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Wong TY, Chen YH, Liu SH, et al. Differential Proteomic Analysis of Human Placenta-Derived Mesenchymal Stem Cells Cultured on Normal Tissue Culture Surface and Hyaluronan-Coated Surface. Stem Cells Int. 2015;2016:2809192doi: 10.1155/2016/2809192.
Wong, T. Y., Chen, Y. H., Liu, S. H., Solis, M. A., Yu, C. H., Chang, C. H., & Huang, L. L. (2016). Differential Proteomic Analysis of Human Placenta-Derived Mesenchymal Stem Cells Cultured on Normal Tissue Culture Surface and Hyaluronan-Coated Surface. Stem cells international, 20162809192. https://doi.org/10.1155/2016/2809192
Wong, Tzyy Yue, et al. "Differential Proteomic Analysis of Human Placenta-Derived Mesenchymal Stem Cells Cultured on Normal Tissue Culture Surface and Hyaluronan-Coated Surface." Stem cells international vol. 2016 (2016): 2809192. doi: https://doi.org/10.1155/2016/2809192
Wong TY, Chen YH, Liu SH, Solis MA, Yu CH, Chang CH, Huang LL. Differential Proteomic Analysis of Human Placenta-Derived Mesenchymal Stem Cells Cultured on Normal Tissue Culture Surface and Hyaluronan-Coated Surface. Stem Cells Int. 2016;2016:2809192. doi: 10.1155/2016/2809192. Epub 2015 Dec 29. PMID: 27057169; PMCID: PMC4709773.
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Stem Cells Int. 2016;2016:2809192. doi: 10.1155/2016/2809192. Epub 2015 Dec 29.

Differential Proteomic Analysis of Human Placenta-Derived Mesenchymal Stem Cells Cultured on Normal Tissue Culture Surface and Hyaluronan-Coated Surface.

Stem cells international

Tzyy Yue Wong, Ying-Hui Chen, Szu-Heng Liu, Mairim Alexandra Solis, Chen-Hsiang Yu, Chiung-Hsin Chang, Lynn L H Huang

Affiliations

  1. Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
  2. Department of Obstetrics and Gynecology, National Cheng Kung University, Tainan 701, Taiwan.
  3. Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan; Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan 701, Taiwan; Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan; Research Center of Excellence in Regenerative Medicine, National Cheng Kung University, Tainan 701, Taiwan; Advanced Optoelectronic Technology Center, National Cheng Kung University, Tainan 701, Taiwan.

PMID: 27057169 PMCID: PMC4709773 DOI: 10.1155/2016/2809192

Abstract

Our previous results showed that hyaluronan (HA) preserved human placenta-derived mesenchymal stem cells (PDMSC) in a slow cell cycling mode similar to quiescence, the pristine state of stem cells in vivo, and HA was found to prevent murine adipose-derived mesenchymal stem cells from senescence. Here, stable isotope labeling by amino acid in cell culture (SILAC) proteomic profiling was used to evaluate the effects of HA on aging phenomenon in stem cells, comparing (1) old and young passage PDMSC cultured on normal tissue culture surface (TCS); (2) old passage on HA-coated surface (CHA) compared to TCS; (3) old and young passage on CHA. The results indicated that senescence-associated protein transgelin (TAGLN) was upregulated in old TCS. Protein CYR61, reportedly senescence-related, was downregulated in old CHA compared to old TCS. The SIRT1-interacting Nicotinamide phosphoribosyltransferase (NAMPT) increased by 2.23-fold in old CHA compared to old TCS, and is 0.48-fold lower in old TCS compared to young TCS. Results also indicated that components of endoplasmic reticulum associated degradation (ERAD) pathway were upregulated in old CHA compared to old TCS cells, potentially for overcoming stress to maintain cell function and suppress senescence. Our data points to pathways that may be targeted by HA to maintain stem cells youth.

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