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Fei Y, Xiao B, Yang M, et al. MicroRNAs, polyamines, and the activities antioxidant enzymes are associated with in vitro rooting in white pine (Pinus strobus L.). Springerplus. 2016;5:416doi: 10.1186/s40064-016-2080-1.
Fei, Y., Xiao, B., Yang, M., Ding, Q., & Tang, W. (2016). MicroRNAs, polyamines, and the activities antioxidant enzymes are associated with in vitro rooting in white pine (Pinus strobus L.). SpringerPlus, 5416. https://doi.org/10.1186/s40064-016-2080-1
Fei, Yunjun, et al. "MicroRNAs, polyamines, and the activities antioxidant enzymes are associated with in vitro rooting in white pine (Pinus strobus L.)." SpringerPlus vol. 5 (2016): 416. doi: https://doi.org/10.1186/s40064-016-2080-1
Fei Y, Xiao B, Yang M, Ding Q, Tang W. MicroRNAs, polyamines, and the activities antioxidant enzymes are associated with in vitro rooting in white pine (Pinus strobus L.). Springerplus. 2016 Apr 06;5:416. doi: 10.1186/s40064-016-2080-1. eCollection 2016. PMID: 27069836; PMCID: PMC4821849.
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Springerplus. 2016 Apr 06;5:416. doi: 10.1186/s40064-016-2080-1. eCollection 2016.

MicroRNAs, polyamines, and the activities antioxidant enzymes are associated with in vitro rooting in white pine (Pinus strobus L.).

SpringerPlus

Yunjun Fei, Bo Xiao, Man Yang, Qiong Ding, Wei Tang

Affiliations

  1. College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025 Hubei China.
  2. College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025 Hubei China ; Institute for Genome Sciences and Policy, Duke University, Durham, NC 27708 USA.

PMID: 27069836 PMCID: PMC4821849 DOI: 10.1186/s40064-016-2080-1

Abstract

Molecular mechanism of in vitro rooting in conifer is not fully understood. After establishment of a regeneration procedure in eastern white pine (Pinus strobus L.) using mature embryos as explants to induce shoot formation on medium containing 3 μM IAA, 6 μM BA and 6 μM TDZ and induce root formation on medium containing 0.001-0.05 μM IAA, 0.001-0.05 μM IBA, 0.001-0.05 μM TDZ, we have investigated the changes of polyamine content and the activities of antioxidant enzymes during in vitro rooting in P. strobus. Our results demonstrated that putrescine (Put), spermidine (Spd), and spermine (Spm) did not increase in P. strobus during the first week of rooting on medium supplemented with 0.01 μM indole-3-acetic acid (IAA), whereas the levels of Put, Spd, and Spm increased during the 1st-3rd week of culture on medium with IAA, and then decreased on medium with IAA. No such a change in Put, Spd, and Spm was observed on medium without IAA. Measurement of antioxidant enzyme activity demonstrated that the activities of polyphenol oxidase, catalase, and peroxidase slightly increased in the first week of culture and reached to the highest peak in the 3rd-5th week of culture. Quantitative RT-PCR results indicated that miR160 was increased on the 7th day, miR162, miR397, and miR408 was increased from the 21th to 35th day, miR857 was increased on the 35th day, and miR827 was increased on the 49th day. These results demonstrated that enhanced polyamine biosynthesis, antioxidant enzyme activity, and microRNAs are correlated with the root induction and formation in P. strobus.

Keywords: Antioxidant enzyme; MicroRNAs; Pinus strobus; Polyamines; Root formation

References

  1. Plant Physiol Biochem. 2004 Jan;42(1):35-42 - PubMed
  2. Plant Biotechnol Rep. 2012 Jan;6(1):1-15 - PubMed
  3. Proc Natl Acad Sci U S A. 2008 Jan 15;105(2):803-8 - PubMed
  4. Proc Natl Acad Sci U S A. 2007 Dec 26;104(52):20649-50 - PubMed
  5. Plant Cell Rep. 1988 May;7(3):174-7 - PubMed
  6. J Chem Ecol. 2011 May;37(5):460-71 - PubMed
  7. Plant Biotechnol Rep. 2012 Jan;6(1):39-46 - PubMed
  8. Plant Cell Rep. 2005 Dec;24(10):581-9 - PubMed
  9. Plant Physiol. 1982 Mar;69(3):701-6 - PubMed
  10. BMC Plant Biol. 2013 Jan 15;13:10 - PubMed
  11. J Plant Physiol. 2013 Jan 15;170(2):185-95 - PubMed
  12. Planta. 2013 Aug;238(2):271-82 - PubMed
  13. Genes Dev. 2001 Jun 15;15(12):1577-88 - PubMed
  14. PLoS Genet. 2009 Nov;5(11):e1000716 - PubMed
  15. Plant Cell. 2009 Oct;21(10):3119-32 - PubMed
  16. J Biol Chem. 1952 Mar;195(1):133-40 - PubMed
  17. Planta. 2013 Sep;238(3):499-517 - PubMed
  18. Biotechnol Adv. 2000 Apr;18(2):91-120 - PubMed
  19. Science. 1982 Sep 24;217(4566):1259-61 - PubMed
  20. Plant Cell. 2012 Jun;24(6):2515-27 - PubMed
  21. Plant Cell Rep. 2005 Apr;24(1):1-9 - PubMed
  22. Plant Biotechnol Rep. 2013;7:547-555 - PubMed
  23. Plant Cell Rep. 2003 Aug;22(1):1-15 - PubMed
  24. J Exp Bot. 2006;57(8):1829-34 - PubMed
  25. Biochem Biophys Res Commun. 2010 Mar 12;393(3):345-9 - PubMed
  26. Sci Rep. 2015 Jul 02;5:11813 - PubMed
  27. Plant Cell. 2005 May;17(5):1360-75 - PubMed
  28. Plant Cell Rep. 1987 Feb;6(1):5-7 - PubMed
  29. Plant Physiol Biochem. 2005 Aug;43(8):760-9 - PubMed
  30. Genes Dev. 1991 Mar;5(3):438-46 - PubMed

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