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Peers MK, Toogood HS, Heyes DJ, et al. Light-driven biocatalytic reduction of α,β-unsaturated compounds by ene reductases employing transition metal complexes as photosensitizers. Catal Sci Technol. 2015;6(1):169-177doi: 10.1039/c5cy01642h.
Peers, M. K., Toogood, H. S., Heyes, D. J., Mansell, D., Coe, B. J., & Scrutton, N. S. (2016). Light-driven biocatalytic reduction of α,β-unsaturated compounds by ene reductases employing transition metal complexes as photosensitizers. Catalysis science & technology, 6(1), 169-177. https://doi.org/10.1039/c5cy01642h
Peers, Martyn K, et al. "Light-driven biocatalytic reduction of α,β-unsaturated compounds by ene reductases employing transition metal complexes as photosensitizers." Catalysis science & technology vol. 6,1 (2016): 169-177. doi: https://doi.org/10.1039/c5cy01642h
Peers MK, Toogood HS, Heyes DJ, Mansell D, Coe BJ, Scrutton NS. Light-driven biocatalytic reduction of α,β-unsaturated compounds by ene reductases employing transition metal complexes as photosensitizers. Catal Sci Technol. 2016 Jan 07;6(1):169-177. doi: 10.1039/c5cy01642h. Epub 2015 Oct 26. PMID: 27019691; PMCID: PMC4786955.
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Catal Sci Technol. 2016 Jan 07;6(1):169-177. doi: 10.1039/c5cy01642h. Epub 2015 Oct 26.

Light-driven biocatalytic reduction of α,β-unsaturated compounds by ene reductases employing transition metal complexes as photosensitizers.

Catalysis science & technology

Martyn K Peers, Helen S Toogood, Derren J Heyes, David Mansell, Benjamin J Coe, Nigel S Scrutton

Affiliations

  1. Manchester Institute of Biotechnology , Faculty of Life Sciences , University of Manchester , 131 Princess Street , Manchester , M1 7DN , UK . Email: [email protected].
  2. School of Chemistry , University of Manchester , Oxford Road , Manchester , M13 9PL , UK.

PMID: 27019691 PMCID: PMC4786955 DOI: 10.1039/c5cy01642h

Abstract

Efficient and cost effective nicotinamide cofactor regeneration is essential for industrial-scale bio-hydrogenations employing flavin-containing biocatalysts such as the Old Yellow Enzymes. A direct flavin regeneration system using visible light to initiate a photoredox cycle and drive biocatalysis is described, and shown to be effective in driving biocatalytic activated alkene reduction. Using Ru(ii) or Ir(iii) complexes as photosensitizers, coupled with an electron transfer mediator (methyl viologen) and sacrificial electron donor (triethanolamine) drives catalytic turnover of two Old Yellow Enzymes with multiple oxidative substrates. Therefore, there is great potential in the development of light-driven biocatalytic systems, providing an alternative to the reliance on enzyme-based cofactor regeneration systems.

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