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Ozyigit II, Filiz E, Vatansever R, et al. Identification and Comparative Analysis of H2O2-Scavenging Enzymes (Ascorbate Peroxidase and Glutathione Peroxidase) in Selected Plants Employing Bioinformatics Approaches. Front Plant Sci. 2016;7:301doi: 10.3389/fpls.2016.00301.
Ozyigit, I. I., Filiz, E., Vatansever, R., Kurtoglu, K. Y., Koc, I., Öztürk, M. X., & Anjum, N. A. (2016). Identification and Comparative Analysis of H2O2-Scavenging Enzymes (Ascorbate Peroxidase and Glutathione Peroxidase) in Selected Plants Employing Bioinformatics Approaches. Frontiers in plant science, 7301. https://doi.org/10.3389/fpls.2016.00301
Ozyigit, Ibrahim I, et al. "Identification and Comparative Analysis of H2O2-Scavenging Enzymes (Ascorbate Peroxidase and Glutathione Peroxidase) in Selected Plants Employing Bioinformatics Approaches." Frontiers in plant science vol. 7 (2016): 301. doi: https://doi.org/10.3389/fpls.2016.00301
Ozyigit II, Filiz E, Vatansever R, Kurtoglu KY, Koc I, Öztürk MX, Anjum NA. Identification and Comparative Analysis of H2O2-Scavenging Enzymes (Ascorbate Peroxidase and Glutathione Peroxidase) in Selected Plants Employing Bioinformatics Approaches. Front Plant Sci. 2016 Mar 22;7:301. doi: 10.3389/fpls.2016.00301. eCollection 2016. PMID: 27047498; PMCID: PMC4802093.
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Front Plant Sci. 2016 Mar 22;7:301. doi: 10.3389/fpls.2016.00301. eCollection 2016.

Identification and Comparative Analysis of H2O2-Scavenging Enzymes (Ascorbate Peroxidase and Glutathione Peroxidase) in Selected Plants Employing Bioinformatics Approaches.

Frontiers in plant science

Ibrahim I Ozyigit, Ertugrul Filiz, Recep Vatansever, Kuaybe Y Kurtoglu, Ibrahim Koc, Münir X Öztürk, Naser A Anjum

Affiliations

  1. Department of Biology, Faculty of Science and Arts, Marmara University Istanbul, Turkey.
  2. Department of Crop and Animal Production, Cilimli Vocational School, Düzce University Düzce, Turkey.
  3. Department of Biology, Faculty of Science and Arts, Marmara UniversityIstanbul, Turkey; Department of Molecular Biology and Genetics, Faculty of Science, Istanbul Medeniyet UniversityIstanbul, Turkey.
  4. Department of Molecular Biology and Genetics, Faculty of Science, Gebze Technical University Kocaeli, Turkey.
  5. Botany Department/Center for Environmental Studies, Ege UniversityIzmir, Turkey; Faculty of Forestry, Universiti Putra MalaysiaSelangor, Malaysia.
  6. Centre for Environmental and Marine Studies and Department of Chemistry, University of Aveiro Aveiro, Portugal.

PMID: 27047498 PMCID: PMC4802093 DOI: 10.3389/fpls.2016.00301

Abstract

Among major reactive oxygen species (ROS), hydrogen peroxide (H2O2) exhibits dual roles in plant metabolism. Low levels of H2O2 modulate many biological/physiological processes in plants; whereas, its high level can cause damage to cell structures, having severe consequences. Thus, steady-state level of cellular H2O2 must be tightly regulated. Glutathione peroxidases (GPX) and ascorbate peroxidase (APX) are two major ROS-scavenging enzymes which catalyze the reduction of H2O2 in order to prevent potential H2O2-derived cellular damage. Employing bioinformatics approaches, this study presents a comparative evaluation of both GPX and APX in 18 different plant species, and provides valuable insights into the nature and complex regulation of these enzymes. Herein, (a) potential GPX and APX genes/proteins from 18 different plant species were identified, (b) their exon/intron organization were analyzed, (c) detailed information about their physicochemical properties were provided, (d) conserved motif signatures of GPX and APX were identified, (e) their phylogenetic trees and 3D models were constructed, (f) protein-protein interaction networks were generated, and finally (g) GPX and APX gene expression profiles were analyzed. Study outcomes enlightened GPX and APX as major H2O2-scavenging enzymes at their structural and functional levels, which could be used in future studies in the current direction.

Keywords: ROS; antioxidant; chloroplast; mitochondria; peroxisome; signal transduction

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