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Lavoie M, Le Faucheur S, Boullemant A, et al. THE INFLUENCE OF pH ON ALGAL CELL MEMBRANE PERMEABILITY AND ITS IMPLICATIONS FOR THE UPTAKE OF LIPOPHILIC METAL COMPLEXES(1). J Phycol. 2012;48(2):293-302doi: 10.1111/j.1529-8817.2012.01126.x.
Lavoie, M., Le Faucheur, S., Boullemant, A., Fortin, C., & Campbell, P. G. (2012). THE INFLUENCE OF pH ON ALGAL CELL MEMBRANE PERMEABILITY AND ITS IMPLICATIONS FOR THE UPTAKE OF LIPOPHILIC METAL COMPLEXES(1). Journal of phycology, 48(2), 293-302. https://doi.org/10.1111/j.1529-8817.2012.01126.x
Lavoie, Michel, et al. "THE INFLUENCE OF pH ON ALGAL CELL MEMBRANE PERMEABILITY AND ITS IMPLICATIONS FOR THE UPTAKE OF LIPOPHILIC METAL COMPLEXES(1)." Journal of phycology vol. 48,2 (2012): 293-302. doi: https://doi.org/10.1111/j.1529-8817.2012.01126.x
Lavoie M, Le Faucheur S, Boullemant A, Fortin C, Campbell PG. THE INFLUENCE OF pH ON ALGAL CELL MEMBRANE PERMEABILITY AND ITS IMPLICATIONS FOR THE UPTAKE OF LIPOPHILIC METAL COMPLEXES(1). J Phycol. 2012 Apr;48(2):293-302. doi: 10.1111/j.1529-8817.2012.01126.x. Epub 2012 Mar 19. PMID: 27009719.
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J Phycol. 2012 Apr;48(2):293-302. doi: 10.1111/j.1529-8817.2012.01126.x. Epub 2012 Mar 19.

THE INFLUENCE OF pH ON ALGAL CELL MEMBRANE PERMEABILITY AND ITS IMPLICATIONS FOR THE UPTAKE OF LIPOPHILIC METAL COMPLEXES(1).

Journal of phycology

Michel Lavoie, Séverine Le Faucheur, Amiel Boullemant, Claude Fortin, Peter G C Campbell

Affiliations

  1. Institut national de la Recherche scientifique - Eau Terre et Environnement (INRS-ETE), 490 de la Couronne, Québec G1K 9A9, Québec, Canada.

PMID: 27009719 DOI: 10.1111/j.1529-8817.2012.01126.x

Abstract

Uptake of lipophilic metal complexes by freshwater algae has recently been shown to be pH dependent. Here we look at different physiological aspects that could influence the diffusion of the lipophilic Cd complex, Cd(diethyldithiocarbamate)2 (0) (Cd(DDC)2 (0) ), into algal cells at different exposure pH values. Changes in cell membrane permeability were assessed as a function of pH for three species of green algae [Chlamydomonas reinhardtii P. A. Dang., Pseudokirchneriella subcapitata (Korshikov) Hindák, and Chlorella fusca var. vacuolata Shihira et R. W. Kraus] using two neutral, nonionic probes, fluorescein diacetate (FDA) and D-sorbitol. In parallel experiments, we exposed algae to inorganic Cd or to Cd(DDC)2 (0) and monitored Cd intracellular metal distribution, together with phytochelatin synthesis. For the three algal species acclimated at pH 5.5 (w/wo DDC 1 μM) and exposed at this pH, their permeability to FDA and D-sorbitol was consistently lower than for algae growing at pH 7.0 and exposed at this pH (P < 0.001). The ratio of the FDA hydrolysis rate measured at pH 7.0 with respect to the rate measured at pH 5.5 (both in the presence of DDC) correlated with the ratio of the Cd(DDC)2 (0) initial internalization rate constant obtained at pH 7.0 versus that obtained at pH 5.5 (three algae species, n = 9, r = 0.85, P = 0.004). Our results strongly suggest that acidification affects metal availability to algae not only by proton inhibition of facilitated metal uptake but also by affecting membrane permeability.

© 2012 Phycological Society of America.

Keywords: Cd; cell membrane; chlorophyte; diethyldithiocarbamate; lipophilic metal complexes; pH; permeability

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