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Gupta S, Gupta AK, Verma M, et al. A study to compare the plasma glucose levels obtained in sodium fluoride and citrate buffer tubes at a tertiary care hospital in Punjab. Int J Appl Basic Med Res. 2016;6(1):50-3doi: 10.4103/2229-516X.174010.
Gupta, S., Gupta, A. K., Verma, M., Singh, K., Kaur, A., Chopra, B., & Kaur, V. (2016). A study to compare the plasma glucose levels obtained in sodium fluoride and citrate buffer tubes at a tertiary care hospital in Punjab. International journal of applied & basic medical research, 6(1), 50-3. https://doi.org/10.4103/2229-516X.174010
Gupta, Shalini, et al. "A study to compare the plasma glucose levels obtained in sodium fluoride and citrate buffer tubes at a tertiary care hospital in Punjab." International journal of applied & basic medical research vol. 6,1 (2016): 50-3. doi: https://doi.org/10.4103/2229-516X.174010
Gupta S, Gupta AK, Verma M, Singh K, Kaur A, Chopra B, Kaur V. A study to compare the plasma glucose levels obtained in sodium fluoride and citrate buffer tubes at a tertiary care hospital in Punjab. Int J Appl Basic Med Res. 2016 Jan-Mar;6(1):50-3. doi: 10.4103/2229-516X.174010. PMID: 26958523; PMCID: PMC4765275.
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Int J Appl Basic Med Res. 2016 Jan-Mar;6(1):50-3. doi: 10.4103/2229-516X.174010.

A study to compare the plasma glucose levels obtained in sodium fluoride and citrate buffer tubes at a tertiary care hospital in Punjab.

International journal of applied & basic medical research

Shalini Gupta, Ashwani K Gupta, Minni Verma, Kamaljit Singh, Amandeep Kaur, Brinder Chopra, Vaneet Kaur

Affiliations

  1. Department of Biochemistry, Gian Sagar Medical College and Hospital, Ram Nagar, Patiala District, Punjab, India.
  2. Department of Pharmacology, Gian Sagar Medical College and Hospital, Ram Nagar, Patiala District, Punjab, India.

PMID: 26958523 PMCID: PMC4765275 DOI: 10.4103/2229-516X.174010

Abstract

OBJECTIVES: Recent guidelines for estimation of glucose recommend the use of citrate buffer tubes to inhibit glycolysis if the sample cannot be cooled immediately and separated within 30 min. These tubes are currently not available in India. We prepared the citrate tubes and compared the glucose results obtained with sodium fluoride tubes.

METHODS: Random blood samples of 44 apparently healthy volunteers were collected in three pairs of citrate buffer and sodium fluoride tubes during September to October 2013. They were labeled as 0 h, 1 h and 2 h samples indicating a delay in centrifugation to separate plasma. Glucose was analyzed on the fully auto analyzer in duplicates using glucose oxidase-peroxidase method.

RESULTS: The mean glucose concentrations at 0 h in citrate tubes were 105.8 ± 19.5 mg/dl compared to 99.6 ± 18.3 mg/dl in sodium fluoride tube. There was statistically significant difference in the glucose levels measured in plasma separated from citrate buffer tube and sodium fluoride tube at 0 h, 1 h, and 2 h. The difference between citrate and sodium fluoride tube results ranged from 6.1 mg/dl at 0 h to 7.4 mg/dl at 2 h. Glucose levels decreased significantly at 2 h in both citrate and sodium fluoride tubes.

CONCLUSION: There is a significant decrease in glucose levels in sodium fluoride tubes even with immediate separation of plasma. There is urgent need to standardize the preanalytical conditions for glucose estimation so that effective inhibition of glycolysis can be done.

Keywords: Citrate buffer; glucose estimation; glycolysis inhibition; sodium fluoride

References

  1. Clin Chem. 1989 Feb;35(2):315-7 - PubMed
  2. Ann Clin Biochem. 2013 Jan;50(Pt 1):3-5 - PubMed
  3. J Clin Pathol. 2007 Jul;60(7):820-3 - PubMed
  4. Clin Chem. 2002 Mar;48(3):436-72 - PubMed
  5. Clin Chem. 2011 Jun;57(6):793-8 - PubMed
  6. J Environ Public Health. 2013;2013:256151 - PubMed
  7. Clin Chem Lab Med. 2013 Oct;51(10):1943-9 - PubMed
  8. Clin Chem. 2009 May;55(5):1019-21 - PubMed
  9. Clin Chim Acta. 1988 Feb 29;172(1):101-8 - PubMed
  10. N Engl J Med. 2005 Oct 6;353(14):1454-62 - PubMed

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