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Movahedi Najafabadi BA, Abnosi MH. Boron Induces Early Matrix Mineralization via Calcium Deposition and Elevation of Alkaline Phosphatase Activity in Differentiated Rat Bone Marrow Mesenchymal Stem Cells. Cell J. 2016;18(1):62-73doi: 10.22074/cellj.2016.3988.
Movahedi Najafabadi, B. A., & Abnosi, M. H. (2016). Boron Induces Early Matrix Mineralization via Calcium Deposition and Elevation of Alkaline Phosphatase Activity in Differentiated Rat Bone Marrow Mesenchymal Stem Cells. Cell journal, 18(1), 62-73. https://doi.org/10.22074/cellj.2016.3988
Movahedi Najafabadi, Bent-Al-Hoda, and Abnosi, Mohammad Hussein. "Boron Induces Early Matrix Mineralization via Calcium Deposition and Elevation of Alkaline Phosphatase Activity in Differentiated Rat Bone Marrow Mesenchymal Stem Cells." Cell journal vol. 18,1 (2016): 62-73. doi: https://doi.org/10.22074/cellj.2016.3988
Movahedi Najafabadi BA, Abnosi MH. Boron Induces Early Matrix Mineralization via Calcium Deposition and Elevation of Alkaline Phosphatase Activity in Differentiated Rat Bone Marrow Mesenchymal Stem Cells. Cell J. 2016;18(1):62-73. doi: 10.22074/cellj.2016.3988. Epub 2016 Apr 04. PMID: 27054120; PMCID: PMC4819387.
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Cell J. 2016;18(1):62-73. doi: 10.22074/cellj.2016.3988. Epub 2016 Apr 04.

Boron Induces Early Matrix Mineralization via Calcium Deposition and Elevation of Alkaline Phosphatase Activity in Differentiated Rat Bone Marrow Mesenchymal Stem Cells.

Cell journal

Bent-Al-Hoda Movahedi Najafabadi, Mohammad Hussein Abnosi

Affiliations

  1. Department of Biology, Faculty of Sciences, Arak University, Arak, Iran.

PMID: 27054120 PMCID: PMC4819387 DOI: 10.22074/cellj.2016.3988

Abstract

OBJECTIVE: Boron (B) is essential for plant development and might be an essential micronutrient for animals and humans. This study was conducted to characterize the impact of boric acid (BA) on the cellular and molecular nature of differentiated rat bone marrow mesenchymal stem cells (BMSCs).

MATERIALS AND METHODS: In this experimental study, BMSCs were extracted and expanded to the 3rdpassage, then cultured in Dulbecco's Modified Eagle's Medium (DMEM) complemented with osteogenic media as well as 6 ng/ml and 6 µg/ml of BA. After 5, 10, 15 and 21 days the viability and the level of mineralization was determined using MTT assay and alizarin red respectively. In addition, the morphology, nuclear diameter and cytoplasmic area of the cells were studied with the help of fluorescent dye. The concentration of calcium, activity of alanine transaminase (ALT), aspartate transaminase (AST), lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) as well as sodium and potassium levels were also evaluated using commercial kits and a flame photometer respectively.

RESULTS: Although 6 µg/ml of BA was found to be toxic, a concentration of 6 ng/ml increased the osteogenic ability of the cell significantly throughout the treatment. In addition it was observed that B treatment caused the early induction of matrix mineralization compared to controls.

CONCLUSION: Although more investigation is required, we suggest the prescription of a very low concentration of B in the form of BA or foods containing BA, in groups at high risk of osteoporosis or in the case of bone fracture.

Keywords: Boric Acid; Mesenchymal Stem Cells; Morphology; Osteoblasts

References

  1. Mol Cell Biochem. 2007 Oct;304(1-2):167-79 - PubMed
  2. Biofactors. 2004;20(3):161-71 - PubMed
  3. Cryobiology. 2014 Feb;68(1):139-46 - PubMed
  4. Biol Trace Elem Res. 2011 Dec;144(1-3):306-15 - PubMed
  5. Proteins. 1988;4(3):205-10 - PubMed
  6. Iran J Basic Med Sci. 2015 Apr;18(4):317-24 - PubMed
  7. Rev Environ Contam Toxicol. 2013;225:57-75 - PubMed
  8. J Trace Elem Med Biol. 2010 Oct;24(4):243-50 - PubMed
  9. Met Ions Life Sci. 2013;13:29-47 - PubMed
  10. Nutr Health. 1999;13(1):31-7 - PubMed
  11. J Trace Elem Med Biol. 2011 Jan;25(1):54-8 - PubMed
  12. J Cell Biol. 2007 Feb 26;176(5):709-18 - PubMed
  13. J Trace Elem Med Biol. 2002;16(4):239-44 - PubMed
  14. J Gen Physiol. 1988 Aug;92(2):239-61 - PubMed
  15. FASEB J. 1987 Nov;1(5):394-7 - PubMed
  16. Br J Cancer. 2006 Mar 27;94(6):884-90 - PubMed
  17. Crit Rev Food Sci Nutr. 2003;43(2):219-31 - PubMed
  18. Z Naturforsch C. 2007 Nov-Dec;62(11-12):889-96 - PubMed
  19. J Trace Elem Med Biol. 2000 Oct;14(3):168-73 - PubMed
  20. Biochem Biophys Res Commun. 2001 Apr 20;282(5):1103-9 - PubMed
  21. Mol Cell. 2004 Nov 5;16(3):331-41 - PubMed

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