Display options
Cite
Shahjouei S, Cai PY, Ansari S, et al. Middle Cerebral Artery Occlusion Model of Stroke in Rodents: A Step-by-Step Approach. J Vasc Interv Neurol. 2016;8(5):1-8
Shahjouei, S., Cai, P. Y., Ansari, S., Sharififar, S., Azari, H., Ganji, S., & Zand, R. (2016). Middle Cerebral Artery Occlusion Model of Stroke in Rodents: A Step-by-Step Approach. Journal of vascular and interventional neurology, 8(5), 1-8.
Shahjouei, Shima, et al. "Middle Cerebral Artery Occlusion Model of Stroke in Rodents: A Step-by-Step Approach." Journal of vascular and interventional neurology vol. 8,5 (2016): 1-8.
Shahjouei S, Cai PY, Ansari S, Sharififar S, Azari H, Ganji S, Zand R. Middle Cerebral Artery Occlusion Model of Stroke in Rodents: A Step-by-Step Approach. J Vasc Interv Neurol. 2016 Jan;8(5):1-8. PMID: 26958146; PMCID: PMC4762402.
Copy Download .nbib Format: NLM
Share it on

J Vasc Interv Neurol. 2016 Jan;8(5):1-8.

Middle Cerebral Artery Occlusion Model of Stroke in Rodents: A Step-by-Step Approach.

Journal of vascular and interventional neurology

Shima Shahjouei, Peter Y Cai, Saeed Ansari, Sharareh Sharififar, Hassan Azari, Sarah Ganji, Ramin Zand

Affiliations

  1. These coauthors are considered as first author in this study; Department of Neurosurgery, Tehran University of Medical Sciences, Tehran, Iran.
  2. These coauthors are considered as first author in this study; Department of Neurology, University of Florida, Gainesville, FL, USA.
  3. Department of Neurology, University of Tennessee Health Sciences Center, Memphis, TN, USA.
  4. Department of Physical Therapy, University of Florida, Gainesville, FL, USA.
  5. Department of Neurosurgery, University of Florida, Gainesville, FL, USA; Department of Anatomical Sciences, Neural Stem Cell and Regenerative Neuroscience Laboratory, Shiraz University of Medical Sciences, Shiraz, Iran.
  6. Department of Pediatrics, Mercer University School of Medicine, Macon, GA, USA.

PMID: 26958146 PMCID: PMC4762402

Abstract

Stroke is one of the leading causes of morbidity and mortality in developed countries and an immense amount of medical care resources are devoted to combat the poststroke debilitating consequences. The key to develop effective and clinically applicable treatment methodologies is a better understanding of the pathophysiology of the disease, including the root causes and targets for pharmacology. Developing these foundations requires the use of standard animal models that mimic the physicochemical process of the diseases that can reliably replicate results in order to test and fine-tune therapeutic modalities. Middle cerebral artery occlusion (MCAO), endothelin-1-induced ischemic stroke, photothrombosis, devascularization, embolization, and spontaneous infarction using hemorrhage are some examples of different animal models. Reliability of MCAO has been proved and due to the ability to induce reperfusion similar to tissue plasminogen activator (tPA) therapy, this model is widely used in preclinical studies. Here, we describe a detailed methodology on how to develop MCAO stroke in rodents using intra-arterial insertion of filament to occlude the middle cerebral artery. This approach allows for the study of a wide array of basic pathophysiology mechanisms, regenerative medicine and rehabilitation therapy.

Keywords: Animal model; middle cerebral artery; occlusion; stroke

References

  1. J Biomed Biotechnol. 2011;2011:464701 - PubMed
  2. Stroke. 2009 Feb;40(2):510-5 - PubMed
  3. Stroke. 1989 Jan;20(1):84-91 - PubMed
  4. Circulation. 2009 Jan 27;119(3):e21-181 - PubMed
  5. J Neuroimmunol. 2007 Mar;184(1-2):53-68 - PubMed
  6. Stroke. 2011 Sep;42(9):2645-50 - PubMed
  7. BMJ. 2009 Nov 11;339:b4584 - PubMed
  8. Front Neurol. 2014 Feb 19;5:19 - PubMed
  9. Circulation. 2014 Jan 21;129(3):399-410 - PubMed
  10. J Stroke Cerebrovasc Dis. 2003 May-Jun;12(3):119-26 - PubMed
  11. Circulation. 2012 Jan 3;125(1):188-97 - PubMed
  12. Stroke. 1999 Dec;30(12):2752-8 - PubMed

Publication Types