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Salgado LT, Cinelli LP, Viana NB, et al. A VANADIUM BROMOPEROXIDASE CATALYZES THE FORMATION OF HIGH-MOLECULAR-WEIGHT COMPLEXES BETWEEN BROWN ALGAL PHENOLIC SUBSTANCES AND ALGINATES(1). J Phycol. 2009;45(1):193-202doi: 10.1111/j.1529-8817.2008.00642.x.
Salgado, L. T., Cinelli, L. P., Viana, N. B., Tomazetto de Carvalho, R., De Souza Mourão, P. A., Teixeira, V. L., Farina, M., & Filho, A. G. (2009). A VANADIUM BROMOPEROXIDASE CATALYZES THE FORMATION OF HIGH-MOLECULAR-WEIGHT COMPLEXES BETWEEN BROWN ALGAL PHENOLIC SUBSTANCES AND ALGINATES(1). Journal of phycology, 45(1), 193-202. https://doi.org/10.1111/j.1529-8817.2008.00642.x
Salgado, Leonardo Tavares, et al. "A VANADIUM BROMOPEROXIDASE CATALYZES THE FORMATION OF HIGH-MOLECULAR-WEIGHT COMPLEXES BETWEEN BROWN ALGAL PHENOLIC SUBSTANCES AND ALGINATES(1)." Journal of phycology vol. 45,1 (2009): 193-202. doi: https://doi.org/10.1111/j.1529-8817.2008.00642.x
Salgado LT, Cinelli LP, Viana NB, Tomazetto de Carvalho R, De Souza Mourão PA, Teixeira VL, Farina M, Filho AG. A VANADIUM BROMOPEROXIDASE CATALYZES THE FORMATION OF HIGH-MOLECULAR-WEIGHT COMPLEXES BETWEEN BROWN ALGAL PHENOLIC SUBSTANCES AND ALGINATES(1). J Phycol. 2009 Feb;45(1):193-202. doi: 10.1111/j.1529-8817.2008.00642.x. PMID: 27033657.
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J Phycol. 2009 Feb;45(1):193-202. doi: 10.1111/j.1529-8817.2008.00642.x.

A VANADIUM BROMOPEROXIDASE CATALYZES THE FORMATION OF HIGH-MOLECULAR-WEIGHT COMPLEXES BETWEEN BROWN ALGAL PHENOLIC SUBSTANCES AND ALGINATES(1).

Journal of phycology

Leonardo Tavares Salgado, Leonardo Paes Cinelli, Nathan Bessa Viana, Rodrigo Tomazetto de Carvalho, Paulo Antônio De Souza Mourão, Valéria Laneuville Teixeira, Marcos Farina, And Gilberto Menezes Amado Filho

Affiliations

  1. Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, Diretoria de Pesquisas, MMA, 22460-030, Rio de Janeiro, BrasilLaboratório de Tecido Conjuntivo, Hospital Universitário Clementino Fraga Filho (HUCFF), Instituto de Bioquímica Médica (IBqM), 21941-590, UFRJ, Rio de Janeiro, BrasilLaboratório de Pinças Ópticas-COPEA, ICB/Instituto de Física, 21941-972, UFRJ, Rio de Janeiro, BrasilInstituto de Pesquisas Jardim Botânico do Rio de Janeiro, Diretoria de Pesquisas, MMA, 22460-030, Rio de Janeiro, BrasilLaboratório de Tecido Conjuntivo, HUCFF, IBqM, 21941-590, UFRJ, Rio de Janeiro, BrasilDepartamento de Biologia Marinha, Instituto de Biologia, 24001-970, Universidade Federal Fluminense, Niterói, BrasilLaboratório de Biomineralização, ICB, 21941-590, UFRJ, Rio de Janeiro, BrasilInstituto de Pesquisas Jardim Botânico do Rio de Janeiro, Diretoria de Pesquisas, MMA, 22460-030, Rio de Janeiro, Brasil.

PMID: 27033657 DOI: 10.1111/j.1529-8817.2008.00642.x

Abstract

The interaction between phenolic substances (PS) and alginates (ALG) has been suggested to play a role in the structure of the cell walls of brown seaweeds. However, no clear evidence for this interaction was reported. Vanadium bromoperoxidase (VBPO) has been proposed as a possible catalyst for the binding of PS to ALG. In this work, we studied the interaction between PS and ALG from brown algae using size exclusion chromatography (SEC) and optical tweezers microscopy. The analysis by SEC revealed that ALG forms a high-molecular-weight complex with PS. To study the formation of this molecular complex, we investigated the in vitro interaction of purified ALG from Fucus vesiculosus L. with purified PS from Padina gymnospora (Kütz.) Sond., in the presence or absence of VBPO. The interaction between PS and ALG only occurred when VBPO was added, indicating that the enzyme is essential for the binding process. The interaction of these molecules led to a reduction in ALG viscosity. We propose that VBPO promotes the binding of PS molecules to the ALG uronic acids residues, and we also suggest that PS are components of the brown algal cell walls.

© 2009 Phycological Society of America.

Keywords: binding process; cell wall formation; haloperoxidases; optical tweezers; phenol; phloroglucinol; phlorotannins; polysaccharides; uronic acid; viscosity

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