-98 - PubMed
- Ultrasound Med Biol. 2007 Sep;33(9):1495-503 - PubMed
- IEEE Trans Ultrason Ferroelectr Freq Control. 1998;45(3):788-99 - PubMed
- Artif Cells Blood Substit Immobil Biotechnol. 2003 Nov;31(4):449-66 - PubMed
- Oncol Rep. 2006 Jan;15(1):267-73 - PubMed
- Diabetes Res Clin Pract. 2011 May;92(2):187-93 - PubMed
- Burns. 2010 Sep;36(6):844-9 - PubMed
- Ultrasound Med Biol. 1998 Sep;24(7):1055-9 - PubMed
- Arch Orthop Trauma Surg. 2009 May;129(5):641-7 - PubMed
- J Acoust Soc Am. 1998 Nov;104(5):3126-9 - PubMed
- Ultrasound Med Biol. 2011 Dec;37(12):2105-15 - PubMed
- Urol Res. 1989;17(1):13-9 - PubMed
- Ostomy Wound Manage. 2014 Jul;60(7):26-39 - PubMed
- Br J Rheumatol. 1994 Oct;33(10):901-8 - PubMed
- Eur J Cancer Clin Oncol. 1989 Aug;25(8):1173-9 - PubMed
- Ann Rheum Dis. 2003 Mar;62(3):248-50 - PubMed
- Z Orthop Ihre Grenzgeb. 2000 Jan-Feb;138(1):29-33 - PubMed
- Acta Orthop. 2009 Oct;80(5):612-7 - PubMed
- Ultrasound Med Biol. 2008 Feb;34(2):334-42 - PubMed
- Ultrasound Med Biol. 1992;18(8):691-9 - PubMed
- Arch Orthop Trauma Surg. 2011 Mar;131(3):303-9 - PubMed
- Circulation. 2000 Jul 11;102(2):238-45 - PubMed
- J Urol. 1989 Apr;141(4):965-8 - PubMed
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