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Ecker A, Araujo Vieira F, de Souza Prestes A, et al. Effect of Syzygium cumini and Bauhinia forficata aqueous-leaf extracts on oxidative and mitochondrial parameters in vitro. EXCLI J. 2015;14:1219-31doi: 10.17179/excli2015-576.
Ecker, A., Araujo Vieira, F., de Souza Prestes, A., Mulling Dos Santos, M., Ramos, A., Dias Ferreira, R., Teixeira de Macedo, G., Vargas Klimaczewski, C., Lopes Seeger, R., Teixeira da Rocha, J. B., & de Vargas Barbosa, N. B. (2015). Effect of Syzygium cumini and Bauhinia forficata aqueous-leaf extracts on oxidative and mitochondrial parameters in vitro. EXCLI journal, 141219-31. https://doi.org/10.17179/excli2015-576
Ecker, Assis, et al. "Effect of Syzygium cumini and Bauhinia forficata aqueous-leaf extracts on oxidative and mitochondrial parameters in vitro." EXCLI journal vol. 14 (2015): 1219-31. doi: https://doi.org/10.17179/excli2015-576
Ecker A, Araujo Vieira F, de Souza Prestes A, Mulling Dos Santos M, Ramos A, Dias Ferreira R, Teixeira de Macedo G, Vargas Klimaczewski C, Lopes Seeger R, Teixeira da Rocha JB, de Vargas Barbosa NB. Effect of Syzygium cumini and Bauhinia forficata aqueous-leaf extracts on oxidative and mitochondrial parameters in vitro. EXCLI J. 2015 Nov 27;14:1219-31. doi: 10.17179/excli2015-576. eCollection 2015. PMID: 27152111; PMCID: PMC4849105.
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EXCLI J. 2015 Nov 27;14:1219-31. doi: 10.17179/excli2015-576. eCollection 2015.

Effect of Syzygium cumini and Bauhinia forficata aqueous-leaf extracts on oxidative and mitochondrial parameters in vitro.

EXCLI journal

Assis Ecker, Francielli Araujo Vieira, Alessandro de Souza Prestes, Matheus Mulling Dos Santos, Angelica Ramos, Rafael Dias Ferreira, Gabriel Teixeira de Macedo, Claudia Vargas Klimaczewski, Rodrigo Lopes Seeger, João Batista Teixeira da Rocha, Nilda B de Vargas Barbosa

Affiliations

  1. Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil.

PMID: 27152111 PMCID: PMC4849105 DOI: 10.17179/excli2015-576

Abstract

Aqueous-leaf extract of Syzygium cumini and Bauhinia forficata are traditionally used in the treatment of diabetes and cancer, especially in South America, Africa, and Asia. In this study, we analyzed the effects of these extracts on oxidative and mitochondrial parameters in vitro, as well as their protective activities against toxic agents. Phytochemical screenings of the extracts were carried out by HPLC analysis. The in vitro antioxidant capacities were compared by DPPH radical scavenging and Fe(2+) chelating activities. Mitochondrial parameters observed were swelling, lipid peroxidation and dehydrogenase activity. The major chemical constituent of S. cumini was rutin. In B. forficata were predominant quercetin and gallic acid. S. cumini reduced DPPH radical more than B. forficata, and showed iron chelating activity at all tested concentrations, while B. forficata had not similar property. In mitochondria, high concentrations of B. forficata alone induced a decrease in mitochondrial dehydrogenase activity, but low concentrations of this extract prevented the effect induced by Fe(2+)+H2O2. This was also observed with high concentrations of S. cumini. Both extracts partially prevented the lipid peroxidation induced by Fe(2+)/citrate. S. cumini was effective against mitochondrial swelling induced by Ca(2+), while B. forficata alone induced swelling more than Ca(2+). This study suggests that leaf extract of S. cumini might represent a useful therapeutic for the treatment of diseases related with mitochondrial dysfunctions. On the other hand, the consumption of B. forficata should be avoided because mitochondrial damages were observed, and this possibly may pose risk to human health.

Keywords: Bauhinia forficate; Syzygium cumini; antioxidant properties; mitochondria

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