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Fazeli Z, Ghaderian SM, Rajabibazl M, et al. Expression Pattern of Neuronal Markers in PB-MSCs Treated by Growth Factors Noggin, bFGF and EGF. Int J Mol Cell Med. 2015;4(4):209-17
Fazeli, Z., Ghaderian, S. M., Rajabibazl, M., Salami, S., Vazifeh Shiran, N., & Omrani, M. D. (2015). Expression Pattern of Neuronal Markers in PB-MSCs Treated by Growth Factors Noggin, bFGF and EGF. International journal of molecular and cellular medicine, 4(4), 209-17.
Fazeli, Zahra, et al. "Expression Pattern of Neuronal Markers in PB-MSCs Treated by Growth Factors Noggin, bFGF and EGF." International journal of molecular and cellular medicine vol. 4,4 (2015): 209-17.
Fazeli Z, Ghaderian SM, Rajabibazl M, Salami S, Vazifeh Shiran N, Omrani MD. Expression Pattern of Neuronal Markers in PB-MSCs Treated by Growth Factors Noggin, bFGF and EGF. Int J Mol Cell Med. 2015;4(4):209-17. PMID: 27014645; PMCID: PMC4769598.
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Int J Mol Cell Med. 2015;4(4):209-17.

Expression Pattern of Neuronal Markers in PB-MSCs Treated by Growth Factors Noggin, bFGF and EGF.

International journal of molecular and cellular medicine

Zahra Fazeli, Sayyed Mohammad Hossein Ghaderian, Masoumeh Rajabibazl, Siamak Salami, Nader Vazifeh Shiran, Mir Davood Omrani

Affiliations

  1. Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  2. Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.; Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Shahid Labbafi Nejad Educational Hospital, Tehran, Iran.
  3. Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  4. Department of Hematology, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

PMID: 27014645 PMCID: PMC4769598

Abstract

Mesenchymal stem cells (MSCs) have the ability to differentiate into neuronal like cells under appropriate culture condition. In this study, we investigated whether MSCs derived from human peripheral blood (PB-MSCs) can differentiate into neuronal like cells by synergic effect of the growth factors EGF, bFGF and Noggin. For this purpose, the expression of five neuronal markers (Nestin, β III tubulin, NFM, MAP2 and NSE) were evaluated in treated PB-MSCs by SYBR Green Real time PCR. The expression analysis showed a higher expression of β-tubulin and NFM in treated BP-MSCs compared with untreated PB-MSCs as a control group. The expression of Nestin was also diminished in PB-MSCs treated with Noggin. This study suggested that the treatment of PB- MSCs with Noggin alongside with bFGF and EGF might differentiate these cells into neuronal lineage cells. The obtained results could be further developed for useful applications in regenerative medicine.

Keywords: Mesenchymal stem cells; Noggin; differentiation; neuronal markers

References

  1. J Neurochem. 1978 Sep;31(3):727-32 - PubMed
  2. Bone. 2009 Aug;45(2):254-66 - PubMed
  3. Stem Cell Res. 2015 Mar;14(2):185-97 - PubMed
  4. Nature. 2011 Mar 3;471(7336):63-7 - PubMed
  5. J Biomed Sci. 2004 Sep-Oct;11(5):652-60 - PubMed
  6. Development. 2005 Jan;132(2):299-310 - PubMed
  7. J Biomed Biotechnol. 2012;2012:820821 - PubMed
  8. Curr Med Chem. 2012;19(35):6018-35 - PubMed
  9. J Biol Chem. 2000 Mar 31;275(13):9645-52 - PubMed
  10. Exp Hematol. 2006 Nov;34(11):1563-72 - PubMed
  11. J Neurosci Res. 2006 May 1;83(6):1015-27 - PubMed
  12. Cytotherapy. 2009;11(2):163-76 - PubMed
  13. Hum Cell. 2014 Apr;27(2):51-8 - PubMed
  14. Cell Mol Biol Lett. 2013 Jun;18(2):163-86 - PubMed
  15. Hum Mol Genet. 2008 Apr 15;17(R1):R60-6 - PubMed
  16. Yonsei Med J. 2011 May;52(3):401-12 - PubMed
  17. J Orthop Res. 2013 Nov;31(11):1796-803 - PubMed
  18. J Histochem Cytochem. 2008 Dec;56(12):1113-9 - PubMed
  19. Bone. 2007 May;40(5):1389-98 - PubMed
  20. Stem Cells. 2005 Mar;23(3):392-402 - PubMed
  21. J Neurochem. 1996 Mar;66(3):1273-81 - PubMed
  22. Nucleic Acids Res. 2001 May 1;29(9):e45 - PubMed
  23. Cell Biol Int. 2011 Jun;35(6):631-8 - PubMed
  24. Stem Cells. 2004;22(3):377-84 - PubMed
  25. Neurol Res Int. 2014;2014:807896 - PubMed
  26. J Neurosci Res. 1995 Dec 15;42(6):803-817 - PubMed
  27. J Biomed Sci. 2014 Sep 24;21:83 - PubMed
  28. Stem Cells. 2014 Nov;32(11):2811-7 - PubMed
  29. J Mol Med (Berl). 1997 Sep;75(9):653-63 - PubMed
  30. Exp Cell Res. 2001 Aug 15;268(2):189-200 - PubMed
  31. Mol Cancer. 2006 Dec 02;5:67 - PubMed
  32. J Orthop Res. 2012 Apr;30(4):634-42 - PubMed
  33. Eur Cytokine Netw. 2014 Jul-Sep;25(3):58-63 - PubMed
  34. BMC Vet Res. 2014 Aug 05;10:173 - PubMed
  35. Stem Cells. 2006 Jan;24(1):115-24 - PubMed
  36. J Neural Transm Suppl. 2007;(72):17-28 - PubMed
  37. Neuroreport. 2010 Feb 17;21(3):185-90 - PubMed
  38. Biochem Genet. 2013 Apr;51(3-4):175-88 - PubMed
  39. Cell. 1990 Feb 23;60(4):585-95 - PubMed
  40. J Neurochem. 2005 Mar;92(5):1265-76 - PubMed
  41. Mol Neurobiol. 1998 Apr;16(2):149-62 - PubMed
  42. Stem Cells. 2006 Aug;24(8):1841-51 - PubMed
  43. Nat Rev Mol Cell Biol. 2011 Feb;12(2):79-89 - PubMed
  44. DNA Cell Biol. 2013 Oct;32(10):565-72 - PubMed

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