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Conesa JJ, Otón J, Chiappi M, et al. Intracellular nanoparticles mass quantification by near-edge absorption soft X-ray nanotomography. Sci Rep. 2016;6:22354doi: 10.1038/srep22354.
Conesa, J. J., Otón, J., Chiappi, M., Carazo, J. M., Pereiro, E., Chichón, F. J., & Carrascosa, J. L. (2016). Intracellular nanoparticles mass quantification by near-edge absorption soft X-ray nanotomography. Scientific reports, 622354. https://doi.org/10.1038/srep22354
Conesa, Jose Javier, et al. "Intracellular nanoparticles mass quantification by near-edge absorption soft X-ray nanotomography." Scientific reports vol. 6 (2016): 22354. doi: https://doi.org/10.1038/srep22354
Conesa JJ, Otón J, Chiappi M, Carazo JM, Pereiro E, Chichón FJ, Carrascosa JL. Intracellular nanoparticles mass quantification by near-edge absorption soft X-ray nanotomography. Sci Rep. 2016 Mar 10;6:22354. doi: 10.1038/srep22354. PMID: 26960695; PMCID: PMC4785355.
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Sci Rep. 2016 Mar 10;6:22354. doi: 10.1038/srep22354.

Intracellular nanoparticles mass quantification by near-edge absorption soft X-ray nanotomography.

Scientific reports

Jose Javier Conesa, Joaquín Otón, Michele Chiappi, Jose María Carazo, Eva Pereiro, Francisco Javier Chichón, José L Carrascosa

Affiliations

  1. Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, 28049 Madrid, Spain.
  2. ALBA Synchrotron Light Source, MISTRAL Beamline - Experiments Division, 08290 Cerdanyola del Vallès, Barcelona, Spain.
  3. Unidad Asociada CNB-Instituto Madrileño de Estudios Avanzados en Nanociencia (IMDEA Nanociencia), Cantoblanco, 28049 Madrid, Spain.

PMID: 26960695 PMCID: PMC4785355 DOI: 10.1038/srep22354

Abstract

We used soft X-ray three-dimensional imaging to quantify the mass of superparamagnetic iron oxide nanoparticles (SPION) within whole cells, by exploiting the iron oxide differential absorption contrast. Near-edge absorption soft X-ray nanotomography (NEASXT) combines whole-cell 3D structure determination at 50 nm resolution, with 3D elemental mapping and high throughput. We detected three-dimensional distribution of SPIONs within cells with 0.3 g/cm(3) sensitivity, sufficient for detecting the density corresponding to a single nanoparticle.

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