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Varani J. Human skin organ culture for assessment of chemically induced skin damage. Expert Rev Dermatol. 2012;7(3):295-303doi: 10.1586/edm.12.24.
Varani, J. (2012). Human skin organ culture for assessment of chemically induced skin damage. Expert review of dermatology, 7(3), 295-303. https://doi.org/10.1586/edm.12.24
Varani, James. "Human skin organ culture for assessment of chemically induced skin damage." Expert review of dermatology vol. 7,3 (2012): 295-303. doi: https://doi.org/10.1586/edm.12.24
Varani J. Human skin organ culture for assessment of chemically induced skin damage. Expert Rev Dermatol. 2012 Jun 01;7(3):295-303. doi: 10.1586/edm.12.24. PMID: 26989431; PMCID: PMC4792533.
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Expert Rev Dermatol. 2012 Jun 01;7(3):295-303. doi: 10.1586/edm.12.24.

Human skin organ culture for assessment of chemically induced skin damage.

Expert review of dermatology

James Varani

Affiliations

  1. Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA, Tel.: +1 734 615 0298, [email protected].

PMID: 26989431 PMCID: PMC4792533 DOI: 10.1586/edm.12.24

Abstract

The move away from animal models for skin safety testing is inevitable. It is a question of when, not if. As skin safety studies move away from traditional animal-based approaches, a number of replacement technologies are becoming available. Human skin in organ culture is one such technology. Organ-cultured skin has several features that distinguish it from other technologies. First and foremost, organ-cultured skin is real skin. Almost by definition, therefore, it approximates the intact skin better than other alternative models. Organ culture is an easy-to-use and relatively inexpensive approach to preclinical safety assessment. Although organ culture is not likely to replace high-throughput enzyme assays or monolayer culture/skin equivalent cultures for initial compound assessment, organ culture should find use when the list of compounds to be evaluated is small and when simpler models have narrowed the dose range. Organ-cultured skin also provides a platform for mechanistic studies.

Keywords: contact irritation; contact sensitization; corrosivity; organ culture; organotypic culture; skin

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