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Wang CC, Ding S, Chiu KH, et al. Extract from a mutant Rhodobacter sphaeroides as an enriched carotenoid source. Food Nutr Res. 2016;60:29580doi: 10.3402/fnr.v60.29580.
Wang, C. C., Ding, S., Chiu, K. H., Liu, W. S., Lin, T. J., & Wen, Z. H. (2016). Extract from a mutant Rhodobacter sphaeroides as an enriched carotenoid source. Food & nutrition research, 6029580. https://doi.org/10.3402/fnr.v60.29580
Wang, Chih-Chiang, et al. "Extract from a mutant Rhodobacter sphaeroides as an enriched carotenoid source." Food & nutrition research vol. 60 (2016): 29580. doi: https://doi.org/10.3402/fnr.v60.29580
Wang CC, Ding S, Chiu KH, Liu WS, Lin TJ, Wen ZH. Extract from a mutant Rhodobacter sphaeroides as an enriched carotenoid source. Food Nutr Res. 2016 Mar 31;60:29580. doi: 10.3402/fnr.v60.29580. eCollection 2016. PMID: 27037001; PMCID: PMC4818355.
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Food Nutr Res. 2016 Mar 31;60:29580. doi: 10.3402/fnr.v60.29580. eCollection 2016.

Extract from a mutant Rhodobacter sphaeroides as an enriched carotenoid source.

Food & nutrition research

Chih-Chiang Wang, Shangwu Ding, Kuo-Hsun Chiu, Wen-Sheng Liu, Tai-Jung Lin, Zhi-Hong Wen

Affiliations

  1. Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, Taiwan.
  2. Department of Medicine, Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan.
  3. Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan.
  4. Department and Graduate Institute of Aquaculture, National Kaohsiung Marine University, Kaohsiung, Taiwan.
  5. Asia-Pacific Biotech Developing, Inc., Kaohsiung, Taiwan.
  6. Department of Pharmacy and Graduate Institute of Pharmaceutical Technology, Tajen University, Pingtun County, Taiwan.
  7. Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, Taiwan; [email protected].

PMID: 27037001 PMCID: PMC4818355 DOI: 10.3402/fnr.v60.29580

Abstract

BACKGROUND: The extract Lycogen™ from the phototrophic bacterium Rhodobacter sphaeroides (WL-APD911) has attracted significant attention because of its promising potential as a bioactive mixture, attributed in part to its anti-inflammatory properties and anti-oxidative activity.

OBJECTIVE: This study aims to investigate the components of Lycogen™ and its anti-inflammatory properties and anti-oxidative activity.

DESIGN AND RESULTS: The mutant strain R. sphaeroides (WL-APD911) whose carotenoid 1,2-hydratase gene has been altered by chemical mutagenesis was used for the production of a new carotenoid. The strain was grown at 30°C on Luria-Bertani (LB) agar plates. After a 4-day culture period, the mutant strain displayed a 3.5-fold increase in carotenoid content, relative to the wild type. In the DPPH test, Lycogen™ showed more potent anti-oxidative activity than lycopene from the wild-type strain. Primary skin irritation test with hamsters showed no irritation response in hamster skins after 30 days of treatment with 0.2% Lycogen™. Chemical investigations of Lycogen™ using nuclear magnetic resonance (NMR) (1)H, (13)C, and COSY/DQCOSY spectra have identified spheroidenone and methoxyneurosporene. Quantitative analysis of these identified compounds based on spectral intensities indicates that spheroidenone and methoxyneurosporene are major components (approximately 1:1); very small quantities of other derivatives are also present in the sample.

CONCLUSIONS: In this study, we identified the major carotenoid compounds contained in Lycogen™, including spheroidenone and methoxyneurosporene by high-resolution NMR spectroscopy analysis. The carotenoid content of this mutant strain of R. sphaeroides was 3.5-fold higher than that in normal strain. Furthermore, Lycogen™ from the mutant strain is more potent than lycopene from the wild-type strain and does not cause irritation in hamster skins. These findings suggest that this mutant strain has the potential to be used as an enriched carotenoid source.

Keywords: Lycogen™; Rhodobacter sphaeroides; anti-oxidative; carotenoid; methoxyneurosporene; phototrophic bacteria

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