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Zhou JY, Zheng SR, Liu J, et al. MiR-519d facilitates the progression and metastasis of cervical cancer through direct targeting Smad7. Cancer Cell Int. 2016;16:21doi: 10.1186/s12935-016-0298-1.
Zhou, J. Y., Zheng, S. R., Liu, J., Shi, R., Yu, H. L., & Wei, M. (2016). MiR-519d facilitates the progression and metastasis of cervical cancer through direct targeting Smad7. Cancer cell international, 1621. https://doi.org/10.1186/s12935-016-0298-1
Zhou, Jue-Yu, et al. "MiR-519d facilitates the progression and metastasis of cervical cancer through direct targeting Smad7." Cancer cell international vol. 16 (2016): 21. doi: https://doi.org/10.1186/s12935-016-0298-1
Zhou JY, Zheng SR, Liu J, Shi R, Yu HL, Wei M. MiR-519d facilitates the progression and metastasis of cervical cancer through direct targeting Smad7. Cancer Cell Int. 2016 Mar 22;16:21. doi: 10.1186/s12935-016-0298-1. eCollection 2016. PMID: 27006642; PMCID: PMC4802873.
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Cancer Cell Int. 2016 Mar 22;16:21. doi: 10.1186/s12935-016-0298-1. eCollection 2016.

MiR-519d facilitates the progression and metastasis of cervical cancer through direct targeting Smad7.

Cancer cell international

Jue-Yu Zhou, Si-Rong Zheng, Jie Liu, Rong Shi, Hai-Lang Yu, Min Wei

Affiliations

  1. Institute of Genetic Engineering, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515 Guangdong People's Republic of China.
  2. Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515 Guangdong People's Republic of China.

PMID: 27006642 PMCID: PMC4802873 DOI: 10.1186/s12935-016-0298-1

Abstract

BACKGROUND: MicroRNAs (miRNAs) play pivotal roles in the development of various cancer types, including cervical cancer.

METHODS AND RESULTS: In this study, we showed that miR-519d, a miRNA within the chromosome 19 miRNA cluster, was significantly upregulated in cervical cancer tissues, compared with non-tumorous cervical samples. Suppression of miR-519d markedly attenuated the migration and invasion of HeLa and SiHa cervical cancer cells. Additionally, miR-519d inhibited the apoptosis of cervical cancer cells, and the proliferation of cervical cancer cells was also affected following transfection of miR-519d inhibitor. Moreover, we identified Smad7 to be a novel target of miR-519d in cervical cancer cells. MiR-519d matched the 3'-UTR of Smad7 mRNA. Transfection with miR-519d mimics led to apparent downregulation of Smad7 both at the mRNA and protein levels. Luciferase reporter analysis revealed that miR-519d reduced the luciferase activity of Smad7 mRNA 3'-UTR through matching site-dependent manner. And more notably, suppression of Smad7 remarkably restored the migration and invasion of miR-519d-depleted cervical cancer cells.

CONCLUSION: Taken together, these findings implicated that miR-519d promoted the progression and metastasis of cervical cancer through targeting Smad7.

Keywords: Cervical cancer; Metastasis; MiR-519d; Proliferation; Smad7

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