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Li Z, Wang J, Zhou T, et al. Establishment of a colorectal cancer nude mouse visualization model of HIF-1α overexpression. Oncol Lett. 2016;11(4):2725-2732doi: 10.3892/ol.2016.4287.
Li, Z., Wang, J., Zhou, T., & Ye, X. (2016). Establishment of a colorectal cancer nude mouse visualization model of HIF-1α overexpression. Oncology letters, 11(4), 2725-2732. https://doi.org/10.3892/ol.2016.4287
Li, Zhe, et al. "Establishment of a colorectal cancer nude mouse visualization model of HIF-1α overexpression." Oncology letters vol. 11,4 (2016): 2725-2732. doi: https://doi.org/10.3892/ol.2016.4287
Li Z, Wang J, Zhou T, Ye X. Establishment of a colorectal cancer nude mouse visualization model of HIF-1α overexpression. Oncol Lett. 2016 Apr;11(4):2725-2732. doi: 10.3892/ol.2016.4287. Epub 2016 Mar 01. PMID: 27073543; PMCID: PMC4812287.
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Oncol Lett. 2016 Apr;11(4):2725-2732. doi: 10.3892/ol.2016.4287. Epub 2016 Mar 01.

Establishment of a colorectal cancer nude mouse visualization model of HIF-1α overexpression.

Oncology letters

Zhe Li, Jiye Wang, Tianbao Zhou, Xiaolei Ye

Affiliations

  1. Department of Thyroid and Breast Surgery, The Shanghai Tenth People's Hospital, Tong Ji University, Shanghai 200072, P.R. China.
  2. Department of Criminal Science and Technology, Zhejiang Police College, Hangzhou, Zhejiang 310053, P.R. China.
  3. Hepatobiliary Surgery Centre, The Ningbo No. 2 Hospital, Ningbo, Zhejiang 315010, P.R. China.
  4. Division of Drugs and Pharamcology, Ningbo Institute of Medical Sciences, Ningbo, Zhejiang 315020, P.R. China.

PMID: 27073543 PMCID: PMC4812287 DOI: 10.3892/ol.2016.4287

Abstract

The aim of the present study was to establish a model of tumor cell growth and visualize HIF-1α overexpression in a nude mouse xenograft model of colorectal cancer (CRC). In the study, HIF-1α lentiviral vector and helper plasmid were co-transfected into 293T packaging cells using a liposome method, and the virus was collected following transfection and used to infect CRC SW480, SW620, LoVo and HCT116 cells. Puromycin was used for the selection and large-scale amplification of the stable HIF-1α expression of green fluorescent protein (GFP)-positive cells. HIF-1α-expressing cells were injected intraperitoneally into a nude mouse xenograft model, and resulting tumor nodules was separated and confirmed using an inverted fluorescence microscope. The results demonstrated that HIF-1α was not expressed in CRC cells in normoxic conditions. When treated with CoCl

Keywords: HIF-1α; colorectal cancer; green fluorescent protein; lenti virus; stable transfection; xenograft model

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