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Araujo LC, de Souza IL, Vasconcelos LH, et al. Acute Aerobic Swimming Exercise Induces Distinct Effects in the Contractile Reactivity of Rat Ileum to KCl and Carbachol. Front Physiol. 2016;7:103doi: 10.3389/fphys.2016.00103.
Araujo, L. C., de Souza, I. L., Vasconcelos, L. H., Brito, A. d. e. . F., Queiroga, F. R., Silva, A. S., da Silva, P. M., Cavalcante, F. d. e. . A., & da Silva, B. A. (2016). Acute Aerobic Swimming Exercise Induces Distinct Effects in the Contractile Reactivity of Rat Ileum to KCl and Carbachol. Frontiers in physiology, 7103. https://doi.org/10.3389/fphys.2016.00103
Araujo, Layanne C da Cunha, et al. "Acute Aerobic Swimming Exercise Induces Distinct Effects in the Contractile Reactivity of Rat Ileum to KCl and Carbachol." Frontiers in physiology vol. 7 (2016): 103. doi: https://doi.org/10.3389/fphys.2016.00103
Araujo LC, de Souza IL, Vasconcelos LH, Brito Ade F, Queiroga FR, Silva AS, da Silva PM, Cavalcante Fde A, da Silva BA. Acute Aerobic Swimming Exercise Induces Distinct Effects in the Contractile Reactivity of Rat Ileum to KCl and Carbachol. Front Physiol. 2016 Mar 18;7:103. doi: 10.3389/fphys.2016.00103. eCollection 2016. PMID: 27047389; PMCID: PMC4796008.
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Front Physiol. 2016 Mar 18;7:103. doi: 10.3389/fphys.2016.00103. eCollection 2016.

Acute Aerobic Swimming Exercise Induces Distinct Effects in the Contractile Reactivity of Rat Ileum to KCl and Carbachol.

Frontiers in physiology

Layanne C da Cunha Araujo, Iara L L de Souza, Luiz H C Vasconcelos, Aline de Freitas Brito, Fernando R Queiroga, Alexandre S Silva, Patrícia M da Silva, Fabiana de Andrade Cavalcante, Bagnólia A da Silva

Affiliations

  1. Programa de Pós-graduação em Biologia Celular e Molecular, Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba João Pessoa, Brazil.
  2. Programa de Pós-graduação em Produtos Naturais e Sintéticos Bioativos, Centro de Ciências da Saúde, Universidade Federal da Paraíba João Pessoa, Brazil.
  3. Departamento de Educação Física, Centro de Ciências da Saúde, Universidade Federal da Paraíba João Pessoa, Brazil.
  4. Programa de Pós-graduação em Biologia Celular e Molecular, Centro de Ciências Exatas e da Natureza, Universidade Federal da ParaíbaJoão Pessoa, Brazil; Departamento de Biologia Molecular, Centro de Ciências Exatas e da Natureza, Universidade Federal da ParaíbaJoão Pessoa, Brazil.
  5. Departamento de Fisiologia e Patologia, Centro de Ciência da Saúde, Universidade Federal da Paraíba João Pessoa, Brazil.
  6. Programa de Pós-graduação em Biologia Celular e Molecular, Centro de Ciências Exatas e da Natureza, Universidade Federal da ParaíbaJoão Pessoa, Brazil; Programa de Pós-graduação em Produtos Naturais e Sintéticos Bioativos, Centro de Ciências da Saúde, Universidade Federal da ParaíbaJoão Pessoa, Brazil; Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal da ParaíbaJoão Pessoa, Brazil.

PMID: 27047389 PMCID: PMC4796008 DOI: 10.3389/fphys.2016.00103

Abstract

Aerobic exercise promotes short-term physiological changes in the intestinal smooth muscle associated to the ischemia-reperfusion process; however, few studies have demonstrated its effect on the intestinal contractile function. Thus, this work describes our observations regarding the influence of acute aerobic swimming exercise in the contractile reactivity, oxidative stress, and morphology of rat ileum. Wistar rats were divided into sedentary (SED) and acutely exercised (EX-AC) groups. Animals were acclimated by 10, 10, and 30 min of swimming exercise in intercalated days 1 week before exercise. Then they were submitted to forced swimming for 1 h with a metal of 3% of their body weight attached to their body. Animals were euthanized immediately after the exercise section and the ileum was suspended in organ baths for monitoring isotonic contractions. The analysis of lipid peroxidation was performed in order to determinate the malondialdehyde (MDA) levels as a marker of oxidative stress, and intestinal smooth muscle morphology by histological staining. Cumulative concentration-response curves to KCl were altered in the EX-AC with an increase in both its efficacy and potency (E max = 153.2 ± 2.8%, EC 50 = 1.3 ± 0.1 × 10(-2) M) compared to the SED group (E max = 100%, EC 50 = 1.8 ± 0.1 × 10(-2) M). Interestingly, carbachol had its efficacy and potency reduced in the EX-AC (E max = 67.1 ± 1.4%, EC 50 = 9.8 ± 1.4 × 10(-7) M) compared to the SED group (E max = 100%, EC 50 = 2.0 ± 0.2 × 10(-7) M). The exercise did not alter the MDA levels in the ileum (5.4 ± 0.6 μ mol/mL) in the EX-AC compared to the SED group (8.4 ± 1.7 μ mol/mL). Moreover, neither the circular nor the longitudinal smooth muscle layers thickness were modified by the exercise (66.2 ± 6.0 and 40.2 ± 2.6 μm, respectively), compared to the SED group (61.6 ± 6.4 and 34.8 ± 3.7 μm, respectively). Therefore, the ileum sensitivity to contractile agents is differentially altered by the acute aerobic swimming exercise, without affecting the oxidative stress and the morphology of ileum smooth muscle.

Keywords: acute exercise; contractile reactivity; gastrointestinal tract; rat ileum; swimming

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