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Goo IB, Im JH, Gil HW, et al. Comparison of Cell and Nuclear Size Difference between Diploid and Induced Triploid in Marine Medaka, Oryzias dancena. Dev Reprod. 2015;19(3):127-34doi: 10.12717/DR.2015.19.3.127.
Goo, I. B., Im, J. H., Gil, H. W., Lim, S. G., & Park, I. S. (2015). Comparison of Cell and Nuclear Size Difference between Diploid and Induced Triploid in Marine Medaka, Oryzias dancena. Development & reproduction, 19(3), 127-34. https://doi.org/10.12717/DR.2015.19.3.127
Goo, In Bon, et al. "Comparison of Cell and Nuclear Size Difference between Diploid and Induced Triploid in Marine Medaka, Oryzias dancena." Development & reproduction vol. 19,3 (2015): 127-34. doi: https://doi.org/10.12717/DR.2015.19.3.127
Goo IB, Im JH, Gil HW, Lim SG, Park IS. Comparison of Cell and Nuclear Size Difference between Diploid and Induced Triploid in Marine Medaka, Oryzias dancena. Dev Reprod. 2015 Sep;19(3):127-34. doi: 10.12717/DR.2015.19.3.127. PMID: 27004269; PMCID: PMC4801045.
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Dev Reprod. 2015 Sep;19(3):127-34. doi: 10.12717/DR.2015.19.3.127.

Comparison of Cell and Nuclear Size Difference between Diploid and Induced Triploid in Marine Medaka, Oryzias dancena.

Development & reproduction

In Bon Goo, Jae Hyun Im, Hyun Woo Gil, Sang Gu Lim, In-Seok Park

Affiliations

  1. Inland Aquaculture Research Center, National Fisheries Research & Development Institute, Jinhae 645-758, Korea.
  2. Dept. of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University, Busan 606-791, Korea.
  3. Future Aquaculture Research Center, National Fisheries Research & Development Institute, Jeju 690-192, Korea.

PMID: 27004269 PMCID: PMC4801045 DOI: 10.12717/DR.2015.19.3.127

Abstract

The influence of triploidization on cell and nucleus size characteristics of the same tissues of erythrocyte, retina, kidney, hepatocyte and midgut epithelium in marine medaka, Oryzias dancena has been determined histologically. Induced triploid fish are produced by cold shock treatments. Likewise, the size of horizontal cell nucleus in inner nuclear layer of retina, ganglion cell nucleus in ganglion cell layer of retina, proximal tubule cell of kidney, hepatocytes and nuclear height of midgut epithelium all appear to be significantly larger than diploid (p<0.05). On the other hand, retina thickness is larger in diploid than induced triploid (p<0.05). Induced triploid shows low density of cell number. Results of this study suggest that same characteristics in the induced triploid exhibiting larger cells and nucleus sizes with fewer number of cells than the diploid can be useful criteria for the distinction between diploid and induced triploid, and also the ploidy level in marine medaka.

Keywords: Diploid; Histological observation; Induced triploid; Marine medaka

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