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Sadhu DB, Panchasara HH, Chauhan HC, et al. Seroprevalence and comparison of different serological tests for brucellosis detection in small ruminants. Vet World. 2015;8(5):561-6doi: 10.14202/vetworld.2015.561-566.
Sadhu, D. B., Panchasara, H. H., Chauhan, H. C., Sutariya, D. R., Parmar, V. L., & Prajapati, H. B. (2015). Seroprevalence and comparison of different serological tests for brucellosis detection in small ruminants. Veterinary world, 8(5), 561-6. https://doi.org/10.14202/vetworld.2015.561-566
Sadhu, Dashrath B, et al. "Seroprevalence and comparison of different serological tests for brucellosis detection in small ruminants." Veterinary world vol. 8,5 (2015): 561-6. doi: https://doi.org/10.14202/vetworld.2015.561-566
Sadhu DB, Panchasara HH, Chauhan HC, Sutariya DR, Parmar VL, Prajapati HB. Seroprevalence and comparison of different serological tests for brucellosis detection in small ruminants. Vet World. 2015 May;8(5):561-6. doi: 10.14202/vetworld.2015.561-566. Epub 2015 May 04. PMID: 27047135; PMCID: PMC4774713.
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Vet World. 2015 May;8(5):561-6. doi: 10.14202/vetworld.2015.561-566. Epub 2015 May 04.

Seroprevalence and comparison of different serological tests for brucellosis detection in small ruminants.

Veterinary world

Dashrath B Sadhu, H H Panchasara, H C Chauhan, D R Sutariya, V L Parmar, H B Prajapati

Affiliations

  1. Department of Veterinary Medicine, Livestock Research Station, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Dantiwada, Gujarat, India.
  2. Department of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Dantiwada, Gujarat, India.

PMID: 27047135 PMCID: PMC4774713 DOI: 10.14202/vetworld.2015.561-566

Abstract

AIM: The aim was to study the seroprevalence and efficacy of the different serological tests used for detection of antibody against Brucella species in small ruminants of Banaskantha district of North-Gujarat.

MATERIALS AND METHODS: Total 1000 serum samples comprising of 485 from sheep and 515 from goat tested for detection of antibodies against the Brucella species by three different serological tests viz., Rose bengal plate test (RBPT), Standard tube agglutination test (STAT), and Indirect Enzyme-linked immunosorbent assay (I-ELISA).

RESULTS: The seroprevalence of brucellosis in small ruminants was 11.30%, 11.10%, and 8.80% by RBPT, STAT, and I-ELISA, respectively. The seroprevalence of brucellosis was found to be higher in sheep than goats. The sensitivity of RBPT was found slight more than STAT, but the specificity of both tests was same. In this study, the overall agreement of RBPT and STAT with I-ELISA was found 92.50% and 92.30% in small ruminants, respectively.

CONCLUSION: I-ELISA was a better serological test as compared to RBPT and STAT in the sense of sensitivity, specificity, and rapidity and it could be advocated for screening of brucellosis in sheep and goats.

Keywords: brucellosis; serological test; seroprevalence; small ruminant

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