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Dai GH, Liu N, Zhu JW, et al. Qi-Shen-Yi-Qi Dripping Pills Promote Angiogenesis of Ischemic Cardiac Microvascular Endothelial Cells by Regulating MicroRNA-223-3p Expression. Evid Based Complement Alternat Med. 2016;2016:5057328doi: 10.1155/2016/5057328.
Dai, G. H., Liu, N., Zhu, J. W., Yao, J., Yang, C., Ma, P. Z., & Song, X. B. (2016). Qi-Shen-Yi-Qi Dripping Pills Promote Angiogenesis of Ischemic Cardiac Microvascular Endothelial Cells by Regulating MicroRNA-223-3p Expression. Evidence-based complementary and alternative medicine : eCAM, 20165057328. https://doi.org/10.1155/2016/5057328
Dai, Guo-Hua, et al. "Qi-Shen-Yi-Qi Dripping Pills Promote Angiogenesis of Ischemic Cardiac Microvascular Endothelial Cells by Regulating MicroRNA-223-3p Expression." Evidence-based complementary and alternative medicine : eCAM vol. 2016 (2016): 5057328. doi: https://doi.org/10.1155/2016/5057328
Dai GH, Liu N, Zhu JW, Yao J, Yang C, Ma PZ, Song XB. Qi-Shen-Yi-Qi Dripping Pills Promote Angiogenesis of Ischemic Cardiac Microvascular Endothelial Cells by Regulating MicroRNA-223-3p Expression. Evid Based Complement Alternat Med. 2016;2016:5057328. doi: 10.1155/2016/5057328. Epub 2016 Feb 15. PMID: 27057198; PMCID: PMC4761670.
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Evid Based Complement Alternat Med. 2016;2016:5057328. doi: 10.1155/2016/5057328. Epub 2016 Feb 15.

Qi-Shen-Yi-Qi Dripping Pills Promote Angiogenesis of Ischemic Cardiac Microvascular Endothelial Cells by Regulating MicroRNA-223-3p Expression.

Evidence-based complementary and alternative medicine : eCAM

Guo-Hua Dai, Ning Liu, Jing-Wei Zhu, Jing Yao, Chun Yang, Pei-Ze Ma, Xian-Bo Song

Affiliations

  1. Department of Cardiology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China.
  2. Department of Cardiology, Jining Hospital of Traditional Chinese Medicine, Jining 272000, China.
  3. Shandong University of Traditional Chinese Medicine, Jinan 250014, China.
  4. Department of Cardiology and Brain, Weihai Hospital of Traditional Chinese Medicine, Weihai 264200, China.

PMID: 27057198 PMCID: PMC4761670 DOI: 10.1155/2016/5057328

Abstract

Traditional Chinese medicine (TCM) research shows that Qi-Shen-Yi-Qi Dripping Pills (QSYQ) can promote ischemic cardiac angiogenesis. Studies have shown that microRNAs (miRNAs) are the key component of gene regulation networks, which play a vital role in angiogenesis and cardiovascular disease. Mechanisms involving miRNA by which TCM promotes ischemic cardiac angiogenesis have not been reported. We found that microRNA-223-3p (mir-223-3p) was the core miRNA of angiogenesis of rats ischemic cardiac microvascular endothelial cells (CMECs) and inhibited angiogenesis by affecting RPS6KB1/HIF-1α signal pathway in previous study. Based on the results, we observed biological characteristics and optimal dosage for QSYQ intervening in rats ischemic CMECs angiogenesis and concluded that QSYQ low-dose group had the strongest ability to promote angiogenesis of ischemic myocardium. Using miRNA chip and real-time PCR techniques in this study, we identified mir-223-3p as the pivotal miRNA in QSYQ that regulated angiogenesis of ischemic CMECs. From real-time PCR and western blot analysis, research showed that gene and protein expression of factors located RPS6KB1/HIF-1α signaling pathway, including HIF-1α, VEGF, MAPK, PI3K, and AKT, were significantly upregulated by QSYQ to regulate angiogenesis of ischemic CMECs. This study showed that QSYQ promote ischemic cardiac angiogenesis by downregulating mir-223-3p expression in rats ischemic CMECs.

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