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Heikinheimo A, Johler S, Karvonen L, et al. New dominant spa type t2741 in livestock-associated MRSA (CC398-MRSA-V) in Finnish fattening pigs at slaughter. Antimicrob Resist Infect Control. 2016;5:6doi: 10.1186/s13756-016-0105-8.
Heikinheimo, A., Johler, S., Karvonen, L., Julmi, J., Fredriksson-Ahomaa, M., & Stephan, R. (2016). New dominant spa type t2741 in livestock-associated MRSA (CC398-MRSA-V) in Finnish fattening pigs at slaughter. Antimicrobial resistance and infection control, 56. https://doi.org/10.1186/s13756-016-0105-8
Heikinheimo, Annamari, et al. "New dominant spa type t2741 in livestock-associated MRSA (CC398-MRSA-V) in Finnish fattening pigs at slaughter." Antimicrobial resistance and infection control vol. 5 (2016): 6. doi: https://doi.org/10.1186/s13756-016-0105-8
Heikinheimo A, Johler S, Karvonen L, Julmi J, Fredriksson-Ahomaa M, Stephan R. New dominant spa type t2741 in livestock-associated MRSA (CC398-MRSA-V) in Finnish fattening pigs at slaughter. Antimicrob Resist Infect Control. 2016 Mar 02;5:6. doi: 10.1186/s13756-016-0105-8. eCollection 2016. PMID: 26941953; PMCID: PMC4776440.
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Antimicrob Resist Infect Control. 2016 Mar 02;5:6. doi: 10.1186/s13756-016-0105-8. eCollection 2016.

New dominant spa type t2741 in livestock-associated MRSA (CC398-MRSA-V) in Finnish fattening pigs at slaughter.

Antimicrobial resistance and infection control

Annamari Heikinheimo, Sophia Johler, Laura Karvonen, Jérôme Julmi, Maria Fredriksson-Ahomaa, Roger Stephan

Affiliations

  1. Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Agnes Sjöberginkatu 2, P.O. Box 66, FI 00014 Helsinki, Finland.
  2. Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Winterthurerstrasse 272, 8057 Zurich, Switzerland.

PMID: 26941953 PMCID: PMC4776440 DOI: 10.1186/s13756-016-0105-8

Abstract

BACKGROUND: The emergence of livestock-associated MRSA has become a growing public health concern worldwide. Studies elucidating the population structure, as well as resistance phenotypes and virulence gene profiles of livestock-associated MRSA strains are needed to improve risk assessment and to develop effective control measures. The objective of this study therefore was to determine i) clonal complexes and spa types, as well as ii) resistance phenotypes and iii) virulence and resistance gene profiles of livestock-associated MRSA isolated from Finnish fattening pigs at slaughter.

METHODS: Fifty MRSA isolates collected from Finnish fattening pigs at slaughter were characterized by spa typing and DNA microarray profiling. In addition, antimicrobial susceptibility testing was performed using the Kirby Bauer disk diffusion method.

RESULTS: MRSA isolates were assigned to clonal complexes CC1 (n = 4) and CC398 (n = 46). One dominant spa type (t2741) was present in 33 out of 50 investigated isolates, originating from 15 out of 18 farms. The remaining isolates were assigned to spa types t034 (n = 7), t108 (n = 5), and t011 (n = 1). Although each herd exhibited isolates assigned to one clonal complex only, five herds harbored MRSA isolates of either two or three different spa types. All tested MRSA isolates were phenotypically resistant to penicillin, oxacillin, cefoxitin, and tetracycline. With the exception of the isolates assigned to t108, all isolates exhibited resistance to clindamycin. On the genomic level, all isolates exhibited mecA, blaZ/I/R, and tetK, and were assigned to SCCmec type V. Many isolates also harbored tetM (46/50 isolates), lnuB (41/50 isolates), ermB (26/50 isolates), and one isolate was positive for aadD. DNA microarray profiling showed that all isolates of the dominant CC398/t2741 MRSA-V type belonged to agr type I, capsule type 5, and were negative for fnbB. Interestingly, one isolate of CC398/t2741 MRSA-V was agr negative and also lacked hld.

CONCLUSIONS: A new dominant LA-MRSA clone (CC398/t2741, SCCmec type V) was identified among fattening pigs in Finland. This is the first study identifying t2741 as a common spa type in LA-MRSA in pigs.

Keywords: Livestock-associated MRSA; Pig; Spa type 2741; agr; fnbB

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