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Anthony JM, Allcock RJ, Krauss SL. Isolation and characterization of 13 microsatellites for the rare endemic shrub Tetratheca erubescens (Elaeocarpaceae). Appl Plant Sci. 2016;4(2)doi: 10.3732/apps.1500102.
Anthony, J. M., Allcock, R. J., & Krauss, S. L. (2016). Isolation and characterization of 13 microsatellites for the rare endemic shrub Tetratheca erubescens (Elaeocarpaceae). Applications in plant sciences, 4(2), . https://doi.org/10.3732/apps.1500102
Anthony, Janet M, et al. "Isolation and characterization of 13 microsatellites for the rare endemic shrub Tetratheca erubescens (Elaeocarpaceae)." Applications in plant sciences vol. 4,2 (2016). doi: https://doi.org/10.3732/apps.1500102
Anthony JM, Allcock RJ, Krauss SL. Isolation and characterization of 13 microsatellites for the rare endemic shrub Tetratheca erubescens (Elaeocarpaceae). Appl Plant Sci. 2016 Feb 09;4(2). doi: 10.3732/apps.1500102. eCollection 2016 Feb. PMID: 26949577; PMCID: PMC4760749.
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Appl Plant Sci. 2016 Feb 09;4(2). doi: 10.3732/apps.1500102. eCollection 2016 Feb.

Isolation and characterization of 13 microsatellites for the rare endemic shrub Tetratheca erubescens (Elaeocarpaceae).

Applications in plant sciences

Janet M Anthony, Richard J N Allcock, Siegfried L Krauss

Affiliations

  1. Kings Park and Botanic Garden, Botanic Garden and Parks Authority, Fraser Avenue, West Perth, 6005 Western Australia, Australia; School of Plant Biology, The University of Western Australia, Nedlands, 6009 Western Australia, Australia.
  2. School of Pathology and Laboratory Medicine, Lotterywest State Biomedical Facility Genomics, University of Western Australia, Crawley, Western Australia 6009, Australia.

PMID: 26949577 PMCID: PMC4760749 DOI: 10.3732/apps.1500102

Abstract

PREMISE OF THE STUDY: Microsatellite markers were developed for the rare Tetratheca erubescens (Elaeocarpaceae) to assess genetic diversity and spatial structuring.

METHODS AND RESULTS: We generated ca. 2.7 million sequence reads using a Personal Genome Machine (PGM) semiconductor sequencer. Using the QDD pipeline, we designed primers for >12,000 sequences with PCR product lengths of 80-480 bp. From these, 30 primer pairs were selected and screened using PCR, from which 11 loci were found to be polymorphic and amplified reliably. For a sample of 95 plants from three populations, the number of alleles observed for these 11 loci ranged from two to seven and expected heterozygosity ranged from 0.06 to 0.72. No consistent evidence for null alleles or departure from Hardy-Weinberg equilibrium was found for any of the 11 loci.

CONCLUSIONS: These markers will enable the quantification of genetic impact of proposed mining activities on the narrow endemic T. erubescens.

Keywords: Elaeocarpaceae; Personal Genome Machine; Tetratheca erubescens; microsatellite primers; shotgun sequencing

References

  1. Bioinformatics. 2012 Oct 1;28(19):2537-9 - PubMed
  2. Mol Ecol Resour. 2009 Jan;9(1):386-9 - PubMed
  3. Theor Appl Genet. 1991 Dec;83(2):194-200 - PubMed
  4. Appl Plant Sci. 2013 Oct 01;1(10):null - PubMed
  5. Mol Ecol Resour. 2014 Nov;14(6):1302-13 - PubMed

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