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Kwon J, Kim NH, Choi I. ROCK activity regulates functional tight junction assembly during blastocyst formation in porcine parthenogenetic embryos. PeerJ. 2016;4:e1914doi: 10.7717/peerj.1914.
Kwon, J., Kim, N. H., & Choi, I. (2016). ROCK activity regulates functional tight junction assembly during blastocyst formation in porcine parthenogenetic embryos. PeerJ, 4e1914. https://doi.org/10.7717/peerj.1914
Kwon, Jeongwoo, et al. "ROCK activity regulates functional tight junction assembly during blastocyst formation in porcine parthenogenetic embryos." PeerJ vol. 4 (2016): e1914. doi: https://doi.org/10.7717/peerj.1914
Kwon J, Kim NH, Choi I. ROCK activity regulates functional tight junction assembly during blastocyst formation in porcine parthenogenetic embryos. PeerJ. 2016 Apr 11;4:e1914. doi: 10.7717/peerj.1914. eCollection 2016. PMID: 27077008; PMCID: PMC4830244.
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PeerJ. 2016 Apr 11;4:e1914. doi: 10.7717/peerj.1914. eCollection 2016.

ROCK activity regulates functional tight junction assembly during blastocyst formation in porcine parthenogenetic embryos.

PeerJ

Jeongwoo Kwon, Nam-Hyung Kim, Inchul Choi

Affiliations

  1. Department of Animal Science, Chungbuk National University , Cheongju Chungbuk , South Korea.
  2. Department of Animal and Dairy Sciences, Chungnam National University , Daejeon Chungnam , South Korea.

PMID: 27077008 PMCID: PMC4830244 DOI: 10.7717/peerj.1914

Abstract

The Rho-associated coiled-coil-containing protein serine/threonine kinases 1 and 2 (ROCK1 and ROCK2) are Rho subfamily GTPase downstream effectors that regulate cell migration, intercellular adhesion, cell polarity, and cell proliferation by stimulating actin cytoskeleton reorganization. Inhibition of ROCK proteins affects specification of the trophectoderm (TE) and inner cell mass (ICM) lineages, compaction, and blastocyst cavitation. However, the molecules involved in blastocyst formation are not known. Here, we examined developmental competence and levels of adherens/tight junction (AJ/TJ) constituent proteins, such as CXADR, OCLN, TJP1, and CDH1, as well as expression of their respective mRNAs, after treating porcine parthenogenetic four-cell embryos with Y-27632, a specific inhibitor of ROCK, at concentrations of 0, 10, 20, 100 µM for 24 h. Following this treatment, the blastocyst development rates were 39.1, 20.7, 10.0, and 0% respectively. In embryos treated with 20 µM treatment, expression levels of CXADR, OCLN, TJP1, and CDH1 mRNA and protein molecules were significantly reduced (P < 0.05). FITC-dextran uptake assay revealed that the treatment caused an increase in TE TJ permeability. Interestingly, the majority of the four-cell and morula embryos treated with 20 µM Y-27643 for 24 h showed defective compaction and cavitation. Taken together, our results indicate that ROCK activity may differentially affect assembly of AJ/TJs as well as regulate expression of genes encoding junctional proteins.

Keywords: Blastocyst; Porcine embryo; Preimplantation; Tight junction

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