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Torres J, Rivera A, Clark G, et al. PARTICIPATION OF EXTRACELLULAR NUCLEOTIDES IN THE WOUND RESPONSE OF DASYCLADUS VERMICULARIS AND ACETABULARIA ACETABULUM (DASYCLADALES, CHLOROPHYTA)(1). J Phycol. 2008;44(6):1504-11doi: 10.1111/j.1529-8817.2008.00602.x.
Torres, J., Rivera, A., Clark, G., & Roux, S. J. (2008). PARTICIPATION OF EXTRACELLULAR NUCLEOTIDES IN THE WOUND RESPONSE OF DASYCLADUS VERMICULARIS AND ACETABULARIA ACETABULUM (DASYCLADALES, CHLOROPHYTA)(1). Journal of phycology, 44(6), 1504-11. https://doi.org/10.1111/j.1529-8817.2008.00602.x
Torres, Jonathan, et al. "PARTICIPATION OF EXTRACELLULAR NUCLEOTIDES IN THE WOUND RESPONSE OF DASYCLADUS VERMICULARIS AND ACETABULARIA ACETABULUM (DASYCLADALES, CHLOROPHYTA)(1)." Journal of phycology vol. 44,6 (2008): 1504-11. doi: https://doi.org/10.1111/j.1529-8817.2008.00602.x
Torres J, Rivera A, Clark G, Roux SJ. PARTICIPATION OF EXTRACELLULAR NUCLEOTIDES IN THE WOUND RESPONSE OF DASYCLADUS VERMICULARIS AND ACETABULARIA ACETABULUM (DASYCLADALES, CHLOROPHYTA)(1). J Phycol. 2008 Dec;44(6):1504-11. doi: 10.1111/j.1529-8817.2008.00602.x. PMID: 27039864.
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J Phycol. 2008 Dec;44(6):1504-11. doi: 10.1111/j.1529-8817.2008.00602.x.

PARTICIPATION OF EXTRACELLULAR NUCLEOTIDES IN THE WOUND RESPONSE OF DASYCLADUS VERMICULARIS AND ACETABULARIA ACETABULUM (DASYCLADALES, CHLOROPHYTA)(1).

Journal of phycology

Jonathan Torres, Amy Rivera, Greg Clark, Stanley J Roux

Affiliations

  1. Section of Molecular Cell and Developmental Biology, University of Texas, Austin, Texas 78712, USA.

PMID: 27039864 DOI: 10.1111/j.1529-8817.2008.00602.x

Abstract

As assayed by fluorescent reporter dyes, nitric oxide (NO) and H2 O2 , two downstream signaling agents induced by wounding in the alga Dasycladus vermicularis (Scop.) Krasser, can also be induced in unwounded Dasycladus cells by μM Adenosine 5'[γ-thio]triphosphate (ATPγS) and Adenosine 5'-[β-thio]diphosphate (ADPβS), but not by Adenosine 5'-O-thiomonophosphate (AMPS). These nucleotide-induced responses are blocked by pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS), an antagonist of animal purinoceptors, and by adenosine, a feed-back inhibitor of extracellular nucleotide responses in animals. Similar nucleotide- and nucleotide-antagonist responses were observed in Acetabularia acetabulum (L.) P. C. Silva. Significant levels of ATP released from Dasycladus cells were measured at wound sites by a sensitive luciferin-luciferase assay. Additionally, the normal wound-induced production of NO and H2 O2 in Dasycladus can be blocked by pretreating the cells with PPADS. Our results indicate that nucleotides released from wounds can serve as a signal to trigger wound responses in algae, and that coordinated signaling between extracellular nucleotides and the NO pathway may have been established early during the evolution of plants.

© 2008 Phycological Society of America.

Keywords: Acetabularia acetabulum; Dasycladus vermicularis; extracellular ATP; hydrogen peroxide; macroalgae; nitric oxide; purinoceptor antagonist; wounding

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