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Vijayaraghavan P, Arun A, Vincent SG, et al. Cow Dung Is a Novel Feedstock for Fibrinolytic Enzyme Production from Newly Isolated Bacillus sp. IND7 and Its Application in In Vitro Clot Lysis. Front Microbiol. 2016;7:361doi: 10.3389/fmicb.2016.00361.
Vijayaraghavan, P., Arun, A., Vincent, S. G., Arasu, M. V., & Al-Dhabi, N. A. (2016). Cow Dung Is a Novel Feedstock for Fibrinolytic Enzyme Production from Newly Isolated Bacillus sp. IND7 and Its Application in In Vitro Clot Lysis. Frontiers in microbiology, 7361. https://doi.org/10.3389/fmicb.2016.00361
Vijayaraghavan, Ponnuswamy, et al. "Cow Dung Is a Novel Feedstock for Fibrinolytic Enzyme Production from Newly Isolated Bacillus sp. IND7 and Its Application in In Vitro Clot Lysis." Frontiers in microbiology vol. 7 (2016): 361. doi: https://doi.org/10.3389/fmicb.2016.00361
Vijayaraghavan P, Arun A, Vincent SG, Arasu MV, Al-Dhabi NA. Cow Dung Is a Novel Feedstock for Fibrinolytic Enzyme Production from Newly Isolated Bacillus sp. IND7 and Its Application in In Vitro Clot Lysis. Front Microbiol. 2016 Mar 29;7:361. doi: 10.3389/fmicb.2016.00361. eCollection 2016. PMID: 27065952; PMCID: PMC4810022.
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Front Microbiol. 2016 Mar 29;7:361. doi: 10.3389/fmicb.2016.00361. eCollection 2016.

Cow Dung Is a Novel Feedstock for Fibrinolytic Enzyme Production from Newly Isolated Bacillus sp. IND7 and Its Application in In Vitro Clot Lysis.

Frontiers in microbiology

Ponnuswamy Vijayaraghavan, Arumugaperumal Arun, Samuel Gnana Prakash Vincent, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi

Affiliations

  1. International Centre for Nanobiotechnology, Centre for Marine Science and Technology, Manonmaniam Sundaranar University Rajakkamangalam, India.
  2. Department of Biotechnology, Kalasalingam University Srivilliputtur, India.
  3. Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University Riyadh, Saudi Arabia.

PMID: 27065952 PMCID: PMC4810022 DOI: 10.3389/fmicb.2016.00361

Abstract

Bacterial fibrinolytic enzymes find great applications to treat and prevent cardiovascular diseases. The novel fibrinolytic enzymes from food grade organisms are useful for thrombolytic therapy. This study reports fibrinolytic enzyme production by Bacillus sp. IND7 in solid-state fermentation (SSF). In this study, cow dung was used as the cheap substrate for the production of fibrinolytic enzyme. Enzyme production was primarily improved by optimizing the nutrient and physical factors by one-variable-at-a-time approach. A statistical method (two-level full factorial design) was applied to investigate the significant variables. Of the different variables, pH, starch, and beef extract significantly influenced on the production of fibrinolytic enzyme (p < 0.05). The optimum levels of these significant factors were further investigated using response surface methodology. The optimum conditions for enhanced fibrinolytic enzyme production were 1.23% (w/w) starch and 0.3% (w/w) beef extract with initial medium pH 9.0. Under the optimized conditions, cow dung substrate yielded 8,345 U/g substrate, and an overall 2.5-fold improvement in fibrinolytic enzyme production was achieved due to its optimization. This is the first report of fibrinolytic enzyme production using cow dung substrate from Bacillus sp. in SSF. The crude enzyme displayed potent activity on zymography and digested goat blood clot completely in in vitro condition.

Keywords: Bacillus sp. IND7; agroresidues; cow dung; fibrinolytic enzyme; response surface methodology; solid-state fermentation

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