Display options
Cite
Pande VV, Devon RL, Sharma P, et al. Study of Salmonella Typhimurium Infection in Laying Hens. Front Microbiol. 2016;7:203doi: 10.3389/fmicb.2016.00203.
Pande, V. V., Devon, R. L., Sharma, P., McWhorter, A. R., & Chousalkar, K. K. (2016). Study of Salmonella Typhimurium Infection in Laying Hens. Frontiers in microbiology, 7203. https://doi.org/10.3389/fmicb.2016.00203
Pande, Vivek V, et al. "Study of Salmonella Typhimurium Infection in Laying Hens." Frontiers in microbiology vol. 7 (2016): 203. doi: https://doi.org/10.3389/fmicb.2016.00203
Pande VV, Devon RL, Sharma P, McWhorter AR, Chousalkar KK. Study of Salmonella Typhimurium Infection in Laying Hens. Front Microbiol. 2016 Feb 25;7:203. doi: 10.3389/fmicb.2016.00203. eCollection 2016. PMID: 26941727; PMCID: PMC4766288.
Copy Download .nbib Format: NLM
Share it on

Front Microbiol. 2016 Feb 25;7:203. doi: 10.3389/fmicb.2016.00203. eCollection 2016.

Study of Salmonella Typhimurium Infection in Laying Hens.

Frontiers in microbiology

Vivek V Pande, Rebecca L Devon, Pardeep Sharma, Andrea R McWhorter, Kapil K Chousalkar

Affiliations

  1. School of Animal and Veterinary Sciences, The University of Adelaide Roseworthy, SA, Australia.

PMID: 26941727 PMCID: PMC4766288 DOI: 10.3389/fmicb.2016.00203

Abstract

Members of Salmonella enterica are frequently involved in egg and egg product related human food poisoning outbreaks worldwide. In Australia, Salmonella Typhimurium is frequently involved in egg and egg product related foodborne illness and Salmonella Mbandaka has also been found to be a contaminant of the layer farm environment. The ability possessed by Salmonella Enteritidis to colonize reproductive organs and contaminate developing eggs has been well-described. However, there are few studies investigating this ability for Salmonella Typhimurium. The hypothesis of this study was that the Salmonella Typhimurium can colonize the gut for a prolonged period of time and that horizontal infection through feces is the main route of egg contamination. At 14 weeks of age hens were orally infected with either S. Typhimurium PT 9 or S. Typhimurium PT 9 and Salmonella Mbandaka. Salmonella shedding in feces and eggs was monitored for 15 weeks post-infection. Egg shell surface and internal contents of eggs laid by infected hens were cultured independently for detection of Salmonella spp. The mean Salmonella load in feces ranged from 1.54 to 63.35 and 0.31 to 98.38 most probable number/g (MPN/g) in the S. Typhimurium and S. Typhimurium + S. Mbandaka group, respectively. No correlation was found between mean fecal Salmonella load and frequency of egg shell contamination. Egg shell contamination was higher in S. Typhimurium + S. Mbandaka infected group (7.2% S. Typhimurium, 14.1% S. Mbandaka) compared to birds infected with S. Typhimurium (5.66%) however, co-infection had no significant impact on egg contamination by S. Typhimurium. Throughout the study Salmonella was not recovered from internal contents of eggs laid by hens. Salmonella was isolated from different segments of oviduct of hens from both the groups, however pathology was not observed on microscopic examination. This study investigated Salmonella shedding for up to 15 weeks p.i which is a longer period of time compared to previously published studies. The findings of current study demonstrated intermittent but persistent fecal shedding of Salmonella after oral infection for up to 15 weeks p.i. Further, egg shell contamination, with lack of internal egg content contamination and the low frequency of reproductive organ infection suggested that horizontal infection through contaminated feces is the main route of egg contamination with S. Typhimurium in laying hens.

Keywords: Salmonella Typhimurium; egg contamination; laying hens; oviduct

References

  1. FEMS Microbiol Rev. 2009 Jul;33(4):718-38 - PubMed
  2. Vet Rec. 1998 Nov 14;143(20):562-3 - PubMed
  3. Int J Environ Res Public Health. 2015 Feb 26;12(3):2543-56 - PubMed
  4. Avian Dis. 1997 Jul-Sep;41(3):535-9 - PubMed
  5. Commun Dis Intell Q Rep. 2009 Dec;33(4):389-413 - PubMed
  6. Poult Sci. 1994 Jan;73(1):89-98 - PubMed
  7. Int J Food Microbiol. 2015 Jun 16;203:23-6 - PubMed
  8. Br Poult Sci. 2006 Aug;47(4):379-91 - PubMed
  9. PLoS One. 2014 Mar 12;9(3):e90987 - PubMed
  10. BMC Microbiol. 2009 Dec 19;9:268 - PubMed
  11. FEMS Microbiol Lett. 1999 Feb 15;171(2):203-7 - PubMed
  12. Poult Sci. 2015 Jul;94(7):1691-8 - PubMed
  13. Appl Environ Microbiol. 2015 Dec;81(23):8192-201 - PubMed
  14. Avian Dis. 2001 Jan-Mar;45(1):61-9 - PubMed
  15. J Clin Microbiol. 2014 Sep;52(9):3250-8 - PubMed
  16. Avian Dis. 2001 Oct-Dec;45(4):962-71 - PubMed
  17. Poult Sci. 2010 Aug;89(8):1629-34 - PubMed
  18. J Appl Microbiol. 2010 Jul;109(1):25-34 - PubMed
  19. Infect Immun. 1995 Jul;63(7):2443-9 - PubMed
  20. Appl Environ Microbiol. 1997 Oct;63(10):3741-51 - PubMed
  21. J Microbiol Methods. 2011 Apr;85(1):9-15 - PubMed
  22. Commun Dis Intell Q Rep. 2012 Sep 30;36(3):E213-41 - PubMed
  23. J Food Prot. 2007 Oct;70(10):2259-65 - PubMed
  24. Avian Dis. 2004 Dec;48(4):863-9 - PubMed
  25. Int J Food Microbiol. 2006 Dec 1;112(3):253-60 - PubMed
  26. BMC Microbiol. 2009 Jan 06;9:3 - PubMed
  27. Appl Environ Microbiol. 2015 Jan;81(1):405-14 - PubMed
  28. Appl Environ Microbiol. 2011 Jul;77(13):4273-9 - PubMed
  29. Avian Pathol. 2011 Oct;40(5):429-36 - PubMed
  30. Int J Food Microbiol. 2014 Oct 17;189:61-6 - PubMed
  31. Avian Pathol. 2008 Aug;37(4):399-406 - PubMed
  32. Vet Immunol Immunopathol. 2003 Oct 15;95(3-4):91-101 - PubMed
  33. Avian Pathol. 1988;17(3):571-88 - PubMed
  34. BMC Microbiol. 2013 Jun 07;13:128 - PubMed
  35. Poult Sci. 1973 Mar;52(2):661-6 - PubMed
  36. Int J Food Microbiol. 2013 Jun 17;164(2-3):161-5 - PubMed
  37. Vet Microbiol. 2001 May 21;80(2):139-48 - PubMed
  38. Microbiol Mol Biol Rev. 2013 Dec;77(4):582-607 - PubMed
  39. Rev Sci Tech. 1995 Jun;14(2):365-80 - PubMed
  40. J Food Prot. 2013 Jan;76(1):108-13 - PubMed
  41. Avian Dis. 2007 Mar;51(1):40-4 - PubMed
  42. Poult Sci. 1980 May;59(5):1067-72 - PubMed

Publication Types