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Antebi U, Mathor MB, da Silva AF, et al. Effects of ionizing radiation on proteins in lyophilized or frozen demineralized human bone. Rev Bras Ortop. 2016;51(2):224-30doi: 10.1016/j.rboe.2016.02.006.
Antebi, U., Mathor, M. B., da Silva, A. F., Guimarães, R. P., & Honda, E. K. (2016). Effects of ionizing radiation on proteins in lyophilized or frozen demineralized human bone. Revista brasileira de ortopedia, 51(2), 224-30. https://doi.org/10.1016/j.rboe.2016.02.006
Antebi, Uri, et al. "Effects of ionizing radiation on proteins in lyophilized or frozen demineralized human bone." Revista brasileira de ortopedia vol. 51,2 (2016): 224-30. doi: https://doi.org/10.1016/j.rboe.2016.02.006
Antebi U, Mathor MB, da Silva AF, Guimarães RP, Honda EK. Effects of ionizing radiation on proteins in lyophilized or frozen demineralized human bone. Rev Bras Ortop. 2016 Feb 23;51(2):224-30. doi: 10.1016/j.rboe.2016.02.006. eCollection 2016. PMID: 27069893; PMCID: PMC4812029.
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Rev Bras Ortop. 2016 Feb 23;51(2):224-30. doi: 10.1016/j.rboe.2016.02.006. eCollection 2016.

Effects of ionizing radiation on proteins in lyophilized or frozen demineralized human bone.

Revista brasileira de ortopedia

Uri Antebi, Monica Beatriz Mathor, André Ferreira da Silva, Rodrigo Pereira Guimarães, Emerson Kiyoshi Honda

Affiliations

  1. Irmandade da Santa Casa de Misericórdia de São Paulo, São Paulo, SP, Brazil; Instituto de Pesquisas Energéticas e Nucleares (IPEN), São Paulo, SP, Brazil.
  2. Instituto de Pesquisas Energéticas e Nucleares (IPEN), São Paulo, SP, Brazil.
  3. Instituto Paulista de Cirurgia do Quadril e Joelho, São Paulo, SP, Brazil; Universidade Nove de Julho, São Paulo, SP, Brazil.
  4. Faculdade de Ciências Médicas da Santa Casa de São Paulo, São Paulo, SP, Brazil.

PMID: 27069893 PMCID: PMC4812029 DOI: 10.1016/j.rboe.2016.02.006

Abstract

OBJECTIVE: The aim was to study the effects of application of ionizing radiation (gamma and electrons) as sterilizing agents at doses of 15 kGy, 25 kGy and 50 kGy, on lyophilized or frozen demineralized bone tissue for use in transplants.

METHODS: Five human femoral diaphyses from different donors of musculoskeletal tissue were demineralized and preserved as lyophilized or frozen at -80 °C. The samples were divided into two groups: non-irradiated (control) and irradiated by means of gamma rays or an electron beam. The bone proteins were extracted and used to determine the concentrations of total protein and BMP 2 and 7.

RESULTS: Decreases in total protein and BMP 2 and 7 concentrations were observed. The decreases in total protein concentrations, in comparison with the respective control groups, were significant in the lyophilized and frozen samples that were irradiated at a dose of 50 kGy of gamma radiation and electron beam, with reductions of more than 30%. Significant decreases in the levels of BMP 2 and 7 were also observed at higher doses and especially through use of the electron beam.

CONCLUSION: The reductions in the concentrations of total proteins and osteoinductive proteins (BMP 2 and 7) were related to the radiation dose, i.e. they increased with higher doses of ionizing radiation type and the type of bone preservation. The largest reductions in concentrations were observed in the bones irradiated by means of an electron beam and at a dose of 50 kGy. However, this type of radiation and this high dose are not usual practices for sterilization of bone tissue.

Keywords: Bone and bones; Bone morphogenetic protein 2; Bone morphogenetic protein 7; Radiation, ionizing; Tissue banks

References

  1. Growth Factors. 1996;13(3-4):273-89,color plates III-VIII,pre.bk - PubMed
  2. Orthopedics. 2004 Jan;27(1 Suppl):s161-5 - PubMed
  3. Scand J Surg. 2005;94(1):67-70 - PubMed
  4. Proc Natl Acad Sci U S A. 1984 Jan;81(2):371-5 - PubMed
  5. Cell Tissue Bank. 2011 May;12(2):81-8 - PubMed
  6. Hand Clin. 2005 Nov;21(4):591-601 - PubMed
  7. Calcif Tissue Int. 1988 Apr;42(4):255-60 - PubMed
  8. Clin Orthop Relat Res. 1991 Nov;(272):30-7 - PubMed
  9. Transfusion. 1993 Aug;33(8):679-85 - PubMed
  10. Cell Tissue Bank. 2007;8(2):93-105 - PubMed
  11. Acta Orthop Scand. 1988 Feb;59(1):34-8 - PubMed

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