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Bicalho B, Serrano K, Dos Santos Pereira A, et al. Blood Bag Plasticizers Influence Red Blood Cell Vesiculation Rate without Altering the Lipid Composition of the Vesicles. Transfus Med Hemother. 2015;43(1):19-26doi: 10.1159/000441639.
Bicalho, B., Serrano, K., Dos Santos Pereira, A., Devine, D. V., & Acker, J. P. (2016). Blood Bag Plasticizers Influence Red Blood Cell Vesiculation Rate without Altering the Lipid Composition of the Vesicles. Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie, 43(1), 19-26. https://doi.org/10.1159/000441639
Bicalho, Beatriz, et al. "Blood Bag Plasticizers Influence Red Blood Cell Vesiculation Rate without Altering the Lipid Composition of the Vesicles." Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie vol. 43,1 (2016): 19-26. doi: https://doi.org/10.1159/000441639
Bicalho B, Serrano K, Dos Santos Pereira A, Devine DV, Acker JP. Blood Bag Plasticizers Influence Red Blood Cell Vesiculation Rate without Altering the Lipid Composition of the Vesicles. Transfus Med Hemother. 2016 Jan;43(1):19-26. doi: 10.1159/000441639. Epub 2015 Oct 29. PMID: 27022319; PMCID: PMC4797466.
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Transfus Med Hemother. 2016 Jan;43(1):19-26. doi: 10.1159/000441639. Epub 2015 Oct 29.

Blood Bag Plasticizers Influence Red Blood Cell Vesiculation Rate without Altering the Lipid Composition of the Vesicles.

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie

Beatriz Bicalho, Katherine Serrano, Alberto Dos Santos Pereira, Dana V Devine, Jason P Acker

Affiliations

  1. Canadian Blood Services, Center for Innovation, Edmonton, AB, Canada.
  2. Canadian Blood Services, Center for Innovation, Vancouver, BC, Canada; Department of Pathology and Laboratory Medicine at the Center for Blood Research, University of British Columbia, Vancouver, BC, Canada.
  3. Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada.
  4. Canadian Blood Services, Center for Innovation, Edmonton, AB, Canada; Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada.

PMID: 27022319 PMCID: PMC4797466 DOI: 10.1159/000441639

Abstract

BACKGROUND: Polyvinyl chloride (PVC) plasticized with di(2-ethylhexyl) phthalate (DEHP) is commonly used for blood collection and storage. DEHP has protective effects on RBC membranes, but is also a toxin.

METHODS: A paired study was conducted to investigate the influence of DEHP and two alternative plasticizers, 1,2-cyclohexane-dicarboxylic acid diisononyl ester (DINCH) and n-butyryl-tri-n-hexyl citrate (BTHC), on the preservation of RBCs stored for 42 days in PVC pediatric bags. The RBC membrane was evaluated for supernatant hemoglobin (Hb), release of extracellular microvesicles (EVs), osmotic fragility, deformability, and lipid composition.

RESULTS: In BTHC-plasticized bags, the supernatant Hb increase during storage was 2 times greater than in DINCH- and DEHP-plasticized bags. By day 21, EV concentrations had doubled from day-5 levels in DINCH- and DEHP-, and trebled in BTHC-plasticized bags. RBC mean cell volumes were greater in BTHC- than in DINCH- or DEHP-plasticized bags (p < 0.001). Osmotic fragility differed significantly among plasticizers (p < 0.01). After day 21, RBC deformability decreased in all, but to a greater extent in the bags with BTHC. Phospholipid composition of RBCs and EVs was not different among plasticizers.

CONCLUSION: Membrane stabilization capacity differed among the plasticizers. RBC in BTHC bags stored more poorly, while DEHP and DINCH bags offered better protection against vesiculation, osmotic stress, and Hb loss.

Keywords: BTHC; DEHP; DINCH; Membrane; Red blood cell

References

  1. J Perinatol. 2011 Aug;31(8):551-60 - PubMed
  2. Biochim Biophys Acta. 2013 Feb;1828(2):317-26 - PubMed
  3. J Biomed Mater Res B Appl Biomater. 2014 May;102(4):721-8 - PubMed
  4. Oxid Med Cell Longev. 2009 Jul-Aug;2(3):166-71 - PubMed
  5. Transfusion. 2012 Jul;52(7):1439-45 - PubMed
  6. Vox Sang. 1991;61(1):8-13 - PubMed
  7. Blood. 1987 Jul;70(1):319-23 - PubMed
  8. Vox Sang. 1978;34(2):81-6 - PubMed
  9. Transfusion. 2002 Jul;42(7):830-5 - PubMed
  10. Curr Opin Pediatr. 2013 Apr;25(2):247-54 - PubMed
  11. J Mater Sci Mater Med. 2013 Aug;24(8):1985-92 - PubMed
  12. Toxicol Lett. 2009 Jul 24;188(2):157-60 - PubMed
  13. Clin Hemorheol Microcirc. 2011;47(3):229-39 - PubMed
  14. Int J Hyg Environ Health. 2007 Oct;210(5):623-34 - PubMed
  15. Vox Sang. 1985;48(3):150-5 - PubMed
  16. Blood. 1988 Feb;71(2):448-52 - PubMed
  17. Vox Sang. 2015 Oct;109(3):214-20 - PubMed
  18. Transfusion. 2013 Oct;53(10):2258-67 - PubMed
  19. Vox Sang. 2010 Feb;98(2):116-23 - PubMed
  20. Vox Sang. 2014 Jan;106(1):1-13 - PubMed
  21. Transfusion. 1991 Jan;31(1):26-9 - PubMed
  22. Biochem Soc Trans. 2013 Feb 1;41(1):288-92 - PubMed
  23. Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7566-71 - PubMed
  24. Transfusion. 2014 Oct;54(10):2534-43 - PubMed
  25. Blood. 1984 Dec;64(6):1270-6 - PubMed
  26. Blood. 2014 Jan 30;123(5):687-96 - PubMed

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