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Parrello D, Zegeye A, Mustin C, et al. Siderophore-Mediated Iron Dissolution from Nontronites Is Controlled by Mineral Cristallochemistry. Front Microbiol. 2016;7:423doi: 10.3389/fmicb.2016.00423.
Parrello, D., Zegeye, A., Mustin, C., & Billard, P. (2016). Siderophore-Mediated Iron Dissolution from Nontronites Is Controlled by Mineral Cristallochemistry. Frontiers in microbiology, 7423. https://doi.org/10.3389/fmicb.2016.00423
Parrello, Damien, et al. "Siderophore-Mediated Iron Dissolution from Nontronites Is Controlled by Mineral Cristallochemistry." Frontiers in microbiology vol. 7 (2016): 423. doi: https://doi.org/10.3389/fmicb.2016.00423
Parrello D, Zegeye A, Mustin C, Billard P. Siderophore-Mediated Iron Dissolution from Nontronites Is Controlled by Mineral Cristallochemistry. Front Microbiol. 2016 Mar 31;7:423. doi: 10.3389/fmicb.2016.00423. eCollection 2016. PMID: 27064911; PMCID: PMC4814481.
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Front Microbiol. 2016 Mar 31;7:423. doi: 10.3389/fmicb.2016.00423. eCollection 2016.

Siderophore-Mediated Iron Dissolution from Nontronites Is Controlled by Mineral Cristallochemistry.

Frontiers in microbiology

Damien Parrello, Asfaw Zegeye, Christian Mustin, Patrick Billard

Affiliations

  1. Laboratoire Interdisciplinaire des Environnements Continentaux, UMR 7360 Centre National de la Recherche Scientifique - Université de LorraineVandœuvre-lès-Nancy, France; Civil and Environmental Engineering, University of MissouriColumbia, MO, USA.
  2. Laboratoire Interdisciplinaire des Environnements Continentaux, UMR 7360 Centre National de la Recherche Scientifique - Université de Lorraine Vandœuvre-lès-Nancy, France.

PMID: 27064911 PMCID: PMC4814481 DOI: 10.3389/fmicb.2016.00423

Abstract

Bacteria living in oxic environments experience iron deficiency due to limited solubility and slow dissolution kinetics of iron-bearing minerals. To cope with iron deprivation, aerobic bacteria have evolved various strategies, including release of siderophores or other organic acids that scavenge external Fe(III) and deliver it to the cells. This research investigated the role of siderophores produced by Pseudomonas aeruginosa in the acquisition of Fe(III) from two iron-bearing colloidal nontronites (NAu-1 and NAu-2), comparing differences in bioavailability related with site occupancy and distribution of Fe(III) in the two lattices. To avoid both the direct contact of the mineral colloids with the bacterial cells and the uncontrolled particle aggregation, nontronite suspensions were homogenously dispersed in a porous silica gel before the dissolution experiments. A multiparametric approach coupling UV-vis spectroscopy and spectral decomposition algorithm was implemented to monitor simultaneously the solubilisation of Fe and the production of pyoverdine in microplate-based batch experiments. Both nontronites released Fe in a particle concentration-dependent manner when incubated with the wild-type P. aeruginosa strain, however iron released from NAu-2 was substantially greater than from NAu-1. The profile of organic acids produced in both cases was similar and may not account for the difference in the iron dissolution efficiency. In contrast, a pyoverdine-deficient mutant was unable to mobilize Fe(III) from either nontronite, whereas iron dissolution occurred in abiotic experiments conducted with purified pyoverdine. Overall, our data provide evidence that P. aeruginosa indirectly mobilize Fe from nontronites primarily through the production of pyoverdine. The structural Fe present on the edges of NAu-2 rather than NAu-1 particles appears to be more bio-accessible, indicating that the distribution of Fe, in the tetrahedron and/or in the octahedron sites, governs the solubilisation process. Furthermore, we also revealed that P. aeruginosa could acquire iron when in direct contact with mineral particles in a siderophore-independent manner.

Keywords: Pseudomonas aeruginosa; bioweathering; clay; iron mobilization; nontronite; pyoverdine; siderophore

References

  1. Appl Environ Microbiol. 1992 Sep;58(9):2886-93 - PubMed
  2. Environ Sci Technol. 2015 Aug 18;49(16):10217-25 - PubMed
  3. FEMS Microbiol Rev. 2003 Jun;27(2-3):215-37 - PubMed
  4. PLoS One. 2015 Mar 30;10(3):e0122848 - PubMed
  5. Met Ions Biol Syst. 1998;35:239-327 - PubMed
  6. Environ Microbiol. 2013 Jun;15(6):1661-73 - PubMed
  7. Mol Plant Microbe Interact. 2000 Nov;13(11):1243-50 - PubMed
  8. Environ Microbiol Rep. 2014 Oct;6(5):459-67 - PubMed
  9. Mol Microbiol. 2002 Sep;45(5):1277-87 - PubMed
  10. Microbiology. 2004 Jun;150(Pt 6):1671-80 - PubMed
  11. Nat Prod Rep. 2010 May;27(5):637-57 - PubMed
  12. Environ Sci Technol. 2011 Feb 1;45(3):977-83 - PubMed
  13. J Colloid Interface Sci. 2010 Mar 15;343(2):433-8 - PubMed
  14. Langmuir. 2008 Apr 1;24(7):3127-39 - PubMed
  15. J Colloid Interface Sci. 2011 Oct 15;362(2):317-24 - PubMed
  16. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4256-60 - PubMed
  17. J Basic Microbiol. 2013 Apr;53(4):303-17 - PubMed
  18. Appl Environ Microbiol. 2010 Apr;76(7):2041-8 - PubMed
  19. FEMS Microbiol Lett. 2014 May;354(1):37-45 - PubMed
  20. Dalton Trans. 2012 Mar 7;41(9):2820-34 - PubMed

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