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Sun Z, Lawson DA, Sinclair E, et al. Endovascular biopsy: Strategy for analyzing gene expression profiles of individual endothelial cells obtained from human vessels. Biotechnol Rep (Amst). 2015;7:157-165doi: 10.1016/j.btre.2015.07.001.
Sun, Z., Lawson, D. A., Sinclair, E., Wang, C. Y., Lai, M. D., Hetts, S. W., Higashida, R. T., Dowd, C. F., Halbach, V. V., Werb, Z., Su, H., & Cooke, D. L. (2015). Endovascular biopsy: Strategy for analyzing gene expression profiles of individual endothelial cells obtained from human vessels. Biotechnology reports (Amsterdam, Netherlands), 7157-165. https://doi.org/10.1016/j.btre.2015.07.001
Sun, Zhengda, et al. "Endovascular biopsy: Strategy for analyzing gene expression profiles of individual endothelial cells obtained from human vessels." Biotechnology reports (Amsterdam, Netherlands) vol. 7 (2015): 157-165. doi: https://doi.org/10.1016/j.btre.2015.07.001
Sun Z, Lawson DA, Sinclair E, Wang CY, Lai MD, Hetts SW, Higashida RT, Dowd CF, Halbach VV, Werb Z, Su H, Cooke DL. Endovascular biopsy: Strategy for analyzing gene expression profiles of individual endothelial cells obtained from human vessels. Biotechnol Rep (Amst). 2015 Sep;7:157-165. doi: 10.1016/j.btre.2015.07.001. Epub 2015 Aug 01. PMID: 26989654; PMCID: PMC4792280.
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Biotechnol Rep (Amst). 2015 Sep;7:157-165. doi: 10.1016/j.btre.2015.07.001. Epub 2015 Aug 01.

Endovascular biopsy: Strategy for analyzing gene expression profiles of individual endothelial cells obtained from human vessels.

Biotechnology reports (Amsterdam, Netherlands)

Zhengda Sun, Devon A Lawson, Elizabeth Sinclair, Chih-Yang Wang, Ming-Derg Lai, Steven W Hetts, Randall T Higashida, Christopher F Dowd, Van V Halbach, Zena Werb, Hua Su, Daniel L Cooke

Affiliations

  1. Division of Neurointerventional Radiology, Department of Radiology and Biomedical Imaging, University of California, San Francisco, CA, USA.
  2. Department of Anatomy, University of California, San Francisco, CA, USA.
  3. Division of Experimental Medicine, Department of Medicine, University of California, San Francisco, CA, USA.
  4. Department of Anatomy, University of California, San Francisco, CA, USA; Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
  5. Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
  6. Center for Cerebrovascular Research, Department of Anesthesia and Perioperative Care, University of California, San Francisco, CA, USA.

PMID: 26989654 PMCID: PMC4792280 DOI: 10.1016/j.btre.2015.07.001

Abstract

PURPOSE: To develop a strategy of achieving targeted collection of endothelial cells (ECs) by endovascular methods and analyzing the gene expression profiles of collected single ECs.

METHODS AND RESULTS: 134 ECs and 37 leukocytes were collected from four patients' intra-iliac artery endovascular guide wires by fluorescence activated cell sorting (FACS) and analyzed by single-cell quantitative RT-PCR for expression profile of 48 genes. Compared to CD45

CONCLUSION: Combination of endovascular device sampling, FACS and single-cell quantitative RT-PCR is a feasible method for analyzing EC gene expression profile in vascular lesions.

Keywords: Gene expression of artery endothelial cells; Single cell quantitative RT-PCR; Targeted endothelial cell sampling

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