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Kawata Y, Nishimura T, Matsushita I, et al. Efficient production and secretion of pyruvate from Halomonas sp. KM-1 under aerobic conditions. AMB Express. 2016;6(1):22doi: 10.1186/s13568-016-0195-y.
Kawata, Y., Nishimura, T., Matsushita, I., & Tsubota, J. (2016). Efficient production and secretion of pyruvate from Halomonas sp. KM-1 under aerobic conditions. AMB Express, 6(1), 22. https://doi.org/10.1186/s13568-016-0195-y
Kawata, Yoshikazu, et al. "Efficient production and secretion of pyruvate from Halomonas sp. KM-1 under aerobic conditions." AMB Express vol. 6,1 (2016): 22. doi: https://doi.org/10.1186/s13568-016-0195-y
Kawata Y, Nishimura T, Matsushita I, Tsubota J. Efficient production and secretion of pyruvate from Halomonas sp. KM-1 under aerobic conditions. AMB Express. 2016 Mar;6(1):22. doi: 10.1186/s13568-016-0195-y. Epub 2016 Mar 18. PMID: 26989057; PMCID: PMC4798600.
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AMB Express. 2016 Mar;6(1):22. doi: 10.1186/s13568-016-0195-y. Epub 2016 Mar 18.

Efficient production and secretion of pyruvate from Halomonas sp. KM-1 under aerobic conditions.

AMB Express

Yoshikazu Kawata, Taku Nishimura, Isao Matsushita, Jun Tsubota

Affiliations

  1. Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka, 563-8577, Japan. [email protected].
  2. Energy Technology Laboratories, OSAKA GAS Co., Ltd., 6-19-9 Torishima, Konohana-ku, Osaka, 554-0051, Japan.

PMID: 26989057 PMCID: PMC4798600 DOI: 10.1186/s13568-016-0195-y

Abstract

The alkaliphilic, halophilic bacterium Halomonas sp. KM-1 can utilize both hexose and pentose sugars for the intracellular storage of bioplastic poly-(R)-3-hydroxybutyric acid (PHB) under aerobic conditions. In this study, we investigated the effects of the sodium nitrate concentration on PHB accumulation in the KM-1 strain. Unexpectedly, we observed the secretion of pyruvate, a central intermediate in carbon- and energy-metabolism processes in all organisms; therefore, pyruvate is widely used as a starting material in the industrial biosynthesis of pharmaceuticals and is employed for the production of crop-protection agents, polymers, cosmetics, and food additives. We then further analyzed pyruvate productivity following changes in culture temperature and the buffer concentration. In 48-h batch-cultivation experiments, we found that wild-type Halomonas sp. KM-1 secreted 63.3 g/L pyruvate at a rate of 1.32 g/(L·h), comparable to the results of former studies using mutant and recombinant microorganisms. Thus, these data provided important insights into the production of pyruvate using this novel strain.

Keywords: Halomonas; Nitrate; Poly-(R)-3-hydroxybutyric acid; Pyruvate

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